Multicomponent meningococcal vaccine

ABSTRACT

A composition is provided comprising  N. meningitidis  outer membrane vesicles, wherein said outer membrane vesicles are enriched with at least one antigenic component. The composition is suitable for use in vaccines and for treatment of infection, particularly meningococcal infection, demonstrating a broad spectrum of protection. A number of preferred antigenic components are described and include antigenic proteins and proteoglycans derived from  N. meningitidis.

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This Application is a Continuation-in-Part of U.S. applicationSer. No. 09/830,854 with a 102(e) filing date of Aug. 29, 2001, which isa 371 of International Application No. PCT/GB99/03626 published underPCT Article 21(2) in English, with an International Filing date of Nov.2, 1999, which claims priority to British Patent Application No.9823978.3, filed Nov. 2, 1998, the contents of each of theseapplications are fully incorporated by reference.

BACKGROUND OF THE INVENTION Field of the Invention

[0002] The present invention relates to a multicomponent vaccine andmethods for preparing a multicomponent vaccine that confers protectiveimmunity to a broad spectrum of infection by Gram negative pathogens. Inparticular the present invention relates to a multicomponent vaccinethat provides both passive and active protective immunity tomeningococcal disease.

[0003] Meningococcal meningitis is a major problem worldwide and in manycountries incidence of infection is increasing. Neisseria meningitidisis the causative agent of the disease and is also responsible formeningococcal septicaemia, which is associated with rapid onset and highmortality, with around 22% of cases proving fatal.

[0004] At present, vaccines directed at providing protective immunityagainst meningococcal disease provide only limited protection because ofthe many different strains of N. meningitidis. Vaccines based upon theserogroup antigens, the capsular polysaccharides, offer only short livedprotection against infection and do not protect against many strainscommonly found in North America and Europe. A further drawback of thesevaccines is that they provide low levels of protection for childrenunder the age of 2 years, one of the most vulnerable groups that arecommonly susceptible to infection.

[0005] The meningococcal transferrin receptor is made up of two types ofcomponent protein chain, Transferrin binding protein A (TbpA) and TbpB.The receptor complex is believed to be formed from a dimer of TbpA whichassociates with a single TbpB (Boulton et al. (1998)). Epitopes presentin TbpA are known to be masked within the interior of the protein(Ala′Aldeen (1996)). Vaccines against meningococcal meningitis based onTbpB from one strain alone show some cross reactivity and there isevidence of a cross-reactive immune response in rabbits immunised withTbpB alone (Feirrerós et al. (1998)).

[0006] It would be of advantage, nevertheless, to provide a vaccine thatgives a broader range of protective immunity to infection from a widerspectrum of strains of N. meningitidis. It would be of further advantageto provide a vaccine that confers protective immunity to infants as wellas adults and whose protection is long term. It would also be ofadvantage to provide a vaccine that protects against sub-clinicalinfection, i.e. where symptoms of meningococcal infection are notimmediately apparent that the infected individual may act as a carrierof the pathogen.

[0007] It is an object of the present invention to provide compositionscontaining Tbps, and vaccines based thereon, that meet or at leastameliorate the disadvantages in the art. In particular, it is an objectof the invention to provide a vaccine composition that consistently andreliably induces protective immunity to meningococcal infection.

[0008] Accordingly, a first aspect of the present invention provides acomposition comprising both transferrin binding proteins A (TbpA) and B(TbpB), suitably in a molar ratio of about 2:1 (TbpA:TbpB). In apreferred embodiment of the present invention the molar ratio of TbpA toTbpB is 2:1.

[0009] The composition may be combined with a pharmaceuticallyacceptable carrier—for example the adjuvant alum although any carriersuitable for oral, intravenous, subcutaneous, intraperitoneal or anyother route of administration is suitable—to produce a pharmaceuticalcomposition for treatment of meningococcal disease.

[0010] The present invention thus provides for a vaccine comprising bothTbpA+B proteins, preferably with a molar ratio of between 1.8 and 2.2molecules of TbpA to one molecule of TbpB, more preferably 2 moleculesof TbpA to one of TbpB. This particular combination of components,surprisingly, can provide higher protective immunity to meningococcalinfection, compared to vaccination with TbpB alone. In a specificembodiment of the invention, described in more detail below, a 1:1combination of A:B is more protective against challenge than B alone.This is surprising as TbpA has previously been considered to benon-protective. The present results differ from this established viewwith some experiments (described in more detail below) showing that,when administered as a vaccine, TbpA is also able to provide protectiveimmunity to meningococcal infection. However, the present results moststrikingly demonstrate the consistent performance of vaccines thatcomprise both Tbps A and B compared to those comprising Tbp A or Balone. It is this lack of variability between compositions and theconsistently high level of protection to infection induced in responseto vaccination with Tbp A+B, that enables the compositions of theinvention to demonstrate significant advantage over the vaccines of theprior art.

[0011] Transferrin binding proteins are known to be located on the outermembranes of a number of Gram negative bacteria such as N. meningitidis.Formulations of the composition of the present invention withconventional carriers or adjuvants provide a composition for treatmentof infection by these bacteria.

[0012] It is an advantage that following administration of a compositionaccording to the present invention antibodies may be raised againstepitopes that consist of sequences from TbpA and TbpB in juxtaposition.Thus, the immune response obtainable using such a composition may beimproved compared with that from prior art vaccine compositions whichcomprise only one component of the Tbp complex and in which the fullrange of potential Tbp epitopes are unavailable. It is a further optionin the present invention for one Tbp subunit component of the TbpA+Bcomplex to be from a first strain of N. meningitidis and another from asecond strain different from the first. For example, the TbpA dimer istaken from the first strain and the TbpB is from the second. The TbpAand TbpB proteins may be selected independently from strains K454,H44/76 and B16B6. In all aspects of the invention the Tbps can bedirectly isolated from the bacterial source or can be produced byrecombinant methods commonly known in the art. Combinations of proteinsfrom other strains are also envisaged, and the combining of componentsfrom different strains of bacteria offers the potential for providing anindividual with a broader spectrum of protection against meningococcalinfection. It is further optional for a composition or vaccine of theinvention to contain a mixture of A proteins from different strains or amixture of B proteins from different strains, broadening further thepotential spectrum of protection conferred by the invention. A stillfurther option is for Tbps to be obtained from or derived from otherbacteria, including N.gonorrhoeae, N.lactamica and Moraxellacatarrhalis.

[0013] In the present invention, the term “transferrin binding protein”or “Tbp” refers to a protein which either alone binds to transferrin orcan be part of a complex of proteins that binds transferrin. The termalso embraces fragments, variants and derivatives of such a proteinprovided that antibodies raised against the fragment, variant orderivative bind the protein. Thus, TbpA and TbpB either dissociated orassociated into a complex are considered to be Tbp. Moreover, mutants,fusion proteins or fragments of either TbpA or B or other derivatives ofthe TbpA+B complex with a common antigenic identity are also consideredto be represented by the term Tbp in the present invention.

[0014] A second aspect of the invention provides a compositioncomprising a complex of two TbpAs and one TbpB. The proteins are thusheld together in the ratio seen in the native receptor. The individualproteins may be linked, for example, by hydrogen bonds or covalentbonds. In the latter case, each TbpA is covalently linked to the TbpA,either directly or indirectly. In a preferred embodiment, the complex ofTbpA and TbpB assumes a native configuration.

[0015] A native TbpA+B complex may be isolated and purified from N.meningitidis. Alternatively, the invention also provides for synthesisof recombinant Tbp protein followed by assembly of the TbpA+B complex invitro. The TbpA+B complex may be formed by admixture, or may becrosslinked by physical (e.g. UV radiation) or chemical methods known tothe art, resulting in a combination of Tbps that will remain togetherand can not dissociate from each other. In a further example, a singlechain recombinant protein comprising two TbpA sequences, preferably inthe form of the TbpA dimer, is then covalently linked with TbpB proteinto form a complete TbpA+B complex in vitro. Another example of theinvention in use provides that TbpA and B are mutated so as to introducecysteine residues that facilitate the formation of disulphide bridgesbetween the TbpA and TbpB subunits, so as to obtain a covalently boundcomplex.

[0016] In preparation of a recombinant protein TbpA and TbpB genes mayalso be truncated so that only those domains known to contribute to theantigenicity of the protein are incorporated in the Tbp complex.

[0017] In some compositions of the invention the TbpA+B complex is ableto act as a transferrin receptor and binds to human transferrin. Inother compositions of the invention the TbpA+B complex is non-functionalin the sense that it does not bind transferrin, but it neverthelessprovides an antigenic component that elicits an appropriate immuneresponse.

[0018] A third aspect of the invention provides for a compositioncomprising a Tbp and N. meningitidis outer membrane vesicles. Anadvantage of this composition is that when administered to a vaccinee orpatient it presents a different combination of N. meningitidis antigens,and particularly antigens that are in a formation substantially aspresent on the membrane of live infecting organisms. The combinationoffers the potential for a more effective protection against infectionor a broader spectrum of protection than existing vaccines. Knownmethods of outer membrane vesicle isolation, such as by desoxycholatetreatment, are suitable for preparation of compositions of theinvention. In various preferred embodiments the Tbp is Tbp A, Tbp B orTbpA+B either in a native complex or in dissociated form.

[0019] The outer membrane vesicles may further be pretreated in vitrowith Tbp so as to enrich the vesicle membrane with Tbp. By “enrich” andlike terms, we refer to an outer membrane vesicle to which has beenadded Tbps so as to increase the concentration or density of Tbps inthat vesicle. Preferably, the enrichment results in an outer membranevesicle having an increased number of transferrin receptors located inthe membrane, due to an increased concentration of TbpA and TbpBfollowing their addition to the vesicle and their association intoreceptors or receptor-like structures. A particular advantage of suchembodiments of the invention is that the Tbps, regarded as key antigeniccomponents of the vaccine, are presented in a highly antigenicenvironment that closely mimics the environment in which transferrinreceptors are presented on live, infecting bacteria.

[0020] As mentioned previously, the Tbp components of the compositionneed not be wild-type Tbps. They can be made recombinantly, and in sodoing sequence alterations may be introduced. In one typical example ofthe invention, recombinant TbpB is modified so as to comprise a membranebinding domain. A preferred membrane spanning domain is a hydrophobicalpha helical region added to either the N or C terminus of the Tbp Bprotein. However, a membrane anchoring region need not only be an alphahelix, addition of a fatty acid or lipid chain would also facilitatemembrane anchoring. In fact, wild type TbpB is believed to anchored tothe bacterial outer membrane via such a lipid chain anchor. In a furtherexample of the invention in use, the outer membrane vesicles arepretreated in vitro with membrane binding recombinant TbpB so as toenrich the vesicle membrane with TbpB. OMVs enriched with Tbps areadditionally generated by inducing high levels of Tbp expression in N.meningitidis and then isolating OMVs via one of the methods describedpreviously. This latter method is typically achieved by transforming theN. meningitidis host with a suitable expression vector into which hasbeen inserted a gene or genes encoding the Tbps of choice. Suitableexpression vectors for use in neisserial species include the plasmidpMGC10 (Nassif et al. (1991)).

[0021] It is preferred that the composition of the present inventioncomprises outer membrane vesicles and TbpA+B complexes isolated from arange of different strains of N. meningitidis. Other preferredcompositions of the invention comprise other N. meningitidis proteinsincluding surface antigens, periplasmic proteins, superoxide dismutaseand glycoproteins.

[0022] The composition of the third aspect of the invention may insteadof or in addition to outer membrane vesicles comprise one or moreliposomes, each liposome including TbpA and/or TbpB preferably includingTbpA and TbpB associated into a receptor or into a receptor-likecomplex. Thus a further means of presenting the transferrin receptorantigens is provided.

[0023] In a further embodiment of the third aspect of the invention, thecomposition comprises 22 kD antigen (Neisserial surface protein A(NspA)) as well as or instead of outer membrane vesicles. NspA and itspreparation are described by Martin et al, 1997.

[0024] A fourth aspect of the invention provides for a vaccinecomprising a composition of the invention as described above. A vaccineof the invention may also comprise antibodies to Tbp and thus provide alevel of passive immunity to bacterial infection.

[0025] A fifth aspect of the invention provides for a method ofmanufacturing a composition that comprises combining TbpA, TbpB and N.meningitidis outer membrane vesicles with a pharmaceutically acceptablecarrier. It is preferred that the molar ratio of TbpA to TbpB is about2:1. The outer membrane vesicles can be pretreated in vitro with nativeTbpA+B so as to enrich the vesicle membrane with Tbp complex. However,the outer membrane vesicles may also be pretreated with other proteincomponents so as to enrich them for these antigenic components also. Theouter membrane vesicles may also be pretreated with antigenic proteinsand proteoglycans from several different strains of N. meningitidis.

[0026] A further aspect of the invention provides for a compositioncomprising a Tbp and a Cu,Zn-Superoxide dismutase (Cu,Zn-SOD).

[0027] Cu,Zn-Superoxide Dismutase (Cu,Zn-SOD) is an metalloenzyme foundin many prokaryotic and eukaryotic organisms. It catalyses the reductionof the superoxide radical anion, O₂ ⁻, to hydrogen peroxide andmolecular oxygen, thus playing an important role in the removal ofcytotoxic free radicals from the organism. In bacteria Cu,Zn-SODs havebeen identified in the periplasm of a number of Gram negative speciesincluding N. meningitidis. The enzyme can exist as a dimer or a monomer,and accordingly in a preferred embodiment the present invention providesfor a composition comprising a Tbp and a Cu,Zn-SOD of the dimeric type.

[0028] As mentioned previously with regard to the use of the term “Tbp”,the Cu,Zn-SOD of the present invention is also considered to encompassfragments, variants and derivatives of such a protein provided thatantibodies raised against the fragment, variant or derivative bind thewild type Cu,Zn-SOD.

[0029] In examples of the invention in use, compositions are providedthat comprise a Cu,Zn-SOD and either a TbpA, a TbpB or a TbpA+B complex.In the latter example it is preferred that the molar ratio of TbpA toTbp B is between 1.8 and 2.2, with the most suitable compositions havinga ratio of 2:1 (TbpA:TbpB). In further compositions of the invention theTbps are from a different strain of N. meningitidis to that of theCu,Zn-SOD, thus facilitating the formation of a broader spectrum ofimmune response to meningococcal infection.

[0030] The invention also provides for compositions wherein the Tbps andCu,Zn-SOD are from different bacterial species, typically different Gramnegative species. Such compositions thereby allow an even broaderspectrum of immune response to be elicited when administered as avaccine. Typical compositions comprise a neisserial TbpA+B complex aswell as a monomeric Cu,Zn-SOD—from E. coli for example—and optionally afurther dimeric Cu,Zn-SOD—for example from Haemophilus parainfluenzae.

[0031] Other aspects of the invention provide for methods ofmanufacturing compositions that provide protective immunity to Gramnegative bacterial infection, comprising combining a covalently linkedcomplex of TbpA and TbpB with either or both of N. meningitidis outermembrane vesicles and a Cu,Zn-SOD, plus a pharmaceutically acceptablecarrier.

[0032] A further aspect provides for use of Tbps A and B in themanufacture of a medicament for human vaccination. It is preferred thatsuch a medicament is suitable for vaccination against meningococcalinfection, although some compositions of the invention provide broadspectrum protection to infection from a wider range of bacterialpathogens.

BRIEF DESCRIPTION OF THE FIGURES

[0033] The embodiments of the invention are discussed in more detail bymeans of the Example described below. The results referred to in theExample are illustrated by the accompanying drawings, in which:

[0034]FIG. 1 shows immunisation of mice with TbpA+B and outer membranevesicles;

[0035]FIG. 2 shows immunisation of mice with TbpA+B;

[0036]FIG. 3 shows protection of mice against IP infection afterimmunisation with TbpA+B, isolated TbpA or isolated TbpB;

[0037]FIGS. 4A and 4B show protection of mice against meningococcalinfection following immunisation with either neisserial TbpA+B, TbpB orTbpA (nTbps) or recombinant Tbps (rTbps);

[0038]FIGS. 5A and 5B show protection of mice against challenges of 10⁶and 10⁷ organisms/mouse of N. meningitidis strain K454 respectively,following immunisation with recombinant TbpA+B, TbpB or TbpA;

[0039]FIG. 6 shows protection against challenge with heterologousserogroup;

[0040]FIG. 7 shows protection against challenge with B16B6;

[0041]FIG. 8 shows insertion of a promoter construct into the N.meningitidis genome for up regulation of endogenous TbpB in N.meningitidis by homologous recombination; and

[0042]FIG. 9. shows insertion of an expression cassette into the N.meningitidis genome by homologous recombination.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0043] The invention is further illustrated by the followingnon-limiting examples.

EXAMPLES Example 1 Mouse Protective Data

[0044] Mice (CAMR-NIH) were immunised with Tbps and/or outer membranevesicles and challenged with either the homologous or a heterologousmeningococcal strain. Survivors per group of mice immunised with Tbpsfrom strain K454 and the outer membrane vesicle vaccine followingchallenge by strain K454 are shown in FIG. 1. Compared to Tbps, theouter membrane vesicle vaccine gave reduced protection but this may bebecause it is produced from a different group B strain (H44/76). Animalsimmunised with TbpA+B isolated from strain K454 were also protectedagainst challenge with other serogroup B organisms (FIG. 1), withgreater protection seen with the homologous strain and strainsexpressing a TbpB with a similar molecular weight. Little or noprotection was observed against challenge with meningococci possessingTbpB with a very different molecular weight (strain B16B6). With theheterologous challenge strains, there is a slightly greater numbersurvivors in the groups vaccinated with the combination of Tbps+outermembrane vesicles. However, the numbers involved are small and nodefinite conclusions can be reached. It is interesting to observe thatmice immunised with TbpA+B from strain K454 were also protected againstinfection with a serogroup C but not a serogroup A strain (FIG. 2).

[0045] Protection against challenge with strain K454 with mice immunisedwith co-purified TbpA+B and isolated TbpA and TbpB is shown in FIG. 3.It can be seen that TbpB is the predominant antigen responsible forprotection, with little protection afforded by TbpA alone.

[0046] Recombinant TbpA and TbpB

[0047] TbpA and TbpB from N. meningitidis strain K454 were cloned andoverexpressed in E. coli. The proteins were purified using affinitychromatography and used to determine their protective potency in a mousemodel of meningococcal disease. Recombinant Tbps showed equivalentprotection to that provided by Tbps isolated from iron-stressed N.meningitidis (FIGS. 4A and 4B). These recombinant Tbps were alsoutilised in two further larger IP challenge experiments (FIGS. 5A-B).

[0048] The strong and consistent protective potency of Tbps againstmouse I.P. infection with N. meningitidis is probably the mostcompelling evidence for their vaccine potential.

[0049] Interestingly, the results shown in FIGS. 4B and 5A-B, althougharising from experiments of similar design, show the surprisingvariability associated with vaccine compositions that depend solely uponeither TbpA or TbpB alone. In FIG. 4B the recombinant TbpA compositiondisplayed low levels of protection compared to TbpB or TbpA+B. However,in FIGS. 5A and 5B the recombinant TbpA vaccine composition that showedhigh levels of protection comparable to the TbpA+B complex and the TbpBalone composition demonstrated poor protection to infection. This latterresult is completely contrary to the teaching of the prior art.

[0050] Human Immune Response to TbpA and TbpB in Convalescent Sera

[0051] We have undertaken a number of studies looking at the antibodyresponse in humans to TbpA and TbpB following meningococcal disease. Thegeneral conclusions are that both TbpA and TbpB are expressed duringmeningococcal disease and that an immune response is raised againstthem. The response is functional (opsonic) and is more cross-reactivebetween different meningococcal strains than is the response induced byimmunisation of animals with Tbps. The immune response to TbpA appearsto be stronger and more cross-reactive than that to TbpB, confirming theimportance of the vaccine potential of TbpA.

[0052] TbpA and TbpB Form a Transferrin Receptor

[0053] Our structural studies indicate that the transferrin receptor onthe meningococcal surface consists of two TbpA molecules and one TbpBmolecule that act together.

[0054] Effect of a Vaccine Containing A+B Tbps

[0055] We carried out further tests of the efficacy of recombinant TbpBversus recombinant TbpA+B containing vaccine formulations againstchallenge from N. meningitidis strains L91 705 and B16B6, the results ofwhich are illustrated in FIGS. 6 and 7 respectively. In both cases someimproved protection was conferred by A+B compared to B alone.

Example 2 Up Regulation of Genes in Meningococcal Species

[0056] These methods can be used to enhance expression of TbpB but canequally be applied to other meningococcal outer membrane proteins withknown sequence such as NspA (Martin et al., 1997), OMP85 (Manning etal., 1998) or FrpB (Pettersson et al., 1995). Any of the antigeniccomponent sequences disclosed herein (SEQ ID NOS: 1; 3; 5; 7; 9; 11; 13;15; 17; 19; 21; 23; 25; 27; 29; 31; 33; 35; 37; 39; 41; 43; 45; 47; 49;51; 53; 55; 57; 59; 61; 63; 65; 67; 69; and 71) would also be suitablefor over expression in this system as would any immunogenicmeningococcal sequence.

[0057] In the following methods primers are designed using the primerselect program from the ‘DNA star’ software package (www.dnastar.com).Primers to flanking sequences are designed following sequencing from theknown coding region.

[0058] Up Regulation of TbpB in N. meningitidis by Promoter Delivery

[0059] Using homologous recombination (Frosch et al., 1990, van der Leyet al., 1995) a strong promoter such as the porA promoter is insertedinto the N. meningitidis genome upstream of the tbpB coding region(Legrain et al., 1993). A selectable marker, encoding resistance tokanamycin, is also included in the delivery cassette upstream from thepromoter. The integration locus is selected by sequencing upstream fromthe tbpB coding region.

[0060] PCR primers are designed to the regions flanking the porApromoter and incorporate restriction endonuclease sites for ligationinto this amplified sequence. This is then inserted into the multiplecloning site of a vector containing the kanamycin resistance gene. Alsoinserted through PCR produced products are integration loci thatcomplement a region directly before the coding region for tbpB in the N.meningitidis genome. Through homologous recombination the constructshown in FIG. 8 will be produced.

[0061] From the published sequence of porA (McGuinnes et al. 1990) (SEQID NO: 1) a primer is constructed at the N-terminal region in order tosequence up steam of the coding region. This sequence data yields thepromoter region of the highly expressed porA gene. Primers are designedto this sequence so as to amplify any conserved hexamers centred around−35 and −10 the consensus sequences of TTGACA and TATAAT with a 16-18 bpgap between them and up to 67 bp upstream of the start of transcription(ATG). The designed primers have restriction endonuclease sitesincorporated in order to insert the amplified region into a suitablevector.

[0062] In order to identify a suitable integration locus in front of thetbpB coding region a primer is designed to the start of the tbpB codingregion, using the published sequence (Legrain et al. 1993), running upstream into the tbpB promoter region. The promoter region itself as wellas some sequence upstream is suitable as the integration locus. Thissection is amplified in two halves using 4 primers and restrictionendonucleases are incorporated in order to ligate the sections into asuitable vector.

[0063] The vector used already contains an antibiotic resistance marker(such as kanamycin) and this is suitably positioned between theintegration loci but upstream from the incorporated porA promoter, asillustrated in FIG. 8. This means that the vector used has cloning siteseither side of the selectable marker.

[0064] Up Regulation of TbpB in N. meningitidis by Gene Delivery

[0065] A construct is produced containing a selectable marker, such askanamycin resistance, up stream from a strong promoter (porA) which isin turn upstream from the coding region for tbpB. Using homologousrecombination (Frosch et al., 1990, van der Ley et al., 1995), thecassette is inserted into the N. meningitidis genome. The integrationlocus is selected by sequencing upstream from a known sequenced areathat is also known to actively expressed.

[0066] PCR is used to amplify the tbpB coding region and porA promoterregion with suitable restriction endonucleases incorporated so they canbe inserted in series, into a vector containing kanamycin resistance.Also inserted into the construct are two flanking integration locicomplementing a region of the N. meningitidis genome (see FIG. 9).

[0067] From the published sequence of porA (McGuinness et al. 1990) (SEQID NO: 1) a primer can be constructed to complement the N-terminalregion in order to sequence up steam of the coding region. This sequencedata yields the promoter region of the highly expressed porA gene.Primers are designed to this sequence so as to amplify any conservedhexamers centred around −35 and −10 the consensus sequences of TTGACAand TATAAT with a 16-18 bp gap between them and up to 67 bp upstream ofthe start of transcription (ATG). The reverse primer is designed to beas close as possible to the start of the coding region, but does notactually include any of it. This ensures that when it is positioned inseries with any inserted DNA it acts as an efficient promoter. Thedesigned primers have restriction endonuclease sites incorporated inorder to insert the amplified region into a suitable vector.

[0068] From the published sequence of tbpB (Legrain et al. 1993) primersare constructed to complement the C-terminal and N-terminal regions inorder to sequence the flanking regions of the gene. From this sequenceprimers are constructed which amplify the entire gene. The forwardprimer incorporates the first coding triplet (ATG) of the gene and doesnot include the full native promoter region. The reverse is as close tothe end of the coding region as possible while still being a goodpartner to the forward primer. It is accepted that this means thereverse primer may be some distance downstream of the gene of interest.

[0069] From the Foregoing, it will be appreciated that although specificembodiments of the invention have been described herein for purposes ofillustration, various modifications may be made without deviating fromthe spirit and scope of the invention. Accordingly, the invention is notlimited except as by the appended claims.

1 75 1 1176 DNA Neisseria meningitidis CDS (1)..(1176) porA cds 1 atgcga aaa aaa ctt acc gcc ctc gta ttg tcc gca ctg ccg ctt gcg 48 Met ArgLys Lys Leu Thr Ala Leu Val Leu Ser Ala Leu Pro Leu Ala 1 5 10 15 gccgtt gcc gat gtc agc cta tac ggc gaa atc aaa gcc ggc gtg gaa 96 Ala ValAla Asp Val Ser Leu Tyr Gly Glu Ile Lys Ala Gly Val Glu 20 25 30 ggc aggaac tac cag ctg caa ttg act gaa gca caa gcc gct aac ggt 144 Gly Arg AsnTyr Gln Leu Gln Leu Thr Glu Ala Gln Ala Ala Asn Gly 35 40 45 gga gcg agcggt cag gta aaa gtt act aaa gtt act aag gcc aaa agc 192 Gly Ala Ser GlyGln Val Lys Val Thr Lys Val Thr Lys Ala Lys Ser 50 55 60 cgc atc agg acgaaa atc agt gat ttc ggc tcg ttt atc ggc ttt aag 240 Arg Ile Arg Thr LysIle Ser Asp Phe Gly Ser Phe Ile Gly Phe Lys 65 70 75 80 ggg agt gag gatttg ggc gac ggg ctg aag gct gtt tgg cag ctt gag 288 Gly Ser Glu Asp LeuGly Asp Gly Leu Lys Ala Val Trp Gln Leu Glu 85 90 95 caa gac gta tcc gttgcc ggc ggc ggc gcg acc cag tgg ggc aac agg 336 Gln Asp Val Ser Val AlaGly Gly Gly Ala Thr Gln Trp Gly Asn Arg 100 105 110 gaa tcc ttt atc ggcttg gca ggc gaa ttc ggt acg ctg cgc gcc ggt 384 Glu Ser Phe Ile Gly LeuAla Gly Glu Phe Gly Thr Leu Arg Ala Gly 115 120 125 cgc gtt gcg aat cagttt gac gat gcc agc caa gcc att gat cct tgg 432 Arg Val Ala Asn Gln PheAsp Asp Ala Ser Gln Ala Ile Asp Pro Trp 130 135 140 gac agc aat aat gatgtg gct tcg caa ttg ggt att ttc aaa cgc cac 480 Asp Ser Asn Asn Asp ValAla Ser Gln Leu Gly Ile Phe Lys Arg His 145 150 155 160 gac gac atg ccggtt tcc gta cgc tac gat tcc ccc gaa ttt tcc ggt 528 Asp Asp Met Pro ValSer Val Arg Tyr Asp Ser Pro Glu Phe Ser Gly 165 170 175 ttc agc ggc agcgtt caa ttc gtt ccg atc caa aac agc aag tcc gcc 576 Phe Ser Gly Ser ValGln Phe Val Pro Ile Gln Asn Ser Lys Ser Ala 180 185 190 tat acg ccg gcttat tat act aag aat aca aac aat aat ctt act ctc 624 Tyr Thr Pro Ala TyrTyr Thr Lys Asn Thr Asn Asn Asn Leu Thr Leu 195 200 205 gtt ccg gct gttgtc ggc aag ccc gga tcg gat gtg tat tat gcc ggt 672 Val Pro Ala Val ValGly Lys Pro Gly Ser Asp Val Tyr Tyr Ala Gly 210 215 220 ctg aat tac aaaaat ggc ggt ttt gcc ggg aac tat gcc ttt aaa tat 720 Leu Asn Tyr Lys AsnGly Gly Phe Ala Gly Asn Tyr Ala Phe Lys Tyr 225 230 235 240 gcg aga cacgcc aat gtc gga cgt aat gct ttt gag ttg ttc ttg atc 768 Ala Arg His AlaAsn Val Gly Arg Asn Ala Phe Glu Leu Phe Leu Ile 245 250 255 ggc agc gggagt gat caa gcc aaa ggt acc gat ccc ttg aaa aac cat 816 Gly Ser Gly SerAsp Gln Ala Lys Gly Thr Asp Pro Leu Lys Asn His 260 265 270 cag gta caccgt ctg acg ggc ggc tat gag gaa ggc ggc ttg aat ctc 864 Gln Val His ArgLeu Thr Gly Gly Tyr Glu Glu Gly Gly Leu Asn Leu 275 280 285 gcc ttg gcggct cag ttg gat ttg tct gaa aat ggc gac aaa acc aaa 912 Ala Leu Ala AlaGln Leu Asp Leu Ser Glu Asn Gly Asp Lys Thr Lys 290 295 300 aac agt acgacc gaa att gcc gcc act gct tcc tac cgc ttc ggt aat 960 Asn Ser Thr ThrGlu Ile Ala Ala Thr Ala Ser Tyr Arg Phe Gly Asn 305 310 315 320 gca gttcca cgc atc agc tat gcc cat ggt ttc gac ttt atc gaa cgc 1008 Ala Val ProArg Ile Ser Tyr Ala His Gly Phe Asp Phe Ile Glu Arg 325 330 335 ggt aaaaaa ggc gaa aat acc agc tac gat caa atc atc gcc ggc gtt 1056 Gly Lys LysGly Glu Asn Thr Ser Tyr Asp Gln Ile Ile Ala Gly Val 340 345 350 gat tatgat ttt tcc aaa cgc act tcc gcc atc gtg tct ggc gct tgg 1104 Asp Tyr AspPhe Ser Lys Arg Thr Ser Ala Ile Val Ser Gly Ala Trp 355 360 365 ctg aaacgc aat acc ggc atc ggc aac tac act caa att aat gcc gcc 1152 Leu Lys ArgAsn Thr Gly Ile Gly Asn Tyr Thr Gln Ile Asn Ala Ala 370 375 380 tcc gtcggt ttg cgc cac aaa ttc 1176 Ser Val Gly Leu Arg His Lys Phe 385 390 2392 PRT Neisseria meningitidis 2 Met Arg Lys Lys Leu Thr Ala Leu Val LeuSer Ala Leu Pro Leu Ala 1 5 10 15 Ala Val Ala Asp Val Ser Leu Tyr GlyGlu Ile Lys Ala Gly Val Glu 20 25 30 Gly Arg Asn Tyr Gln Leu Gln Leu ThrGlu Ala Gln Ala Ala Asn Gly 35 40 45 Gly Ala Ser Gly Gln Val Lys Val ThrLys Val Thr Lys Ala Lys Ser 50 55 60 Arg Ile Arg Thr Lys Ile Ser Asp PheGly Ser Phe Ile Gly Phe Lys 65 70 75 80 Gly Ser Glu Asp Leu Gly Asp GlyLeu Lys Ala Val Trp Gln Leu Glu 85 90 95 Gln Asp Val Ser Val Ala Gly GlyGly Ala Thr Gln Trp Gly Asn Arg 100 105 110 Glu Ser Phe Ile Gly Leu AlaGly Glu Phe Gly Thr Leu Arg Ala Gly 115 120 125 Arg Val Ala Asn Gln PheAsp Asp Ala Ser Gln Ala Ile Asp Pro Trp 130 135 140 Asp Ser Asn Asn AspVal Ala Ser Gln Leu Gly Ile Phe Lys Arg His 145 150 155 160 Asp Asp MetPro Val Ser Val Arg Tyr Asp Ser Pro Glu Phe Ser Gly 165 170 175 Phe SerGly Ser Val Gln Phe Val Pro Ile Gln Asn Ser Lys Ser Ala 180 185 190 TyrThr Pro Ala Tyr Tyr Thr Lys Asn Thr Asn Asn Asn Leu Thr Leu 195 200 205Val Pro Ala Val Val Gly Lys Pro Gly Ser Asp Val Tyr Tyr Ala Gly 210 215220 Leu Asn Tyr Lys Asn Gly Gly Phe Ala Gly Asn Tyr Ala Phe Lys Tyr 225230 235 240 Ala Arg His Ala Asn Val Gly Arg Asn Ala Phe Glu Leu Phe LeuIle 245 250 255 Gly Ser Gly Ser Asp Gln Ala Lys Gly Thr Asp Pro Leu LysAsn His 260 265 270 Gln Val His Arg Leu Thr Gly Gly Tyr Glu Glu Gly GlyLeu Asn Leu 275 280 285 Ala Leu Ala Ala Gln Leu Asp Leu Ser Glu Asn GlyAsp Lys Thr Lys 290 295 300 Asn Ser Thr Thr Glu Ile Ala Ala Thr Ala SerTyr Arg Phe Gly Asn 305 310 315 320 Ala Val Pro Arg Ile Ser Tyr Ala HisGly Phe Asp Phe Ile Glu Arg 325 330 335 Gly Lys Lys Gly Glu Asn Thr SerTyr Asp Gln Ile Ile Ala Gly Val 340 345 350 Asp Tyr Asp Phe Ser Lys ArgThr Ser Ala Ile Val Ser Gly Ala Trp 355 360 365 Leu Lys Arg Asn Thr GlyIle Gly Asn Tyr Thr Gln Ile Asn Ala Ala 370 375 380 Ser Val Gly Leu ArgHis Lys Phe 385 390 3 522 DNA Neisseria meningitidis CDS (1)..(522) NspAcds 3 atg aaa aaa gca ctt gcc aca ctg att gcc ctc gct ctc ccg gcc gcc 48Met Lys Lys Ala Leu Ala Thr Leu Ile Ala Leu Ala Leu Pro Ala Ala 1 5 1015 gca ctg gcg gaa ggc gca tcc ggc ttt tac gtc caa gcc gat gcc gca 96Ala Leu Ala Glu Gly Ala Ser Gly Phe Tyr Val Gln Ala Asp Ala Ala 20 25 30cac gca aaa gcc tca agc tct tta ggt tct gcc aaa ggc ttc agc ccg 144 HisAla Lys Ala Ser Ser Ser Leu Gly Ser Ala Lys Gly Phe Ser Pro 35 40 45 cgcatc tcc gca ggc tac cgc atc aac gac ctc cgc ttc gcc gtc gat 192 Arg IleSer Ala Gly Tyr Arg Ile Asn Asp Leu Arg Phe Ala Val Asp 50 55 60 tac acgcgc tac aaa aac tat aaa gcc cca tcc acc gat ttc aaa ctt 240 Tyr Thr ArgTyr Lys Asn Tyr Lys Ala Pro Ser Thr Asp Phe Lys Leu 65 70 75 80 tac agcatc ggc gcg tcc gcc att tac gac ttc gac acc caa tcg ccc 288 Tyr Ser IleGly Ala Ser Ala Ile Tyr Asp Phe Asp Thr Gln Ser Pro 85 90 95 gtc aaa ccgtat ctc ggc gcg cgc ttg agc ctc aac cgc gcc tcc gtc 336 Val Lys Pro TyrLeu Gly Ala Arg Leu Ser Leu Asn Arg Ala Ser Val 100 105 110 gac ttg ggcggc agc gac agc ttc agc caa acc tcc atc ggc ctc ggc 384 Asp Leu Gly GlySer Asp Ser Phe Ser Gln Thr Ser Ile Gly Leu Gly 115 120 125 gta ttg acgggc gta agc tat gcc gtt acc ccg aat gtc gat ttg gat 432 Val Leu Thr GlyVal Ser Tyr Ala Val Thr Pro Asn Val Asp Leu Asp 130 135 140 gcc ggc taccgc tac aac tac atc ggc aaa gtc aac act gtc aaa aac 480 Ala Gly Tyr ArgTyr Asn Tyr Ile Gly Lys Val Asn Thr Val Lys Asn 145 150 155 160 gtc cgttcc ggc gaa ctg tcc gcc ggt gtg cgc gtc aaa ttc 522 Val Arg Ser Gly GluLeu Ser Ala Gly Val Arg Val Lys Phe 165 170 4 174 PRT Neisseriameningitidis 4 Met Lys Lys Ala Leu Ala Thr Leu Ile Ala Leu Ala Leu ProAla Ala 1 5 10 15 Ala Leu Ala Glu Gly Ala Ser Gly Phe Tyr Val Gln AlaAsp Ala Ala 20 25 30 His Ala Lys Ala Ser Ser Ser Leu Gly Ser Ala Lys GlyPhe Ser Pro 35 40 45 Arg Ile Ser Ala Gly Tyr Arg Ile Asn Asp Leu Arg PheAla Val Asp 50 55 60 Tyr Thr Arg Tyr Lys Asn Tyr Lys Ala Pro Ser Thr AspPhe Lys Leu 65 70 75 80 Tyr Ser Ile Gly Ala Ser Ala Ile Tyr Asp Phe AspThr Gln Ser Pro 85 90 95 Val Lys Pro Tyr Leu Gly Ala Arg Leu Ser Leu AsnArg Ala Ser Val 100 105 110 Asp Leu Gly Gly Ser Asp Ser Phe Ser Gln ThrSer Ile Gly Leu Gly 115 120 125 Val Leu Thr Gly Val Ser Tyr Ala Val ThrPro Asn Val Asp Leu Asp 130 135 140 Ala Gly Tyr Arg Tyr Asn Tyr Ile GlyLys Val Asn Thr Val Lys Asn 145 150 155 160 Val Arg Ser Gly Glu Leu SerAla Gly Val Arg Val Lys Phe 165 170 5 2391 DNA Neisseria meningitidisCDS (1)..(2391) OMP85 cds 5 atg aaa ctg aaa cag att gct tcc gca ctg atgatg ttg ggc ata tcg 48 Met Lys Leu Lys Gln Ile Ala Ser Ala Leu Met MetLeu Gly Ile Ser 1 5 10 15 cct ttg gca ctt gcc gac ttc acc atc caa gacatc cgc gtc gaa ggc 96 Pro Leu Ala Leu Ala Asp Phe Thr Ile Gln Asp IleArg Val Glu Gly 20 25 30 ttg cag cgt acc gag ccg agt acc gta ttc aac tacctg ccc gtc aaa 144 Leu Gln Arg Thr Glu Pro Ser Thr Val Phe Asn Tyr LeuPro Val Lys 35 40 45 gtc ggc gac acc tac aac gac aca cac ggc agt gcc atcatc aaa agc 192 Val Gly Asp Thr Tyr Asn Asp Thr His Gly Ser Ala Ile IleLys Ser 50 55 60 ctg tac gcc acc ggt ttc ttt gac gac gta cgc gtc gaa actgcg gac 240 Leu Tyr Ala Thr Gly Phe Phe Asp Asp Val Arg Val Glu Thr AlaAsp 65 70 75 80 ggg cag ctc ctg ctg acc gtt atc gaa cgc ccc acc atc ggctcg ctc 288 Gly Gln Leu Leu Leu Thr Val Ile Glu Arg Pro Thr Ile Gly SerLeu 85 90 95 aac atc acc ggc gca aaa atg ctg caa aac gac gcc att aag aaaaac 336 Asn Ile Thr Gly Ala Lys Met Leu Gln Asn Asp Ala Ile Lys Lys Asn100 105 110 ctc gaa tcg ttc ggg ctg gcg cag tcg caa tac ttt aat cag gcgaca 384 Leu Glu Ser Phe Gly Leu Ala Gln Ser Gln Tyr Phe Asn Gln Ala Thr115 120 125 ctc aat cag gca gtc gcc ggc ctg aaa gaa gaa tac ctc ggg cgcggc 432 Leu Asn Gln Ala Val Ala Gly Leu Lys Glu Glu Tyr Leu Gly Arg Gly130 135 140 aaa ctc aat atc caa atc acg ccc aaa gta acc aaa ctc gcc cgcaac 480 Lys Leu Asn Ile Gln Ile Thr Pro Lys Val Thr Lys Leu Ala Arg Asn145 150 155 160 cgc gtc gac atc gac atc acg att gac gag ggc aaa tcc gccaaa atc 528 Arg Val Asp Ile Asp Ile Thr Ile Asp Glu Gly Lys Ser Ala LysIle 165 170 175 acc gac atc gaa ttt gaa ggc aac caa gtc tat tcc gac cgcaaa ctg 576 Thr Asp Ile Glu Phe Glu Gly Asn Gln Val Tyr Ser Asp Arg LysLeu 180 185 190 atg cgg caa atg tcc ctg acc gaa ggc ggc att tgg aca tggctg aca 624 Met Arg Gln Met Ser Leu Thr Glu Gly Gly Ile Trp Thr Trp LeuThr 195 200 205 cga agc aac caa ttc aac gag cag aaa ttt gcc caa gat atggaa aaa 672 Arg Ser Asn Gln Phe Asn Glu Gln Lys Phe Ala Gln Asp Met GluLys 210 215 220 gta acc gac ttc tac caa aat aac ggc tac ttc gat ttc cgtatc ctc 720 Val Thr Asp Phe Tyr Gln Asn Asn Gly Tyr Phe Asp Phe Arg IleLeu 225 230 235 240 gat acc gac atc caa acc aac gaa gac aaa acc aag cagacc atc aaa 768 Asp Thr Asp Ile Gln Thr Asn Glu Asp Lys Thr Lys Gln ThrIle Lys 245 250 255 atc acc gtc cac gaa ggc gga cgt ttc cgt tgg ggc aaagtc tcc atc 816 Ile Thr Val His Glu Gly Gly Arg Phe Arg Trp Gly Lys ValSer Ile 260 265 270 gaa ggc gac acc aac gaa gtc ccc aaa gcc gaa ctg gaaaaa ctg ctg 864 Glu Gly Asp Thr Asn Glu Val Pro Lys Ala Glu Leu Glu LysLeu Leu 275 280 285 acc atg aag ccc ggc aaa tgg tac gaa cgc cag cag atgacc gcc gtt 912 Thr Met Lys Pro Gly Lys Trp Tyr Glu Arg Gln Gln Met ThrAla Val 290 295 300 ttg ggt gag att cag aac cgc atg ggc tcg gca ggc tacgca tac agc 960 Leu Gly Glu Ile Gln Asn Arg Met Gly Ser Ala Gly Tyr AlaTyr Ser 305 310 315 320 gaa atc agc gta cag ccg ctg ccg aac gct gaa accaaa acc gtc gat 1008 Glu Ile Ser Val Gln Pro Leu Pro Asn Ala Glu Thr LysThr Val Asp 325 330 335 ttc gtc ctg cac atc gaa ccg ggc cgg aaa atc tacgtc aac gaa ata 1056 Phe Val Leu His Ile Glu Pro Gly Arg Lys Ile Tyr ValAsn Glu Ile 340 345 350 cac atc acc ggc aac aac aaa acc cgc gac gaa gtcgtc cgc cgt gaa 1104 His Ile Thr Gly Asn Asn Lys Thr Arg Asp Glu Val ValArg Arg Glu 355 360 365 tta cgc caa atg gaa tcc gca cct tac gac acc tccaag ctg caa cgt 1152 Leu Arg Gln Met Glu Ser Ala Pro Tyr Asp Thr Ser LysLeu Gln Arg 370 375 380 tcc aaa gag cgc gtc gag ctt ttg ggc tac ttc gacaat gtc cag ttt 1200 Ser Lys Glu Arg Val Glu Leu Leu Gly Tyr Phe Asp AsnVal Gln Phe 385 390 395 400 gat gct gtc ccg ctt gcc ggc acg ccc gac aaagtc gat ttg aac atg 1248 Asp Ala Val Pro Leu Ala Gly Thr Pro Asp Lys ValAsp Leu Asn Met 405 410 415 agt ctg acc gaa cgt tcc acc ggt tcc ctg gatttg agc gcg ggt tgg 1296 Ser Leu Thr Glu Arg Ser Thr Gly Ser Leu Asp LeuSer Ala Gly Trp 420 425 430 gtt caa gat acc ggg ttg gtc atg tcc gca ggcgtt tcc caa gac aac 1344 Val Gln Asp Thr Gly Leu Val Met Ser Ala Gly ValSer Gln Asp Asn 435 440 445 ctg ttc ggt acg ggc aag tcg gcc gca ctg cgcgcc tcc agg agc aaa 1392 Leu Phe Gly Thr Gly Lys Ser Ala Ala Leu Arg AlaSer Arg Ser Lys 450 455 460 acc acg ctt aac ggc tcg ctg tcg ttt act gacccg tac ttc acg gca 1440 Thr Thr Leu Asn Gly Ser Leu Ser Phe Thr Asp ProTyr Phe Thr Ala 465 470 475 480 gac ggg gtc agc ctg ggc tac gat gtt tacgga aaa gcc ttc gac ccg 1488 Asp Gly Val Ser Leu Gly Tyr Asp Val Tyr GlyLys Ala Phe Asp Pro 485 490 495 cgc aaa gca tcg acc agc atc aaa caa tataaa acc acc acg gca ggc 1536 Arg Lys Ala Ser Thr Ser Ile Lys Gln Tyr LysThr Thr Thr Ala Gly 500 505 510 gca ggc atc cgc atg agc gtg cct gtt accgaa tac gac cgc gtg aat 1584 Ala Gly Ile Arg Met Ser Val Pro Val Thr GluTyr Asp Arg Val Asn 515 520 525 ttc ggt ttg gtg gca gaa cac ctg acc gtcaac acc tac aac aaa gcg 1632 Phe Gly Leu Val Ala Glu His Leu Thr Val AsnThr Tyr Asn Lys Ala 530 535 540 ccc aaa cac tat gcc gac ttt atc aag aaatac ggc aaa acc gac ggc 1680 Pro Lys His Tyr Ala Asp Phe Ile Lys Lys TyrGly Lys Thr Asp Gly 545 550 555 560 aca gac ggc agc ttc aaa ggc tgg ctgtac aaa ggt acc gtc ggc tgg 1728 Thr Asp Gly Ser Phe Lys Gly Trp Leu TyrLys Gly Thr Val Gly Trp 565 570 575 ggg cgc aac aaa acc gac agc gcg ttatgg ccg acg cgc ggc tac ctg 1776 Gly Arg Asn Lys Thr Asp Ser Ala Leu TrpPro Thr Arg Gly Tyr Leu 580 585 590 acg ggc gtg aac gcc gaa atc gcc ctgcct ggc agc aaa ctg caa tac 1824 Thr Gly Val Asn Ala Glu Ile Ala Leu ProGly Ser Lys Leu Gln Tyr 595 600 605 tac tcc gcc acc cac aac caa acc tggttc ttc ccc ctg agc aaa acc 1872 Tyr Ser Ala Thr His Asn Gln Thr Trp PhePhe Pro Leu Ser Lys Thr 610 615 620 ttc acg ctg atg ctc ggc ggc gaa gtcggc att gcg ggc ggc tac ggc 1920 Phe Thr Leu Met Leu Gly Gly Glu Val GlyIle Ala Gly Gly Tyr Gly 625 630 635 640 aga acc aaa gaa atc ccc ttc tttgaa aac ttc tac ggc ggc ggc ctg 1968 Arg Thr Lys Glu Ile Pro Phe Phe GluAsn Phe Tyr Gly Gly Gly Leu 645 650 655 ggt tcg gtg cgc gga tac gaa agcggc acg ctc ggt ccg aaa gtc tat 2016 Gly Ser Val Arg Gly Tyr Glu Ser GlyThr Leu Gly Pro Lys Val Tyr 660 665 670 gac gaa tac ggc gaa aaa atc agctac ggc ggc aac aaa aaa gcc aac 2064 Asp Glu Tyr Gly Glu Lys Ile Ser TyrGly Gly Asn Lys Lys Ala Asn 675 680 685 gtc tcc gcc gag ctg ctc ttc ccgatg ccc ggc gcg aaa gac gcg cgc 2112 Val Ser Ala Glu Leu Leu Phe Pro MetPro Gly Ala Lys Asp Ala Arg 690 695 700 acc gtc cgc ctg agc ctg ttt gccgac gca ggc agc gtg tgg gac ggc 2160 Thr Val Arg Leu Ser Leu Phe Ala AspAla Gly Ser Val Trp Asp Gly 705 710 715 720 aaa acc tac gac gac aac agcagt tcc gcg acc ggc ggc agg gtt caa 2208 Lys Thr Tyr Asp Asp Asn Ser SerSer Ala Thr Gly Gly Arg Val Gln 725 730 735 aac att tac ggc gcc ggc aatacc cat aaa tcc acc ttt acc aac gaa 2256 Asn Ile Tyr Gly Ala Gly Asn ThrHis Lys Ser Thr Phe Thr Asn Glu 740 745 750 ttg cgc tat tcc gcc ggc ggcgcg gtt acc tgg ctc tcg cct tta ggc 2304 Leu Arg Tyr Ser Ala Gly Gly AlaVal Thr Trp Leu Ser Pro Leu Gly 755 760 765 ccg atg aaa ttc agc tac gcctac ccg ctg aag aaa aaa ccg gaa gac 2352 Pro Met Lys Phe Ser Tyr Ala TyrPro Leu Lys Lys Lys Pro Glu Asp 770 775 780 gaa atc caa cgc ttc caa ttccaa ctc ggc acg acg ttc 2391 Glu Ile Gln Arg Phe Gln Phe Gln Leu Gly ThrThr Phe 785 790 795 6 797 PRT Neisseria meningitidis 6 Met Lys Leu LysGln Ile Ala Ser Ala Leu Met Met Leu Gly Ile Ser 1 5 10 15 Pro Leu AlaLeu Ala Asp Phe Thr Ile Gln Asp Ile Arg Val Glu Gly 20 25 30 Leu Gln ArgThr Glu Pro Ser Thr Val Phe Asn Tyr Leu Pro Val Lys 35 40 45 Val Gly AspThr Tyr Asn Asp Thr His Gly Ser Ala Ile Ile Lys Ser 50 55 60 Leu Tyr AlaThr Gly Phe Phe Asp Asp Val Arg Val Glu Thr Ala Asp 65 70 75 80 Gly GlnLeu Leu Leu Thr Val Ile Glu Arg Pro Thr Ile Gly Ser Leu 85 90 95 Asn IleThr Gly Ala Lys Met Leu Gln Asn Asp Ala Ile Lys Lys Asn 100 105 110 LeuGlu Ser Phe Gly Leu Ala Gln Ser Gln Tyr Phe Asn Gln Ala Thr 115 120 125Leu Asn Gln Ala Val Ala Gly Leu Lys Glu Glu Tyr Leu Gly Arg Gly 130 135140 Lys Leu Asn Ile Gln Ile Thr Pro Lys Val Thr Lys Leu Ala Arg Asn 145150 155 160 Arg Val Asp Ile Asp Ile Thr Ile Asp Glu Gly Lys Ser Ala LysIle 165 170 175 Thr Asp Ile Glu Phe Glu Gly Asn Gln Val Tyr Ser Asp ArgLys Leu 180 185 190 Met Arg Gln Met Ser Leu Thr Glu Gly Gly Ile Trp ThrTrp Leu Thr 195 200 205 Arg Ser Asn Gln Phe Asn Glu Gln Lys Phe Ala GlnAsp Met Glu Lys 210 215 220 Val Thr Asp Phe Tyr Gln Asn Asn Gly Tyr PheAsp Phe Arg Ile Leu 225 230 235 240 Asp Thr Asp Ile Gln Thr Asn Glu AspLys Thr Lys Gln Thr Ile Lys 245 250 255 Ile Thr Val His Glu Gly Gly ArgPhe Arg Trp Gly Lys Val Ser Ile 260 265 270 Glu Gly Asp Thr Asn Glu ValPro Lys Ala Glu Leu Glu Lys Leu Leu 275 280 285 Thr Met Lys Pro Gly LysTrp Tyr Glu Arg Gln Gln Met Thr Ala Val 290 295 300 Leu Gly Glu Ile GlnAsn Arg Met Gly Ser Ala Gly Tyr Ala Tyr Ser 305 310 315 320 Glu Ile SerVal Gln Pro Leu Pro Asn Ala Glu Thr Lys Thr Val Asp 325 330 335 Phe ValLeu His Ile Glu Pro Gly Arg Lys Ile Tyr Val Asn Glu Ile 340 345 350 HisIle Thr Gly Asn Asn Lys Thr Arg Asp Glu Val Val Arg Arg Glu 355 360 365Leu Arg Gln Met Glu Ser Ala Pro Tyr Asp Thr Ser Lys Leu Gln Arg 370 375380 Ser Lys Glu Arg Val Glu Leu Leu Gly Tyr Phe Asp Asn Val Gln Phe 385390 395 400 Asp Ala Val Pro Leu Ala Gly Thr Pro Asp Lys Val Asp Leu AsnMet 405 410 415 Ser Leu Thr Glu Arg Ser Thr Gly Ser Leu Asp Leu Ser AlaGly Trp 420 425 430 Val Gln Asp Thr Gly Leu Val Met Ser Ala Gly Val SerGln Asp Asn 435 440 445 Leu Phe Gly Thr Gly Lys Ser Ala Ala Leu Arg AlaSer Arg Ser Lys 450 455 460 Thr Thr Leu Asn Gly Ser Leu Ser Phe Thr AspPro Tyr Phe Thr Ala 465 470 475 480 Asp Gly Val Ser Leu Gly Tyr Asp ValTyr Gly Lys Ala Phe Asp Pro 485 490 495 Arg Lys Ala Ser Thr Ser Ile LysGln Tyr Lys Thr Thr Thr Ala Gly 500 505 510 Ala Gly Ile Arg Met Ser ValPro Val Thr Glu Tyr Asp Arg Val Asn 515 520 525 Phe Gly Leu Val Ala GluHis Leu Thr Val Asn Thr Tyr Asn Lys Ala 530 535 540 Pro Lys His Tyr AlaAsp Phe Ile Lys Lys Tyr Gly Lys Thr Asp Gly 545 550 555 560 Thr Asp GlySer Phe Lys Gly Trp Leu Tyr Lys Gly Thr Val Gly Trp 565 570 575 Gly ArgAsn Lys Thr Asp Ser Ala Leu Trp Pro Thr Arg Gly Tyr Leu 580 585 590 ThrGly Val Asn Ala Glu Ile Ala Leu Pro Gly Ser Lys Leu Gln Tyr 595 600 605Tyr Ser Ala Thr His Asn Gln Thr Trp Phe Phe Pro Leu Ser Lys Thr 610 615620 Phe Thr Leu Met Leu Gly Gly Glu Val Gly Ile Ala Gly Gly Tyr Gly 625630 635 640 Arg Thr Lys Glu Ile Pro Phe Phe Glu Asn Phe Tyr Gly Gly GlyLeu 645 650 655 Gly Ser Val Arg Gly Tyr Glu Ser Gly Thr Leu Gly Pro LysVal Tyr 660 665 670 Asp Glu Tyr Gly Glu Lys Ile Ser Tyr Gly Gly Asn LysLys Ala Asn 675 680 685 Val Ser Ala Glu Leu Leu Phe Pro Met Pro Gly AlaLys Asp Ala Arg 690 695 700 Thr Val Arg Leu Ser Leu Phe Ala Asp Ala GlySer Val Trp Asp Gly 705 710 715 720 Lys Thr Tyr Asp Asp Asn Ser Ser SerAla Thr Gly Gly Arg Val Gln 725 730 735 Asn Ile Tyr Gly Ala Gly Asn ThrHis Lys Ser Thr Phe Thr Asn Glu 740 745 750 Leu Arg Tyr Ser Ala Gly GlyAla Val Thr Trp Leu Ser Pro Leu Gly 755 760 765 Pro Met Lys Phe Ser TyrAla Tyr Pro Leu Lys Lys Lys Pro Glu Asp 770 775 780 Glu Ile Gln Arg PheGln Phe Gln Leu Gly Thr Thr Phe 785 790 795 7 2142 DNA Neisseriameningitidis CDS (1)..(2142) FrpB cds 7 atg aat acc cca ttg ttc cgt ctcagc ctg ctc tcg ctt acc ctg gcg 48 Met Asn Thr Pro Leu Phe Arg Leu SerLeu Leu Ser Leu Thr Leu Ala 1 5 10 15 gca ggt ttt gcc cat gcg gca gaaaat aat gcc aag gtc gta ctg gat 96 Ala Gly Phe Ala His Ala Ala Glu AsnAsn Ala Lys Val Val Leu Asp 20 25 30 acc gtt acc gta aaa ggc gac cgc caaggc agc aaa atc cgt acc aac 144 Thr Val Thr Val Lys Gly Asp Arg Gln GlySer Lys Ile Arg Thr Asn 35 40 45 atc gtt acg ctg caa caa aaa gac gaa agcacc gca acc gat atg cgc 192 Ile Val Thr Leu Gln Gln Lys Asp Glu Ser ThrAla Thr Asp Met Arg 50 55 60 gaa ctc tta aaa gaa gag ccc tcc atc gat ttcggc ggc ggc aac ggc 240 Glu Leu Leu Lys Glu Glu Pro Ser Ile Asp Phe GlyGly Gly Asn Gly 65 70 75 80 acg tcc caa ttc ctg acg ctg cgc ggc atg ggtcaa aac tct gtc gac 288 Thr Ser Gln Phe Leu Thr Leu Arg Gly Met Gly GlnAsn Ser Val Asp 85 90 95 atc aag gtg gac aac gcc tat tcc gac agc caa atcctt tac cac caa 336 Ile Lys Val Asp Asn Ala Tyr Ser Asp Ser Gln Ile LeuTyr His Gln 100 105 110 ggc aga ttt att gtc gat ccc gct ttg gtt aaa gtcgtt tcc gta caa 384 Gly Arg Phe Ile Val Asp Pro Ala Leu Val Lys Val ValSer Val Gln 115 120 125 aaa ggc gcg ggt tcc gcc tct gcc ggt atc ggc gcgacc aac ggc gcg 432 Lys Gly Ala Gly Ser Ala Ser Ala Gly Ile Gly Ala ThrAsn Gly Ala 130 135 140 atc atc acc aaa acc gtc gat gcc caa gac ctg ctcaaa ggc ttg gat 480 Ile Ile Thr Lys Thr Val Asp Ala Gln Asp Leu Leu LysGly Leu Asp 145 150 155 160 aaa aac tgg ggc gtg cgc ctc aac agc ggc tttgcc agc aac gaa ggc 528 Lys Asn Trp Gly Val Arg Leu Asn Ser Gly Phe AlaSer Asn Glu Gly 165 170 175 gta agc tac ggc gca agc gta ttc ggg aaa gagggc aac ttc gac ggc 576 Val Ser Tyr Gly Ala Ser Val Phe Gly Lys Glu GlyAsn Phe Asp Gly 180 185 190 ttg ttc tct tac aac cgc aac aat gaa aaa gattac gaa gca ggt aaa 624 Leu Phe Ser Tyr Asn Arg Asn Asn Glu Lys Asp TyrGlu Ala Gly Lys 195 200 205 ggc ttc cgt aat aat ttc aac ggc ggc aaa accgta ccg tac agc gcg 672 Gly Phe Arg Asn Asn Phe Asn Gly Gly Lys Thr ValPro Tyr Ser Ala 210 215 220 ctg gac aaa cgc agc tac ctc gcc aaa atc ggaaca agc ttc ggc gac 720 Leu Asp Lys Arg Ser Tyr Leu Ala Lys Ile Gly ThrSer Phe Gly Asp 225 230 235 240 ggc gac cac cgc atc gta ttg agc cat atgaaa gac cag cac cgg ggc 768 Gly Asp His Arg Ile Val Leu Ser His Met LysAsp Gln His Arg Gly 245 250 255 atc cgt acc gtc cgt gaa gaa ttt acc gtcggc ggc gat aaa gag cga 816 Ile Arg Thr Val Arg Glu Glu Phe Thr Val GlyGly Asp Lys Glu Arg 260 265 270 ata agt atg gaa cgc caa gcc cct gct taccgc gaa acc aca caa tcc 864 Ile Ser Met Glu Arg Gln Ala Pro Ala Tyr ArgGlu Thr Thr Gln Ser 275 280 285 aac acc aat ttg gcg tac acg ggt aaa aacctg ggc ttt gtc gaa aaa 912 Asn Thr Asn Leu Ala Tyr Thr Gly Lys Asn LeuGly Phe Val Glu Lys 290 295 300 ctg gat gcc aac gcc tat gtg ttg gaa aaagaa cgc tat tcc gcc gat 960 Leu Asp Ala Asn Ala Tyr Val Leu Glu Lys GluArg Tyr Ser Ala Asp 305 310 315 320 gac agc ggc acc ggt tac gca ggc aatgta aaa ggc ccc aac cat acc 1008 Asp Ser Gly Thr Gly Tyr Ala Gly Asn ValLys Gly Pro Asn His Thr 325 330 335 caa atc acc act cgg ggt atg aac ttcaac ttc gac agc cgc ctt gcc 1056 Gln Ile Thr Thr Arg Gly Met Asn Phe AsnPhe Asp Ser Arg Leu Ala 340 345 350 gaa caa acc ctg ctg aaa tac ggt atcaac tac cgc cat cag gaa atc 1104 Glu Gln Thr Leu Leu Lys Tyr Gly Ile AsnTyr Arg His Gln Glu Ile 355 360 365 aaa ccg caa gcg ttt ttg aat tca caattt aaa att gaa gat aaa gaa 1152 Lys Pro Gln Ala Phe Leu Asn Ser Gln PheLys Ile Glu Asp Lys Glu 370 375 380 aaa gca act gat gaa gag aaa aat aagaac cgt gaa aat gaa aaa att 1200 Lys Ala Thr Asp Glu Glu Lys Asn Lys AsnArg Glu Asn Glu Lys Ile 385 390 395 400 gcc aaa gcc tac cgt ctg acc aacccg acc aaa acc gat acc ggc gcg 1248 Ala Lys Ala Tyr Arg Leu Thr Asn ProThr Lys Thr Asp Thr Gly Ala 405 410 415 tat atc gaa gcc att cac gag attgac ggc ttt acc ctg acc ggc ggg 1296 Tyr Ile Glu Ala Ile His Glu Ile AspGly Phe Thr Leu Thr Gly Gly 420 425 430 ctg cgt tac gac cgc ttc aag gtgaaa acc cac gac ggc aaa acc gtt 1344 Leu Arg Tyr Asp Arg Phe Lys Val LysThr His Asp Gly Lys Thr Val 435 440 445 tca agc aac aac ctt aac ccg agtttc ggc gtg att tgg cag ccg cac 1392 Ser Ser Asn Asn Leu Asn Pro Ser PheGly Val Ile Trp Gln Pro His 450 455 460 gaa cac tgg agc ttc agc gcg agccac aac tac gcc agc cgc agc ccg 1440 Glu His Trp Ser Phe Ser Ala Ser HisAsn Tyr Ala Ser Arg Ser Pro 465 470 475 480 cgc ctg tat gac gcg ctg caaacc cac ggc aaa cgc ggc atc atc tcg 1488 Arg Leu Tyr Asp Ala Leu Gln ThrHis Gly Lys Arg Gly Ile Ile Ser 485 490 495 att gcc gac ggc acg aaa gccgaa cgc gcg cgc aat acc gaa atc ggc 1536 Ile Ala Asp Gly Thr Lys Ala GluArg Ala Arg Asn Thr Glu Ile Gly 500 505 510 ttc aac tac aac gac ggc acgttt gcc gca aac ggc agc tac ttc tgg 1584 Phe Asn Tyr Asn Asp Gly Thr PheAla Ala Asn Gly Ser Tyr Phe Trp 515 520 525 cag acc atc aaa gac gcg cttgcc aat ccg caa aac cgc cac gac tct 1632 Gln Thr Ile Lys Asp Ala Leu AlaAsn Pro Gln Asn Arg His Asp Ser 530 535 540 gtc gcc gtc cgt gaa gcc gtcaat gcc ggt tac atc aaa aac cac ggt 1680 Val Ala Val Arg Glu Ala Val AsnAla Gly Tyr Ile Lys Asn His Gly 545 550 555 560 tac gaa ttg ggc gcg tcctac cgc acc ggc ggc ctg act gcc aaa gtc 1728 Tyr Glu Leu Gly Ala Ser TyrArg Thr Gly Gly Leu Thr Ala Lys Val 565 570 575 ggc gta agc cac agc aaaccg cgc ttt tac gat acg cac aaa gac aag 1776 Gly Val Ser His Ser Lys ProArg Phe Tyr Asp Thr His Lys Asp Lys 580 585 590 ctg ttg agc gcg aat cctgaa ttt ggc gca caa gtc ggc cgc act tgg 1824 Leu Leu Ser Ala Asn Pro GluPhe Gly Ala Gln Val Gly Arg Thr Trp 595 600 605 acg gct tcc ctt gcc taccgc ttc caa aac ccg aat ctg gaa atc ggc 1872 Thr Ala Ser Leu Ala Tyr ArgPhe Gln Asn Pro Asn Leu Glu Ile Gly 610 615 620 tgg cgc ggc cgt tat gttcaa aaa gcc gtg ggt tcg ata ttg gtg gca 1920 Trp Arg Gly Arg Tyr Val GlnLys Ala Val Gly Ser Ile Leu Val Ala 625 630 635 640 ggt caa aaa gac cgcaac ggc aaa ttg gaa aac gtt gta cgc aaa ggt 1968 Gly Gln Lys Asp Arg AsnGly Lys Leu Glu Asn Val Val Arg Lys Gly 645 650 655 ttc ggt gtg aac gatgtc ttc gcc aac tgg aaa ccg ctg ggc aaa gac 2016 Phe Gly Val Asn Asp ValPhe Ala Asn Trp Lys Pro Leu Gly Lys Asp 660 665 670 acg ctc aat gtt aatctt tcg gtt aac aac gtg ttc aac acg ttc tac 2064 Thr Leu Asn Val Asn LeuSer Val Asn Asn Val Phe Asn Thr Phe Tyr 675 680 685 tat ccg cac agc caacga tgg acc aat acc ctg ccg ggc gtg gga cgt 2112 Tyr Pro His Ser Gln ArgTrp Thr Asn Thr Leu Pro Gly Val Gly Arg 690 695 700 gat gta cgc ttg ggcgtg aac tac aag ttc 2142 Asp Val Arg Leu Gly Val Asn Tyr Lys Phe 705 7108 714 PRT Neisseria meningitidis 8 Met Asn Thr Pro Leu Phe Arg Leu SerLeu Leu Ser Leu Thr Leu Ala 1 5 10 15 Ala Gly Phe Ala His Ala Ala GluAsn Asn Ala Lys Val Val Leu Asp 20 25 30 Thr Val Thr Val Lys Gly Asp ArgGln Gly Ser Lys Ile Arg Thr Asn 35 40 45 Ile Val Thr Leu Gln Gln Lys AspGlu Ser Thr Ala Thr Asp Met Arg 50 55 60 Glu Leu Leu Lys Glu Glu Pro SerIle Asp Phe Gly Gly Gly Asn Gly 65 70 75 80 Thr Ser Gln Phe Leu Thr LeuArg Gly Met Gly Gln Asn Ser Val Asp 85 90 95 Ile Lys Val Asp Asn Ala TyrSer Asp Ser Gln Ile Leu Tyr His Gln 100 105 110 Gly Arg Phe Ile Val AspPro Ala Leu Val Lys Val Val Ser Val Gln 115 120 125 Lys Gly Ala Gly SerAla Ser Ala Gly Ile Gly Ala Thr Asn Gly Ala 130 135 140 Ile Ile Thr LysThr Val Asp Ala Gln Asp Leu Leu Lys Gly Leu Asp 145 150 155 160 Lys AsnTrp Gly Val Arg Leu Asn Ser Gly Phe Ala Ser Asn Glu Gly 165 170 175 ValSer Tyr Gly Ala Ser Val Phe Gly Lys Glu Gly Asn Phe Asp Gly 180 185 190Leu Phe Ser Tyr Asn Arg Asn Asn Glu Lys Asp Tyr Glu Ala Gly Lys 195 200205 Gly Phe Arg Asn Asn Phe Asn Gly Gly Lys Thr Val Pro Tyr Ser Ala 210215 220 Leu Asp Lys Arg Ser Tyr Leu Ala Lys Ile Gly Thr Ser Phe Gly Asp225 230 235 240 Gly Asp His Arg Ile Val Leu Ser His Met Lys Asp Gln HisArg Gly 245 250 255 Ile Arg Thr Val Arg Glu Glu Phe Thr Val Gly Gly AspLys Glu Arg 260 265 270 Ile Ser Met Glu Arg Gln Ala Pro Ala Tyr Arg GluThr Thr Gln Ser 275 280 285 Asn Thr Asn Leu Ala Tyr Thr Gly Lys Asn LeuGly Phe Val Glu Lys 290 295 300 Leu Asp Ala Asn Ala Tyr Val Leu Glu LysGlu Arg Tyr Ser Ala Asp 305 310 315 320 Asp Ser Gly Thr Gly Tyr Ala GlyAsn Val Lys Gly Pro Asn His Thr 325 330 335 Gln Ile Thr Thr Arg Gly MetAsn Phe Asn Phe Asp Ser Arg Leu Ala 340 345 350 Glu Gln Thr Leu Leu LysTyr Gly Ile Asn Tyr Arg His Gln Glu Ile 355 360 365 Lys Pro Gln Ala PheLeu Asn Ser Gln Phe Lys Ile Glu Asp Lys Glu 370 375 380 Lys Ala Thr AspGlu Glu Lys Asn Lys Asn Arg Glu Asn Glu Lys Ile 385 390 395 400 Ala LysAla Tyr Arg Leu Thr Asn Pro Thr Lys Thr Asp Thr Gly Ala 405 410 415 TyrIle Glu Ala Ile His Glu Ile Asp Gly Phe Thr Leu Thr Gly Gly 420 425 430Leu Arg Tyr Asp Arg Phe Lys Val Lys Thr His Asp Gly Lys Thr Val 435 440445 Ser Ser Asn Asn Leu Asn Pro Ser Phe Gly Val Ile Trp Gln Pro His 450455 460 Glu His Trp Ser Phe Ser Ala Ser His Asn Tyr Ala Ser Arg Ser Pro465 470 475 480 Arg Leu Tyr Asp Ala Leu Gln Thr His Gly Lys Arg Gly IleIle Ser 485 490 495 Ile Ala Asp Gly Thr Lys Ala Glu Arg Ala Arg Asn ThrGlu Ile Gly 500 505 510 Phe Asn Tyr Asn Asp Gly Thr Phe Ala Ala Asn GlySer Tyr Phe Trp 515 520 525 Gln Thr Ile Lys Asp Ala Leu Ala Asn Pro GlnAsn Arg His Asp Ser 530 535 540 Val Ala Val Arg Glu Ala Val Asn Ala GlyTyr Ile Lys Asn His Gly 545 550 555 560 Tyr Glu Leu Gly Ala Ser Tyr ArgThr Gly Gly Leu Thr Ala Lys Val 565 570 575 Gly Val Ser His Ser Lys ProArg Phe Tyr Asp Thr His Lys Asp Lys 580 585 590 Leu Leu Ser Ala Asn ProGlu Phe Gly Ala Gln Val Gly Arg Thr Trp 595 600 605 Thr Ala Ser Leu AlaTyr Arg Phe Gln Asn Pro Asn Leu Glu Ile Gly 610 615 620 Trp Arg Gly ArgTyr Val Gln Lys Ala Val Gly Ser Ile Leu Val Ala 625 630 635 640 Gly GlnLys Asp Arg Asn Gly Lys Leu Glu Asn Val Val Arg Lys Gly 645 650 655 PheGly Val Asn Asp Val Phe Ala Asn Trp Lys Pro Leu Gly Lys Asp 660 665 670Thr Leu Asn Val Asn Leu Ser Val Asn Asn Val Phe Asn Thr Phe Tyr 675 680685 Tyr Pro His Ser Gln Arg Trp Thr Asn Thr Leu Pro Gly Val Gly Arg 690695 700 Asp Val Arg Leu Gly Val Asn Tyr Lys Phe 705 710 9 2307 DNANeisseria meningitidis CDS (1)..(2307) PilQ cds 9 atg aat acc aaa ctgaca aaa atc att tcc ggt ctc ttt gtc gca acc 48 Met Asn Thr Lys Leu ThrLys Ile Ile Ser Gly Leu Phe Val Ala Thr 1 5 10 15 gcc gcc ttt cag acagca tcg gca gga aac att aca gac atc aaa gtt 96 Ala Ala Phe Gln Thr AlaSer Ala Gly Asn Ile Thr Asp Ile Lys Val 20 25 30 tcc tcc ctg ccc aac aaacag aaa atc gtc aaa gtc agc ttt gac aaa 144 Ser Ser Leu Pro Asn Lys GlnLys Ile Val Lys Val Ser Phe Asp Lys 35 40 45 gag att gtc aac ccg acc ggcttc gta acc tcc tca ccg gcc cgc atc 192 Glu Ile Val Asn Pro Thr Gly PheVal Thr Ser Ser Pro Ala Arg Ile 50 55 60 gcc ttg gac ttt gaa caa acc ggcatt tcc atg gat caa cag gta ctc 240 Ala Leu Asp Phe Glu Gln Thr Gly IleSer Met Asp Gln Gln Val Leu 65 70 75 80 gaa tat gcc gat cct ctg ttg agcaaa atc agt gcc gca caa aac agc 288 Glu Tyr Ala Asp Pro Leu Leu Ser LysIle Ser Ala Ala Gln Asn Ser 85 90 95 agc cgt gcg cgt ctg gtt ctg aat ctgaac aaa ccg ggc caa tac aat 336 Ser Arg Ala Arg Leu Val Leu Asn Leu AsnLys Pro Gly Gln Tyr Asn 100 105 110 acc gaa gta cgc ggg aac aaa gtt tggata ttc att aac gaa tcg gac 384 Thr Glu Val Arg Gly Asn Lys Val Trp IlePhe Ile Asn Glu Ser Asp 115 120 125 gat acc gtg tcc gcc ccc gca cgc cccgcc gta aaa gcc gcg cct gcc 432 Asp Thr Val Ser Ala Pro Ala Arg Pro AlaVal Lys Ala Ala Pro Ala 130 135 140 gca ccg gca aaa caa cag gct gcc gcaccg tct acc aag tcc gca gta 480 Ala Pro Ala Lys Gln Gln Ala Ala Ala ProSer Thr Lys Ser Ala Val 145 150 155 160 tcc gta tcc gaa ccc ttt acc ccggca aaa caa cag gct gcc gca ccg 528 Ser Val Ser Glu Pro Phe Thr Pro AlaLys Gln Gln Ala Ala Ala Pro 165 170 175 ttt acc gag tcc gta gta tcc gtatcc gca ccg ttc agc ccg gca aaa 576 Phe Thr Glu Ser Val Val Ser Val SerAla Pro Phe Ser Pro Ala Lys 180 185 190 caa cag gcg gcg gca tca gca aaacaa cag gcg gca gca cca gca aaa 624 Gln Gln Ala Ala Ala Ser Ala Lys GlnGln Ala Ala Ala Pro Ala Lys 195 200 205 caa cag gcg gca gca cca gca aaacaa cag gcg gca gca cca gca aaa 672 Gln Gln Ala Ala Ala Pro Ala Lys GlnGln Ala Ala Ala Pro Ala Lys 210 215 220 caa acc aat atc gat ttc cgc aaagac ggc aaa aat gcc ggc att atc 720 Gln Thr Asn Ile Asp Phe Arg Lys AspGly Lys Asn Ala Gly Ile Ile 225 230 235 240 gaa ttg gct gca ttg ggc tttgcc ggg cag ccc gac atc agc caa cag 768 Glu Leu Ala Ala Leu Gly Phe AlaGly Gln Pro Asp Ile Ser Gln Gln 245 250 255 cac gac cac atc atc gtt acgctg aaa aac cat acc ctg ccg acc acg 816 His Asp His Ile Ile Val Thr LeuLys Asn His Thr Leu Pro Thr Thr 260 265 270 ctc caa cgc agt ttg gat gtggca gac ttt aaa aca ccg gtt caa aag 864 Leu Gln Arg Ser Leu Asp Val AlaAsp Phe Lys Thr Pro Val Gln Lys 275 280 285 gtt acg ctg aaa cgc ctc aataac gac acc cag ctg att atc aca aca 912 Val Thr Leu Lys Arg Leu Asn AsnAsp Thr Gln Leu Ile Ile Thr Thr 290 295 300 gcc ggc aac tgg gaa ctc gtcaac aaa tcc gcc gcg ccc gga tac ttt 960 Ala Gly Asn Trp Glu Leu Val AsnLys Ser Ala Ala Pro Gly Tyr Phe 305 310 315 320 acc ttc caa gtc ctg ccgaaa aaa caa aac ctc gag tca ggc ggc gtg 1008 Thr Phe Gln Val Leu Pro LysLys Gln Asn Leu Glu Ser Gly Gly Val 325 330 335 aac aat gcg ccc aaa accttc aca ggc cgg aaa atc tcc ctt gac ttc 1056 Asn Asn Ala Pro Lys Thr PheThr Gly Arg Lys Ile Ser Leu Asp Phe 340 345 350 caa gat gtc gaa atc cgcacc atc ctg cag att ttg gca aaa gaa tcc 1104 Gln Asp Val Glu Ile Arg ThrIle Leu Gln Ile Leu Ala Lys Glu Ser 355 360 365 gga atg aac att gtt gccagc gac tcc gtc aac ggc aaa atg acc ctc 1152 Gly Met Asn Ile Val Ala SerAsp Ser Val Asn Gly Lys Met Thr Leu 370 375 380 tcc ctc aag gat gtg ccttgg gat cag gct ttg gat ttg gtt atg cag 1200 Ser Leu Lys Asp Val Pro TrpAsp Gln Ala Leu Asp Leu Val Met Gln 385 390 395 400 gcg cgc aac ctc gatatg cgc cag caa ggg aat atc gtc aac atc gcg 1248 Ala Arg Asn Leu Asp MetArg Gln Gln Gly Asn Ile Val Asn Ile Ala 405 410 415 ccc cgc gac gag ctgctt gcc aaa gac aaa gcc ctc tta cag gca gaa 1296 Pro Arg Asp Glu Leu LeuAla Lys Asp Lys Ala Leu Leu Gln Ala Glu 420 425 430 aaa gac att gcc gatttg ggt gcg ctg tat tcc caa aac ttc cag ttg 1344 Lys Asp Ile Ala Asp LeuGly Ala Leu Tyr Ser Gln Asn Phe Gln Leu 435 440 445 aaa tac aaa aat gtggaa gaa ttc cgc agc atc ctg cgt ttg gac aat 1392 Lys Tyr Lys Asn Val GluGlu Phe Arg Ser Ile Leu Arg Leu Asp Asn 450 455 460 gcc gac acg acc ggaaac cgc aac acg ctt atc agc ggc agg ggc agc 1440 Ala Asp Thr Thr Gly AsnArg Asn Thr Leu Ile Ser Gly Arg Gly Ser 465 470 475 480 gtg ctg atc gatccc gcc acc aac acc ctg att gtt acc gac acc cgc 1488 Val Leu Ile Asp ProAla Thr Asn Thr Leu Ile Val Thr Asp Thr Arg 485 490 495 agc gtc atc gaaaaa ttc cgc aaa ctg att gac gaa ttg gac gta ccc 1536 Ser Val Ile Glu LysPhe Arg Lys Leu Ile Asp Glu Leu Asp Val Pro 500 505 510 gcg caa caa gtgatg att gag gcg cgt atc gtc gaa gcg gca gac ggc 1584 Ala Gln Gln Val MetIle Glu Ala Arg Ile Val Glu Ala Ala Asp Gly 515 520 525 ttc tcg cgc gatttg ggc gtt aaa ttc ggc gcg aca ggc aag aaa aag 1632 Phe Ser Arg Asp LeuGly Val Lys Phe Gly Ala Thr Gly Lys Lys Lys 530 535 540 ctg aaa aat gataca agc gca ttc ggc tgg ggg gta aac tcc ggc ttc 1680 Leu Lys Asn Asp ThrSer Ala Phe Gly Trp Gly Val Asn Ser Gly Phe 545 550 555 560 ggc ggc gacgat aaa tgg ggg gcc gaa acc aaa atc aac ctg ccg att 1728 Gly Gly Asp AspLys Trp Gly Ala Glu Thr Lys Ile Asn Leu Pro Ile 565 570 575 acc gct gccgca aac agc att tcg ctg gtg cgc gcg att tcc tcc ggt 1776 Thr Ala Ala AlaAsn Ser Ile Ser Leu Val Arg Ala Ile Ser Ser Gly 580 585 590 gcc ttg aatttg gaa ttg tcc gca tcc gaa tcg ctt tca aaa acc aaa 1824 Ala Leu Asn LeuGlu Leu Ser Ala Ser Glu Ser Leu Ser Lys Thr Lys 595 600 605 acg ctt gccaat ccg cgc gtg ctg acc caa aac cgc aaa gag gcc aaa 1872 Thr Leu Ala AsnPro Arg Val Leu Thr Gln Asn Arg Lys Glu Ala Lys 610 615 620 atc gaa tccggt tac gaa att cct ttc acc gta acc tca atc gcg aac 1920 Ile Glu Ser GlyTyr Glu Ile Pro Phe Thr Val Thr Ser Ile Ala Asn 625 630 635 640 ggc ggcagc agc acg aac acg gaa ctc aaa aaa gcc gtc ttg ggg ctg 1968 Gly Gly SerSer Thr Asn Thr Glu Leu Lys Lys Ala Val Leu Gly Leu 645 650 655 acc gttacg ccg aac atc acg ccc gac ggc caa atc att atg acc gtc 2016 Thr Val ThrPro Asn Ile Thr Pro Asp Gly Gln Ile Ile Met Thr Val 660 665 670 aaa atcaac aag gac tcg cct gcg caa tgt gcc tcc ggt aat cag acg 2064 Lys Ile AsnLys Asp Ser Pro Ala Gln Cys Ala Ser Gly Asn Gln Thr 675 680 685 atc ctgtgt att tcg acc aaa aac ctg aat acg cag gct atg gtt gaa 2112 Ile Leu CysIle Ser Thr Lys Asn Leu Asn Thr Gln Ala Met Val Glu 690 695 700 aac ggcggc aca ttg att gtc ggc ggt att tat gaa gaa gac aac ggc 2160 Asn Gly GlyThr Leu Ile Val Gly Gly Ile Tyr Glu Glu Asp Asn Gly 705 710 715 720 aatacg ctg acc aaa gtc ccc ctg ttg ggc gac atc ccc gtt atc ggc 2208 Asn ThrLeu Thr Lys Val Pro Leu Leu Gly Asp Ile Pro Val Ile Gly 725 730 735 aacctc ttt aaa aca cgc ggg aaa aaa acc gac cgc cgc gaa ctg ctg 2256 Asn LeuPhe Lys Thr Arg Gly Lys Lys Thr Asp Arg Arg Glu Leu Leu 740 745 750 attttc att acc ccg agg att atg ggt acg gcc ggc aac agc ctg cgc 2304 Ile PheIle Thr Pro Arg Ile Met Gly Thr Ala Gly Asn Ser Leu Arg 755 760 765 tat2307 Tyr 10 769 PRT Neisseria meningitidis 10 Met Asn Thr Lys Leu ThrLys Ile Ile Ser Gly Leu Phe Val Ala Thr 1 5 10 15 Ala Ala Phe Gln ThrAla Ser Ala Gly Asn Ile Thr Asp Ile Lys Val 20 25 30 Ser Ser Leu Pro AsnLys Gln Lys Ile Val Lys Val Ser Phe Asp Lys 35 40 45 Glu Ile Val Asn ProThr Gly Phe Val Thr Ser Ser Pro Ala Arg Ile 50 55 60 Ala Leu Asp Phe GluGln Thr Gly Ile Ser Met Asp Gln Gln Val Leu 65 70 75 80 Glu Tyr Ala AspPro Leu Leu Ser Lys Ile Ser Ala Ala Gln Asn Ser 85 90 95 Ser Arg Ala ArgLeu Val Leu Asn Leu Asn Lys Pro Gly Gln Tyr Asn 100 105 110 Thr Glu ValArg Gly Asn Lys Val Trp Ile Phe Ile Asn Glu Ser Asp 115 120 125 Asp ThrVal Ser Ala Pro Ala Arg Pro Ala Val Lys Ala Ala Pro Ala 130 135 140 AlaPro Ala Lys Gln Gln Ala Ala Ala Pro Ser Thr Lys Ser Ala Val 145 150 155160 Ser Val Ser Glu Pro Phe Thr Pro Ala Lys Gln Gln Ala Ala Ala Pro 165170 175 Phe Thr Glu Ser Val Val Ser Val Ser Ala Pro Phe Ser Pro Ala Lys180 185 190 Gln Gln Ala Ala Ala Ser Ala Lys Gln Gln Ala Ala Ala Pro AlaLys 195 200 205 Gln Gln Ala Ala Ala Pro Ala Lys Gln Gln Ala Ala Ala ProAla Lys 210 215 220 Gln Thr Asn Ile Asp Phe Arg Lys Asp Gly Lys Asn AlaGly Ile Ile 225 230 235 240 Glu Leu Ala Ala Leu Gly Phe Ala Gly Gln ProAsp Ile Ser Gln Gln 245 250 255 His Asp His Ile Ile Val Thr Leu Lys AsnHis Thr Leu Pro Thr Thr 260 265 270 Leu Gln Arg Ser Leu Asp Val Ala AspPhe Lys Thr Pro Val Gln Lys 275 280 285 Val Thr Leu Lys Arg Leu Asn AsnAsp Thr Gln Leu Ile Ile Thr Thr 290 295 300 Ala Gly Asn Trp Glu Leu ValAsn Lys Ser Ala Ala Pro Gly Tyr Phe 305 310 315 320 Thr Phe Gln Val LeuPro Lys Lys Gln Asn Leu Glu Ser Gly Gly Val 325 330 335 Asn Asn Ala ProLys Thr Phe Thr Gly Arg Lys Ile Ser Leu Asp Phe 340 345 350 Gln Asp ValGlu Ile Arg Thr Ile Leu Gln Ile Leu Ala Lys Glu Ser 355 360 365 Gly MetAsn Ile Val Ala Ser Asp Ser Val Asn Gly Lys Met Thr Leu 370 375 380 SerLeu Lys Asp Val Pro Trp Asp Gln Ala Leu Asp Leu Val Met Gln 385 390 395400 Ala Arg Asn Leu Asp Met Arg Gln Gln Gly Asn Ile Val Asn Ile Ala 405410 415 Pro Arg Asp Glu Leu Leu Ala Lys Asp Lys Ala Leu Leu Gln Ala Glu420 425 430 Lys Asp Ile Ala Asp Leu Gly Ala Leu Tyr Ser Gln Asn Phe GlnLeu 435 440 445 Lys Tyr Lys Asn Val Glu Glu Phe Arg Ser Ile Leu Arg LeuAsp Asn 450 455 460 Ala Asp Thr Thr Gly Asn Arg Asn Thr Leu Ile Ser GlyArg Gly Ser 465 470 475 480 Val Leu Ile Asp Pro Ala Thr Asn Thr Leu IleVal Thr Asp Thr Arg 485 490 495 Ser Val Ile Glu Lys Phe Arg Lys Leu IleAsp Glu Leu Asp Val Pro 500 505 510 Ala Gln Gln Val Met Ile Glu Ala ArgIle Val Glu Ala Ala Asp Gly 515 520 525 Phe Ser Arg Asp Leu Gly Val LysPhe Gly Ala Thr Gly Lys Lys Lys 530 535 540 Leu Lys Asn Asp Thr Ser AlaPhe Gly Trp Gly Val Asn Ser Gly Phe 545 550 555 560 Gly Gly Asp Asp LysTrp Gly Ala Glu Thr Lys Ile Asn Leu Pro Ile 565 570 575 Thr Ala Ala AlaAsn Ser Ile Ser Leu Val Arg Ala Ile Ser Ser Gly 580 585 590 Ala Leu AsnLeu Glu Leu Ser Ala Ser Glu Ser Leu Ser Lys Thr Lys 595 600 605 Thr LeuAla Asn Pro Arg Val Leu Thr Gln Asn Arg Lys Glu Ala Lys 610 615 620 IleGlu Ser Gly Tyr Glu Ile Pro Phe Thr Val Thr Ser Ile Ala Asn 625 630 635640 Gly Gly Ser Ser Thr Asn Thr Glu Leu Lys Lys Ala Val Leu Gly Leu 645650 655 Thr Val Thr Pro Asn Ile Thr Pro Asp Gly Gln Ile Ile Met Thr Val660 665 670 Lys Ile Asn Lys Asp Ser Pro Ala Gln Cys Ala Ser Gly Asn GlnThr 675 680 685 Ile Leu Cys Ile Ser Thr Lys Asn Leu Asn Thr Gln Ala MetVal Glu 690 695 700 Asn Gly Gly Thr Leu Ile Val Gly Gly Ile Tyr Glu GluAsp Asn Gly 705 710 715 720 Asn Thr Leu Thr Lys Val Pro Leu Leu Gly AspIle Pro Val Ile Gly 725 730 735 Asn Leu Phe Lys Thr Arg Gly Lys Lys ThrAsp Arg Arg Glu Leu Leu 740 745 750 Ile Phe Ile Thr Pro Arg Ile Met GlyThr Ala Gly Asn Ser Leu Arg 755 760 765 Tyr 11 1773 DNA Neisseriameningitidis CDS (1)..(1773) Hsf cds 11 atg aac aaa ata tac cgc atc atttgg aat agt gcc ctc aat gcc tgg 48 Met Asn Lys Ile Tyr Arg Ile Ile TrpAsn Ser Ala Leu Asn Ala Trp 1 5 10 15 gtc gtc gta tcc gag ctc aca cgcaac cac acc aaa cgc gcc tcc gca 96 Val Val Val Ser Glu Leu Thr Arg AsnHis Thr Lys Arg Ala Ser Ala 20 25 30 acc gtg aag acc gcc gta ttg gcg acactg ttg ttt gca acg gtt cag 144 Thr Val Lys Thr Ala Val Leu Ala Thr LeuLeu Phe Ala Thr Val Gln 35 40 45 gca agt gct aac aat gaa gag caa gaa gaagat tta tat tta gac ccc 192 Ala Ser Ala Asn Asn Glu Glu Gln Glu Glu AspLeu Tyr Leu Asp Pro 50 55 60 gta caa cgc act gtt gcc gtg ttg ata gtc aattcc gat aaa gaa ggc 240 Val Gln Arg Thr Val Ala Val Leu Ile Val Asn SerAsp Lys Glu Gly 65 70 75 80 acg gga gaa aaa gaa aaa gta gaa gaa aat tcagat tgg gca gta tat 288 Thr Gly Glu Lys Glu Lys Val Glu Glu Asn Ser AspTrp Ala Val Tyr 85 90 95 ttc aac gag aaa gga gta cta aca gcc aga gaa atcacc ctc aaa gcc 336 Phe Asn Glu Lys Gly Val Leu Thr Ala Arg Glu Ile ThrLeu Lys Ala 100 105 110 ggc gac aac ctg aaa atc aaa caa aac ggc aca aacttc acc tac tcg 384 Gly Asp Asn Leu Lys Ile Lys Gln Asn Gly Thr Asn PheThr Tyr Ser 115 120 125 ctg aaa aaa gac ctc aca gat ctg acc agt gtt ggaact gaa aaa tta 432 Leu Lys Lys Asp Leu Thr Asp Leu Thr Ser Val Gly ThrGlu Lys Leu 130 135 140 tcg ttt agc gca aac ggc aat aaa gtc aac atc acaagc gac acc aaa 480 Ser Phe Ser Ala Asn Gly Asn Lys Val Asn Ile Thr SerAsp Thr Lys 145 150 155 160 ggc ttg aat ttt gcg aaa gaa acg gct ggg acgaac ggc gac acc acg 528 Gly Leu Asn Phe Ala Lys Glu Thr Ala Gly Thr AsnGly Asp Thr Thr 165 170 175 gtt cat ctg aac ggt att ggt tcg act ttg accgat acg ctg ctg aat 576 Val His Leu Asn Gly Ile Gly Ser Thr Leu Thr AspThr Leu Leu Asn 180 185 190 acc gga gcg acc aca aac gta acc aac gac aacgtt acc gat gac gag 624 Thr Gly Ala Thr Thr Asn Val Thr Asn Asp Asn ValThr Asp Asp Glu 195 200 205 aaa aaa cgt gcg gca agc gtt aaa gac gta ttaaac gct ggc tgg aac 672 Lys Lys Arg Ala Ala Ser Val Lys Asp Val Leu AsnAla Gly Trp Asn 210 215 220 att aaa ggc gtt aaa ccc ggt aca aca gct tccgat aac gtt gat ttc 720 Ile Lys Gly Val Lys Pro Gly Thr Thr Ala Ser AspAsn Val Asp Phe 225 230 235 240 gtc cgc act tac gac aca gtc gag ttc ttgagc gca gat acg aaa aca 768 Val Arg Thr Tyr Asp Thr Val Glu Phe Leu SerAla Asp Thr Lys Thr 245 250 255 acg act gtt aat gtg gaa agc aaa gac aacggc aag aaa acc gaa gtt 816 Thr Thr Val Asn Val Glu Ser Lys Asp Asn GlyLys Lys Thr Glu Val 260 265 270 aaa atc ggt gcg aag act tct gtt att aaagaa aaa gac ggt aag ttg 864 Lys Ile Gly Ala Lys Thr Ser Val Ile Lys GluLys Asp Gly Lys Leu 275 280 285 gtt act ggt aaa gac aaa ggc gag aat ggttct tct aca gac gaa ggc 912 Val Thr Gly Lys Asp Lys Gly Glu Asn Gly SerSer Thr Asp Glu Gly 290 295 300 gaa ggc tta gtg act gca aaa gaa gtg attgat gca gta aac aag gct 960 Glu Gly Leu Val Thr Ala Lys Glu Val Ile AspAla Val Asn Lys Ala 305 310 315 320 ggt tgg aga atg aaa aca aca acc gctaat ggt caa aca ggt caa gct 1008 Gly Trp Arg Met Lys Thr Thr Thr Ala AsnGly Gln Thr Gly Gln Ala 325 330 335 gac aag ttt gaa acc gtt aca tca ggcaca aat gta acc ttt gct agt 1056 Asp Lys Phe Glu Thr Val Thr Ser Gly ThrAsn Val Thr Phe Ala Ser 340 345 350 ggt aaa ggt aca act gcg act gta agtaaa gat gat caa ggc aac atc 1104 Gly Lys Gly Thr Thr Ala Thr Val Ser LysAsp Asp Gln Gly Asn Ile 355 360 365 act gtt atg tat gat gta aat gtc ggcgat gcc cta aac gtc aat cag 1152 Thr Val Met Tyr Asp Val Asn Val Gly AspAla Leu Asn Val Asn Gln 370 375 380 ctg caa aac agc ggt tgg aat ttg gattcc aaa gcg gtt gca ggt tct 1200 Leu Gln Asn Ser Gly Trp Asn Leu Asp SerLys Ala Val Ala Gly Ser 385 390 395 400 tcg ggc aaa gtc atc agc ggc aatgtt tcg ccg agc aag gga aag atg 1248 Ser Gly Lys Val Ile Ser Gly Asn ValSer Pro Ser Lys Gly Lys Met 405 410 415 gat gaa acc gtc aac att aat gccggc aac aac atc gag att acc cgc 1296 Asp Glu Thr Val Asn Ile Asn Ala GlyAsn Asn Ile Glu Ile Thr Arg 420 425 430 aac ggt aaa aat atc gac atc gccact tcg atg acc ccg cag ttt tcc 1344 Asn Gly Lys Asn Ile Asp Ile Ala ThrSer Met Thr Pro Gln Phe Ser 435 440 445 agc gtt tcg ctc ggc gcg ggg gcggat gcg ccc act ttg agc gtg gat 1392 Ser Val Ser Leu Gly Ala Gly Ala AspAla Pro Thr Leu Ser Val Asp 450 455 460 ggg gac gca ttg aat gtc ggc agcaag aag gac aac aaa ccc gtc cgc 1440 Gly Asp Ala Leu Asn Val Gly Ser LysLys Asp Asn Lys Pro Val Arg 465 470 475 480 att acc aat gtc gcc ccg ggcgtt aaa gag ggg gat gtt aca aac gtc 1488 Ile Thr Asn Val Ala Pro Gly ValLys Glu Gly Asp Val Thr Asn Val 485 490 495 gca caa ctt aaa ggc gtg gcgcaa aac ttg aac aac cgc atc gac aat 1536 Ala Gln Leu Lys Gly Val Ala GlnAsn Leu Asn Asn Arg Ile Asp Asn 500 505 510 gtg gac ggc aac gcg cgt gcgggc atc gcc caa gcg att gca acc gca 1584 Val Asp Gly Asn Ala Arg Ala GlyIle Ala Gln Ala Ile Ala Thr Ala 515 520 525 ggt ctg gtt cag gcg tat ttgccc ggc aag agt atg atg gcg atc ggc 1632 Gly Leu Val Gln Ala Tyr Leu ProGly Lys Ser Met Met Ala Ile Gly 530 535 540 ggc ggc act tat cgc ggc gaagcc ggt tac gcc atc ggc tac tcc agt 1680 Gly Gly Thr Tyr Arg Gly Glu AlaGly Tyr Ala Ile Gly Tyr Ser Ser 545 550 555 560 att tcc gac ggc gga aattgg att atc aaa ggc acg gct tcc ggc aat 1728 Ile Ser Asp Gly Gly Asn TrpIle Ile Lys Gly Thr Ala Ser Gly Asn 565 570 575 tcg cgc ggc cat ttc ggtgct tcc gca tct gtc ggt tat cag tgg 1773 Ser Arg Gly His Phe Gly Ala SerAla Ser Val Gly Tyr Gln Trp 580 585 590 12 591 PRT Neisseriameningitidis 12 Met Asn Lys Ile Tyr Arg Ile Ile Trp Asn Ser Ala Leu AsnAla Trp 1 5 10 15 Val Val Val Ser Glu Leu Thr Arg Asn His Thr Lys ArgAla Ser Ala 20 25 30 Thr Val Lys Thr Ala Val Leu Ala Thr Leu Leu Phe AlaThr Val Gln 35 40 45 Ala Ser Ala Asn Asn Glu Glu Gln Glu Glu Asp Leu TyrLeu Asp Pro 50 55 60 Val Gln Arg Thr Val Ala Val Leu Ile Val Asn Ser AspLys Glu Gly 65 70 75 80 Thr Gly Glu Lys Glu Lys Val Glu Glu Asn Ser AspTrp Ala Val Tyr 85 90 95 Phe Asn Glu Lys Gly Val Leu Thr Ala Arg Glu IleThr Leu Lys Ala 100 105 110 Gly Asp Asn Leu Lys Ile Lys Gln Asn Gly ThrAsn Phe Thr Tyr Ser 115 120 125 Leu Lys Lys Asp Leu Thr Asp Leu Thr SerVal Gly Thr Glu Lys Leu 130 135 140 Ser Phe Ser Ala Asn Gly Asn Lys ValAsn Ile Thr Ser Asp Thr Lys 145 150 155 160 Gly Leu Asn Phe Ala Lys GluThr Ala Gly Thr Asn Gly Asp Thr Thr 165 170 175 Val His Leu Asn Gly IleGly Ser Thr Leu Thr Asp Thr Leu Leu Asn 180 185 190 Thr Gly Ala Thr ThrAsn Val Thr Asn Asp Asn Val Thr Asp Asp Glu 195 200 205 Lys Lys Arg AlaAla Ser Val Lys Asp Val Leu Asn Ala Gly Trp Asn 210 215 220 Ile Lys GlyVal Lys Pro Gly Thr Thr Ala Ser Asp Asn Val Asp Phe 225 230 235 240 ValArg Thr Tyr Asp Thr Val Glu Phe Leu Ser Ala Asp Thr Lys Thr 245 250 255Thr Thr Val Asn Val Glu Ser Lys Asp Asn Gly Lys Lys Thr Glu Val 260 265270 Lys Ile Gly Ala Lys Thr Ser Val Ile Lys Glu Lys Asp Gly Lys Leu 275280 285 Val Thr Gly Lys Asp Lys Gly Glu Asn Gly Ser Ser Thr Asp Glu Gly290 295 300 Glu Gly Leu Val Thr Ala Lys Glu Val Ile Asp Ala Val Asn LysAla 305 310 315 320 Gly Trp Arg Met Lys Thr Thr Thr Ala Asn Gly Gln ThrGly Gln Ala 325 330 335 Asp Lys Phe Glu Thr Val Thr Ser Gly Thr Asn ValThr Phe Ala Ser 340 345 350 Gly Lys Gly Thr Thr Ala Thr Val Ser Lys AspAsp Gln Gly Asn Ile 355 360 365 Thr Val Met Tyr Asp Val Asn Val Gly AspAla Leu Asn Val Asn Gln 370 375 380 Leu Gln Asn Ser Gly Trp Asn Leu AspSer Lys Ala Val Ala Gly Ser 385 390 395 400 Ser Gly Lys Val Ile Ser GlyAsn Val Ser Pro Ser Lys Gly Lys Met 405 410 415 Asp Glu Thr Val Asn IleAsn Ala Gly Asn Asn Ile Glu Ile Thr Arg 420 425 430 Asn Gly Lys Asn IleAsp Ile Ala Thr Ser Met Thr Pro Gln Phe Ser 435 440 445 Ser Val Ser LeuGly Ala Gly Ala Asp Ala Pro Thr Leu Ser Val Asp 450 455 460 Gly Asp AlaLeu Asn Val Gly Ser Lys Lys Asp Asn Lys Pro Val Arg 465 470 475 480 IleThr Asn Val Ala Pro Gly Val Lys Glu Gly Asp Val Thr Asn Val 485 490 495Ala Gln Leu Lys Gly Val Ala Gln Asn Leu Asn Asn Arg Ile Asp Asn 500 505510 Val Asp Gly Asn Ala Arg Ala Gly Ile Ala Gln Ala Ile Ala Thr Ala 515520 525 Gly Leu Val Gln Ala Tyr Leu Pro Gly Lys Ser Met Met Ala Ile Gly530 535 540 Gly Gly Thr Tyr Arg Gly Glu Ala Gly Tyr Ala Ile Gly Tyr SerSer 545 550 555 560 Ile Ser Asp Gly Gly Asn Trp Ile Ile Lys Gly Thr AlaSer Gly Asn 565 570 575 Ser Arg Gly His Phe Gly Ala Ser Ala Ser Val GlyTyr Gln Trp 580 585 590 13 549 DNA Neisseria meningitidis CDS (1)..(549)NMA0174 13 atg aaa gtt ttg aac ggt tgg tcg gac agg aag atg tgg cgg gttttg 48 Met Lys Val Leu Asn Gly Trp Ser Asp Arg Lys Met Trp Arg Val Leu 15 10 15 agt gct ttg ccg ata ggc gtg gtg ttt ttt gat ttg atc tac ggt ttt96 Ser Ala Leu Pro Ile Gly Val Val Phe Phe Asp Leu Ile Tyr Gly Phe 20 2530 gtg ttg aat gtg ttg cag ggt ttg gat ttg cag cgt gcc gtg ccg gat 144Val Leu Asn Val Leu Gln Gly Leu Asp Leu Gln Arg Ala Val Pro Asp 35 40 45tcg gaa ggc gtg ttg gcg gtt acg ccc gat att gca ttc aac agt ttg 192 SerGlu Gly Val Leu Ala Val Thr Pro Asp Ile Ala Phe Asn Ser Leu 50 55 60 cagatt gtc gcc aac ggc ggt atg gcg gcg gtg gtc tgt ttc ggg ttg 240 Gln IleVal Ala Asn Gly Gly Met Ala Ala Val Val Cys Phe Gly Leu 65 70 75 80 gcggtt gtg ttt ttg ctc aac cgt tcg gtg cgg cgg cgg cag gtg ttg 288 Ala ValVal Phe Leu Leu Asn Arg Ser Val Arg Arg Arg Gln Val Leu 85 90 95 gaa atcggg gtg ttc cgg atg ttg ggg ctg gtg gcg gta ttg gcg ttc 336 Glu Ile GlyVal Phe Arg Met Leu Gly Leu Val Ala Val Leu Ala Phe 100 105 110 agc gcgccg tcg gtg tgg gag tgg gcg aac gcg ctg ccg ctg ctg ctg 384 Ser Ala ProSer Val Trp Glu Trp Ala Asn Ala Leu Pro Leu Leu Leu 115 120 125 aag ggcgcg gac gtg gtc aat acg ggg aat gcg cgt tat gtg ctg acg 432 Lys Gly AlaAsp Val Val Asn Thr Gly Asn Ala Arg Tyr Val Leu Thr 130 135 140 gct ttgtgt atg ccc ttt cct gcg gtg tcg tgc gtc atc ggg ctg gtg 480 Ala Leu CysMet Pro Phe Pro Ala Val Ser Cys Val Ile Gly Leu Val 145 150 155 160 gggcgg ttc agg ctt cag acg gca tcg ggc agg gcg gca aag tca ggg 528 Gly ArgPhe Arg Leu Gln Thr Ala Ser Gly Arg Ala Ala Lys Ser Gly 165 170 175 ggtgcg ggc aag gcg gac gga 549 Gly Ala Gly Lys Ala Asp Gly 180 14 183 PRTNeisseria meningitidis 14 Met Lys Val Leu Asn Gly Trp Ser Asp Arg LysMet Trp Arg Val Leu 1 5 10 15 Ser Ala Leu Pro Ile Gly Val Val Phe PheAsp Leu Ile Tyr Gly Phe 20 25 30 Val Leu Asn Val Leu Gln Gly Leu Asp LeuGln Arg Ala Val Pro Asp 35 40 45 Ser Glu Gly Val Leu Ala Val Thr Pro AspIle Ala Phe Asn Ser Leu 50 55 60 Gln Ile Val Ala Asn Gly Gly Met Ala AlaVal Val Cys Phe Gly Leu 65 70 75 80 Ala Val Val Phe Leu Leu Asn Arg SerVal Arg Arg Arg Gln Val Leu 85 90 95 Glu Ile Gly Val Phe Arg Met Leu GlyLeu Val Ala Val Leu Ala Phe 100 105 110 Ser Ala Pro Ser Val Trp Glu TrpAla Asn Ala Leu Pro Leu Leu Leu 115 120 125 Lys Gly Ala Asp Val Val AsnThr Gly Asn Ala Arg Tyr Val Leu Thr 130 135 140 Ala Leu Cys Met Pro PhePro Ala Val Ser Cys Val Ile Gly Leu Val 145 150 155 160 Gly Arg Phe ArgLeu Gln Thr Ala Ser Gly Arg Ala Ala Lys Ser Gly 165 170 175 Gly Ala GlyLys Ala Asp Gly 180 15 2373 DNA Neisseria meningitidis CDS (1)..(2373)NMA1371 15 atg aat aaa aat att aaa tct tta aat tta cgg gaa aaa gac ccgttt 48 Met Asn Lys Asn Ile Lys Ser Leu Asn Leu Arg Glu Lys Asp Pro Phe 15 10 15 tta agt cgt gaa aaa cag cgt tat gaa cat cct ttg ccc agt cgg gaa96 Leu Ser Arg Glu Lys Gln Arg Tyr Glu His Pro Leu Pro Ser Arg Glu 20 2530 tgg ata atc gag ctg ctt gaa cgt aaa ggc gta cca tcc aag att gaa 144Trp Ile Ile Glu Leu Leu Glu Arg Lys Gly Val Pro Ser Lys Ile Glu 35 40 45gct ttg gta cgc gaa ttg tcg att aag gaa gaa gag tac gaa ttt ttc 192 AlaLeu Val Arg Glu Leu Ser Ile Lys Glu Glu Glu Tyr Glu Phe Phe 50 55 60 gaacgt cgt ctg aag gcg atg gcg cgg gac ggt cag gtt tta atc aac 240 Glu ArgArg Leu Lys Ala Met Ala Arg Asp Gly Gln Val Leu Ile Asn 65 70 75 80 cgtcgg ggc gcg gtt tgc gcg gcg gac aaa ttg gat ttg gtc aaa tgc 288 Arg ArgGly Ala Val Cys Ala Ala Asp Lys Leu Asp Leu Val Lys Cys 85 90 95 cgt gtcgag gcg cac aaa gac ggc ttc ggt ttc gcc gtg ccg ctc acg 336 Arg Val GluAla His Lys Asp Gly Phe Gly Phe Ala Val Pro Leu Thr 100 105 110 ccc gccaaa gac ggt gat ttt gtt ttg tac gaa cgc cag atg cgc ggc 384 Pro Ala LysAsp Gly Asp Phe Val Leu Tyr Glu Arg Gln Met Arg Gly 115 120 125 att atgcac ggc gat att gtc act gtt cgt cct gcc ggc atg gac cgt 432 Ile Met HisGly Asp Ile Val Thr Val Arg Pro Ala Gly Met Asp Arg 130 135 140 agg ggccgc cgc gaa ggg acg gtt ctg gat att gtc gaa cgc gcg caa 480 Arg Gly ArgArg Glu Gly Thr Val Leu Asp Ile Val Glu Arg Ala Gln 145 150 155 160 agcaaa gtg gtc ggc cgt ttc tat atg gat agg ggc gtg gcg att ttg 528 Ser LysVal Val Gly Arg Phe Tyr Met Asp Arg Gly Val Ala Ile Leu 165 170 175 gagccg gaa gac aag cgt ctg aac caa agc atc gta ttg gaa ccg gac 576 Glu ProGlu Asp Lys Arg Leu Asn Gln Ser Ile Val Leu Glu Pro Asp 180 185 190 ggcgtg gcg cgt ttc aaa cct gaa tcc ggt cag gtc atc gtc ggc gaa 624 Gly ValAla Arg Phe Lys Pro Glu Ser Gly Gln Val Ile Val Gly Glu 195 200 205 attgag gtt tat cct gag caa aac cgg ccg gca gtg gca aaa atc atc 672 Ile GluVal Tyr Pro Glu Gln Asn Arg Pro Ala Val Ala Lys Ile Ile 210 215 220 gaagtt ttg ggc gat tat gcc gac agc ggc atg gag att gaa att gcc 720 Glu ValLeu Gly Asp Tyr Ala Asp Ser Gly Met Glu Ile Glu Ile Ala 225 230 235 240gtg cgc aag cat cat ttg ccg cac caa ttc agt gaa gcg tgt gcc aaa 768 ValArg Lys His His Leu Pro His Gln Phe Ser Glu Ala Cys Ala Lys 245 250 255gcc gcg aaa aaa att ccc gac cat gta cgc aaa agc gat ttg aaa ggc 816 AlaAla Lys Lys Ile Pro Asp His Val Arg Lys Ser Asp Leu Lys Gly 260 265 270cgc gtc gat ttg cgc gac ctg cct ttg gta acg ata gac ggc gaa acg 864 ArgVal Asp Leu Arg Asp Leu Pro Leu Val Thr Ile Asp Gly Glu Thr 275 280 285gct cga gat ttt gac gat gcg gtg ttt gcc gag aaa atc gga cgc aat 912 AlaArg Asp Phe Asp Asp Ala Val Phe Ala Glu Lys Ile Gly Arg Asn 290 295 300tac cgt ctg gtc gtg gcg att gcc gat gtc agc cat tat gtc cgc ccc 960 TyrArg Leu Val Val Ala Ile Ala Asp Val Ser His Tyr Val Arg Pro 305 310 315320 gat gac gct atc gac acg gac gct cag gaa cgc agc acc agt gtt tac 1008Asp Asp Ala Ile Asp Thr Asp Ala Gln Glu Arg Ser Thr Ser Val Tyr 325 330335 ttc ccg cgc cgc gtg att ccc atg ttg ccg gaa aac ctg tcc aac ggc 1056Phe Pro Arg Arg Val Ile Pro Met Leu Pro Glu Asn Leu Ser Asn Gly 340 345350 atc tgc tcg ctc aat cct cat gtc gag cgt ttg tgt gtg gtg tgc gat 1104Ile Cys Ser Leu Asn Pro His Val Glu Arg Leu Cys Val Val Cys Asp 355 360365 atg gtt atc act tac gcg ggc aat atc aaa gaa tac cgc ttc tac ccc 1152Met Val Ile Thr Tyr Ala Gly Asn Ile Lys Glu Tyr Arg Phe Tyr Pro 370 375380 gcc gtg atg cgc tct cat gcc cgc ctg acc tac aac caa gtt tgg aaa 1200Ala Val Met Arg Ser His Ala Arg Leu Thr Tyr Asn Gln Val Trp Lys 385 390395 400 tgg ctt tca ggc ggc atc gag cat ccg ttc aaa acc caa atc gac acg1248 Trp Leu Ser Gly Gly Ile Glu His Pro Phe Lys Thr Gln Ile Asp Thr 405410 415 ctt tac aaa ctc ttc aaa atc ctt cag aaa aag cgt ttc gaa cgc ggg1296 Leu Tyr Lys Leu Phe Lys Ile Leu Gln Lys Lys Arg Phe Glu Arg Gly 420425 430 gcg gtg gag ttt gac agc atc gaa acc caa atg ctt ttc gac gac aac1344 Ala Val Glu Phe Asp Ser Ile Glu Thr Gln Met Leu Phe Asp Asp Asn 435440 445 ggt aaa att gaa aaa atc gtc ccc gtt gtc cgc aac gat gcc cac aag1392 Gly Lys Ile Glu Lys Ile Val Pro Val Val Arg Asn Asp Ala His Lys 450455 460 ctg att gaa gaa tgt atg ttg gcg gca aac gtt tgc gca gcg gat ttt1440 Leu Ile Glu Glu Cys Met Leu Ala Ala Asn Val Cys Ala Ala Asp Phe 465470 475 480 ctg ttg aaa aac aag cat acc gca ttg ttc cgc aac cat ttg gggccc 1488 Leu Leu Lys Asn Lys His Thr Ala Leu Phe Arg Asn His Leu Gly Pro485 490 495 acg ccc gaa aaa ctc gcc gcc ttg cgc gag cag ctc ggt ctg ttgggg 1536 Thr Pro Glu Lys Leu Ala Ala Leu Arg Glu Gln Leu Gly Leu Leu Gly500 505 510 ctt caa ctt ggc ggc ggc gac aac ccg tcg ccg aaa gac tat gccgcg 1584 Leu Gln Leu Gly Gly Gly Asp Asn Pro Ser Pro Lys Asp Tyr Ala Ala515 520 525 ctt gcc gga cag ttc aaa ggc agg ccg gat gcc gaa ttg ctg caagtc 1632 Leu Ala Gly Gln Phe Lys Gly Arg Pro Asp Ala Glu Leu Leu Gln Val530 535 540 atg atg ttg cgc tcc atg caa cag gcg gtt tac gaa ccg cat tgcgac 1680 Met Met Leu Arg Ser Met Gln Gln Ala Val Tyr Glu Pro His Cys Asp545 550 555 560 gga cac ttt ggt ctt gcc tac gaa gca tac gcc cac ttc acctcg ccc 1728 Gly His Phe Gly Leu Ala Tyr Glu Ala Tyr Ala His Phe Thr SerPro 565 570 575 atc cgc cgc tat ccc gac ctg acc gta cac cgc gcc atc aaagcc gtg 1776 Ile Arg Arg Tyr Pro Asp Leu Thr Val His Arg Ala Ile Lys AlaVal 580 585 590 ttg aat cag caa acc tac acg cca aaa aaa agc tgg cag gctttg ggc 1824 Leu Asn Gln Gln Thr Tyr Thr Pro Lys Lys Ser Trp Gln Ala LeuGly 595 600 605 gtg cat acc tcg ttc tgt gag cgc cgt gcc gac gac gcc agccgc gac 1872 Val His Thr Ser Phe Cys Glu Arg Arg Ala Asp Asp Ala Ser ArgAsp 610 615 620 gtg gaa aac tgg ctg aaa acc tat tat atg cgc gat aag gtcggc gaa 1920 Val Glu Asn Trp Leu Lys Thr Tyr Tyr Met Arg Asp Lys Val GlyGlu 625 630 635 640 gta ttc gaa ggt aaa atc tcc ggc atg acc agt ttt ggtatc ttt gta 1968 Val Phe Glu Gly Lys Ile Ser Gly Met Thr Ser Phe Gly IlePhe Val 645 650 655 aca ctg gac ggc atc cac att gac ggc ttg gtg cat atcagc gat ttg 2016 Thr Leu Asp Gly Ile His Ile Asp Gly Leu Val His Ile SerAsp Leu 660 665 670 ggc gaa gac tat ttc aac ttc cgc ccc gaa atc atg gcaatc gaa ggc 2064 Gly Glu Asp Tyr Phe Asn Phe Arg Pro Glu Ile Met Ala IleGlu Gly 675 680 685 gaa cgc agc ggc atc cgt ttc aac atg ggg gac agg gttgcc gtc cgg 2112 Glu Arg Ser Gly Ile Arg Phe Asn Met Gly Asp Arg Val AlaVal Arg 690 695 700 gtc gcc cgt gcc gat ttg gat gac gga aaa atc gat tttgtc ctg att 2160 Val Ala Arg Ala Asp Leu Asp Asp Gly Lys Ile Asp Phe ValLeu Ile 705 710 715 720 gcc ggg ggg agc ggc agg ggg cgg aaa gtt aaa tcatcc gcg tct gcc 2208 Ala Gly Gly Ser Gly Arg Gly Arg Lys Val Lys Ser SerAla Ser Ala 725 730 735 aaa ccg gca ggg acg gcg ggg aaa ggg aag ccg aaaacc gcc gcc gag 2256 Lys Pro Ala Gly Thr Ala Gly Lys Gly Lys Pro Lys ThrAla Ala Glu 740 745 750 aaa aaa aca gcc cga ggc ggc aaa gta agg gga aggggc gcg tct gcc 2304 Lys Lys Thr Ala Arg Gly Gly Lys Val Arg Gly Arg GlyAla Ser Ala 755 760 765 gcc gca gaa tcg agg aaa aag gca aag aaa ccg gttccg att aag gta 2352 Ala Ala Glu Ser Arg Lys Lys Ala Lys Lys Pro Val ProIle Lys Val 770 775 780 aaa aaa cgg aaa ggc aaa tca 2373 Lys Lys Arg LysGly Lys Ser 785 790 16 791 PRT Neisseria meningitidis 16 Met Asn Lys AsnIle Lys Ser Leu Asn Leu Arg Glu Lys Asp Pro Phe 1 5 10 15 Leu Ser ArgGlu Lys Gln Arg Tyr Glu His Pro Leu Pro Ser Arg Glu 20 25 30 Trp Ile IleGlu Leu Leu Glu Arg Lys Gly Val Pro Ser Lys Ile Glu 35 40 45 Ala Leu ValArg Glu Leu Ser Ile Lys Glu Glu Glu Tyr Glu Phe Phe 50 55 60 Glu Arg ArgLeu Lys Ala Met Ala Arg Asp Gly Gln Val Leu Ile Asn 65 70 75 80 Arg ArgGly Ala Val Cys Ala Ala Asp Lys Leu Asp Leu Val Lys Cys 85 90 95 Arg ValGlu Ala His Lys Asp Gly Phe Gly Phe Ala Val Pro Leu Thr 100 105 110 ProAla Lys Asp Gly Asp Phe Val Leu Tyr Glu Arg Gln Met Arg Gly 115 120 125Ile Met His Gly Asp Ile Val Thr Val Arg Pro Ala Gly Met Asp Arg 130 135140 Arg Gly Arg Arg Glu Gly Thr Val Leu Asp Ile Val Glu Arg Ala Gln 145150 155 160 Ser Lys Val Val Gly Arg Phe Tyr Met Asp Arg Gly Val Ala IleLeu 165 170 175 Glu Pro Glu Asp Lys Arg Leu Asn Gln Ser Ile Val Leu GluPro Asp 180 185 190 Gly Val Ala Arg Phe Lys Pro Glu Ser Gly Gln Val IleVal Gly Glu 195 200 205 Ile Glu Val Tyr Pro Glu Gln Asn Arg Pro Ala ValAla Lys Ile Ile 210 215 220 Glu Val Leu Gly Asp Tyr Ala Asp Ser Gly MetGlu Ile Glu Ile Ala 225 230 235 240 Val Arg Lys His His Leu Pro His GlnPhe Ser Glu Ala Cys Ala Lys 245 250 255 Ala Ala Lys Lys Ile Pro Asp HisVal Arg Lys Ser Asp Leu Lys Gly 260 265 270 Arg Val Asp Leu Arg Asp LeuPro Leu Val Thr Ile Asp Gly Glu Thr 275 280 285 Ala Arg Asp Phe Asp AspAla Val Phe Ala Glu Lys Ile Gly Arg Asn 290 295 300 Tyr Arg Leu Val ValAla Ile Ala Asp Val Ser His Tyr Val Arg Pro 305 310 315 320 Asp Asp AlaIle Asp Thr Asp Ala Gln Glu Arg Ser Thr Ser Val Tyr 325 330 335 Phe ProArg Arg Val Ile Pro Met Leu Pro Glu Asn Leu Ser Asn Gly 340 345 350 IleCys Ser Leu Asn Pro His Val Glu Arg Leu Cys Val Val Cys Asp 355 360 365Met Val Ile Thr Tyr Ala Gly Asn Ile Lys Glu Tyr Arg Phe Tyr Pro 370 375380 Ala Val Met Arg Ser His Ala Arg Leu Thr Tyr Asn Gln Val Trp Lys 385390 395 400 Trp Leu Ser Gly Gly Ile Glu His Pro Phe Lys Thr Gln Ile AspThr 405 410 415 Leu Tyr Lys Leu Phe Lys Ile Leu Gln Lys Lys Arg Phe GluArg Gly 420 425 430 Ala Val Glu Phe Asp Ser Ile Glu Thr Gln Met Leu PheAsp Asp Asn 435 440 445 Gly Lys Ile Glu Lys Ile Val Pro Val Val Arg AsnAsp Ala His Lys 450 455 460 Leu Ile Glu Glu Cys Met Leu Ala Ala Asn ValCys Ala Ala Asp Phe 465 470 475 480 Leu Leu Lys Asn Lys His Thr Ala LeuPhe Arg Asn His Leu Gly Pro 485 490 495 Thr Pro Glu Lys Leu Ala Ala LeuArg Glu Gln Leu Gly Leu Leu Gly 500 505 510 Leu Gln Leu Gly Gly Gly AspAsn Pro Ser Pro Lys Asp Tyr Ala Ala 515 520 525 Leu Ala Gly Gln Phe LysGly Arg Pro Asp Ala Glu Leu Leu Gln Val 530 535 540 Met Met Leu Arg SerMet Gln Gln Ala Val Tyr Glu Pro His Cys Asp 545 550 555 560 Gly His PheGly Leu Ala Tyr Glu Ala Tyr Ala His Phe Thr Ser Pro 565 570 575 Ile ArgArg Tyr Pro Asp Leu Thr Val His Arg Ala Ile Lys Ala Val 580 585 590 LeuAsn Gln Gln Thr Tyr Thr Pro Lys Lys Ser Trp Gln Ala Leu Gly 595 600 605Val His Thr Ser Phe Cys Glu Arg Arg Ala Asp Asp Ala Ser Arg Asp 610 615620 Val Glu Asn Trp Leu Lys Thr Tyr Tyr Met Arg Asp Lys Val Gly Glu 625630 635 640 Val Phe Glu Gly Lys Ile Ser Gly Met Thr Ser Phe Gly Ile PheVal 645 650 655 Thr Leu Asp Gly Ile His Ile Asp Gly Leu Val His Ile SerAsp Leu 660 665 670 Gly Glu Asp Tyr Phe Asn Phe Arg Pro Glu Ile Met AlaIle Glu Gly 675 680 685 Glu Arg Ser Gly Ile Arg Phe Asn Met Gly Asp ArgVal Ala Val Arg 690 695 700 Val Ala Arg Ala Asp Leu Asp Asp Gly Lys IleAsp Phe Val Leu Ile 705 710 715 720 Ala Gly Gly Ser Gly Arg Gly Arg LysVal Lys Ser Ser Ala Ser Ala 725 730 735 Lys Pro Ala Gly Thr Ala Gly LysGly Lys Pro Lys Thr Ala Ala Glu 740 745 750 Lys Lys Thr Ala Arg Gly GlyLys Val Arg Gly Arg Gly Ala Ser Ala 755 760 765 Ala Ala Glu Ser Arg LysLys Ala Lys Lys Pro Val Pro Ile Lys Val 770 775 780 Lys Lys Arg Lys GlyLys Ser 785 790 17 1605 DNA Neisseria meningitidis CDS (1)..(1605)NMA1555 17 atg agt atc gta gaa atc aaa gtc ccc gat atc ggc ggt cac gaaaac 48 Met Ser Ile Val Glu Ile Lys Val Pro Asp Ile Gly Gly His Glu Asn 15 10 15 gtc gac atc atc gcc gta gaa gtt aaa gcg ggc gac acc atc gcc gtt96 Val Asp Ile Ile Ala Val Glu Val Lys Ala Gly Asp Thr Ile Ala Val 20 2530 gac gac acc ctg att aca ctg gaa acc gac aaa gcc acg atg gat gtg 144Asp Asp Thr Leu Ile Thr Leu Glu Thr Asp Lys Ala Thr Met Asp Val 35 40 45cct gcc gat gcg gcc ggt gtc gtg aaa gaa gtg aaa gtc aaa gtc ggc 192 ProAla Asp Ala Ala Gly Val Val Lys Glu Val Lys Val Lys Val Gly 50 55 60 gacaaa atc tcc gaa ggc ggc gta att ctg acc gtt gaa acc ggt gcc 240 Asp LysIle Ser Glu Gly Gly Val Ile Leu Thr Val Glu Thr Gly Ala 65 70 75 80 gccgcc gcc gaa gcc gcc ccg gct gct gcc gaa gca caa cct gca cct 288 Ala AlaAla Glu Ala Ala Pro Ala Ala Ala Glu Ala Gln Pro Ala Pro 85 90 95 gct gccgca ccc gct gcc gca ggc ggt gca acc gtt caa gta gcc gtt 336 Ala Ala AlaPro Ala Ala Ala Gly Gly Ala Thr Val Gln Val Ala Val 100 105 110 ccc gatatc ggc ggc cat acc gat gtg gat gta atc gcc gtt gaa atc 384 Pro Asp IleGly Gly His Thr Asp Val Asp Val Ile Ala Val Glu Ile 115 120 125 aaa gtgggc gac acc gtt gcc gaa gac gac acg ctg att act ttg gaa 432 Lys Val GlyAsp Thr Val Ala Glu Asp Asp Thr Leu Ile Thr Leu Glu 130 135 140 acc gataaa gcg aca atg gac gta cct tgt acc gct gcc ggt gtc gtt 480 Thr Asp LysAla Thr Met Asp Val Pro Cys Thr Ala Ala Gly Val Val 145 150 155 160 aaagcc gta ttc tta aaa gtc ggc gac aaa gta tcc gaa ggc tct gcc 528 Lys AlaVal Phe Leu Lys Val Gly Asp Lys Val Ser Glu Gly Ser Ala 165 170 175 attatc gaa gta gaa acc gtc ggc tct gcc gca gca gcc cct gct caa 576 Ile IleGlu Val Glu Thr Val Gly Ser Ala Ala Ala Ala Pro Ala Gln 180 185 190 gccgct caa gct gcc gca ccg gct gcc gct ccg cct ccg act gct gcc 624 Ala AlaGln Ala Ala Ala Pro Ala Ala Ala Pro Pro Pro Thr Ala Ala 195 200 205 gccgca ccc gcc gcc gcg cct gca cct tct gca cct gcc gct gcc aaa 672 Ala AlaPro Ala Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala Ala Lys 210 215 220 atcgac gag gcc gct ttc gcc aaa gca cac gcc ggc cct tcc gca cgc 720 Ile AspGlu Ala Ala Phe Ala Lys Ala His Ala Gly Pro Ser Ala Arg 225 230 235 240aaa ctg gcg cgc gaa ttg ggc gtg gat ttg ggc caa gtc aaa ggt acc 768 LysLeu Ala Arg Glu Leu Gly Val Asp Leu Gly Gln Val Lys Gly Thr 245 250 255ggc ttg aaa ggc cgc atc gtg ggc gac gac atc aaa gcc ttt gtg aaa 816 GlyLeu Lys Gly Arg Ile Val Gly Asp Asp Ile Lys Ala Phe Val Lys 260 265 270tcc gta atg cag ggc ggc gcg gca aaa cct gcc gca gtc ggc gcg tct 864 SerVal Met Gln Gly Gly Ala Ala Lys Pro Ala Ala Val Gly Ala Ser 275 280 285tta ggc ggc ggt ctg gac ttg ctg ccc tgg cct aaa gtg gac ttc tcc 912 LeuGly Gly Gly Leu Asp Leu Leu Pro Trp Pro Lys Val Asp Phe Ser 290 295 300aaa ttc ggt aat gtc gaa gtt aaa gaa ttg tcc cgc att aag aaa att 960 LysPhe Gly Asn Val Glu Val Lys Glu Leu Ser Arg Ile Lys Lys Ile 305 310 315320 tcc ggt caa aac ctg tct cgc aac tgg gtg gtg att ccc cac gtt acc 1008Ser Gly Gln Asn Leu Ser Arg Asn Trp Val Val Ile Pro His Val Thr 325 330335 gta cac gaa gaa gca gat atg acc gag ctg gaa gaa ttc cgc aaa caa 1056Val His Glu Glu Ala Asp Met Thr Glu Leu Glu Glu Phe Arg Lys Gln 340 345350 ctg aac aaa gaa tgg gaa cgc gaa ggc gtg aaa ctg tca ccg ttg gcg 1104Leu Asn Lys Glu Trp Glu Arg Glu Gly Val Lys Leu Ser Pro Leu Ala 355 360365 ttc atc atc aaa gcc tcc gtt tcc gcg ctg aaa gcc ttc ccc gaa ttc 1152Phe Ile Ile Lys Ala Ser Val Ser Ala Leu Lys Ala Phe Pro Glu Phe 370 375380 aac gct tct ctg gac ggc gac aac ttg gtg ctg aaa aac tac ttc aac 1200Asn Ala Ser Leu Asp Gly Asp Asn Leu Val Leu Lys Asn Tyr Phe Asn 385 390395 400 atc ggt ttc gca gca gac acg ccg aac ggc ttg gtt gtt cca gta atc1248 Ile Gly Phe Ala Ala Asp Thr Pro Asn Gly Leu Val Val Pro Val Ile 405410 415 aaa gac gtg gat caa aaa ggc ttg aaa caa atc agc caa gag ctg acc1296 Lys Asp Val Asp Gln Lys Gly Leu Lys Gln Ile Ser Gln Glu Leu Thr 420425 430 gaa ttg tct aaa aaa gcc cgc gaa ggc aag ctc aaa cca caa gaa atg1344 Glu Leu Ser Lys Lys Ala Arg Glu Gly Lys Leu Lys Pro Gln Glu Met 435440 445 caa ggc gca tgc ttt acc att tcc agc ttg ggc ggc atc ggc ggc aca1392 Gln Gly Ala Cys Phe Thr Ile Ser Ser Leu Gly Gly Ile Gly Gly Thr 450455 460 ggt ttc acg ccg att gtg aac gct ccc gaa gtc gcc atc ttg ggc gtg1440 Gly Phe Thr Pro Ile Val Asn Ala Pro Glu Val Ala Ile Leu Gly Val 465470 475 480 tgc aaa tcc caa atc aaa ccg gtt tgg aac ggc aaa gaa ttc gctcct 1488 Cys Lys Ser Gln Ile Lys Pro Val Trp Asn Gly Lys Glu Phe Ala Pro485 490 495 cgc ctg atg tgc cca ttg agc ctg tcc ttt gac cac cgt gtc atcgac 1536 Arg Leu Met Cys Pro Leu Ser Leu Ser Phe Asp His Arg Val Ile Asp500 505 510 ggc gcg gcc ggt atg cgc ttc acc gta ttc ctg gcc aac ctg ttgaaa 1584 Gly Ala Ala Gly Met Arg Phe Thr Val Phe Leu Ala Asn Leu Leu Lys515 520 525 gac ttc cgc cgc att act ctg 1605 Asp Phe Arg Arg Ile Thr Leu530 535 18 535 PRT Neisseria meningitidis 18 Met Ser Ile Val Glu Ile LysVal Pro Asp Ile Gly Gly His Glu Asn 1 5 10 15 Val Asp Ile Ile Ala ValGlu Val Lys Ala Gly Asp Thr Ile Ala Val 20 25 30 Asp Asp Thr Leu Ile ThrLeu Glu Thr Asp Lys Ala Thr Met Asp Val 35 40 45 Pro Ala Asp Ala Ala GlyVal Val Lys Glu Val Lys Val Lys Val Gly 50 55 60 Asp Lys Ile Ser Glu GlyGly Val Ile Leu Thr Val Glu Thr Gly Ala 65 70 75 80 Ala Ala Ala Glu AlaAla Pro Ala Ala Ala Glu Ala Gln Pro Ala Pro 85 90 95 Ala Ala Ala Pro AlaAla Ala Gly Gly Ala Thr Val Gln Val Ala Val 100 105 110 Pro Asp Ile GlyGly His Thr Asp Val Asp Val Ile Ala Val Glu Ile 115 120 125 Lys Val GlyAsp Thr Val Ala Glu Asp Asp Thr Leu Ile Thr Leu Glu 130 135 140 Thr AspLys Ala Thr Met Asp Val Pro Cys Thr Ala Ala Gly Val Val 145 150 155 160Lys Ala Val Phe Leu Lys Val Gly Asp Lys Val Ser Glu Gly Ser Ala 165 170175 Ile Ile Glu Val Glu Thr Val Gly Ser Ala Ala Ala Ala Pro Ala Gln 180185 190 Ala Ala Gln Ala Ala Ala Pro Ala Ala Ala Pro Pro Pro Thr Ala Ala195 200 205 Ala Ala Pro Ala Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala AlaLys 210 215 220 Ile Asp Glu Ala Ala Phe Ala Lys Ala His Ala Gly Pro SerAla Arg 225 230 235 240 Lys Leu Ala Arg Glu Leu Gly Val Asp Leu Gly GlnVal Lys Gly Thr 245 250 255 Gly Leu Lys Gly Arg Ile Val Gly Asp Asp IleLys Ala Phe Val Lys 260 265 270 Ser Val Met Gln Gly Gly Ala Ala Lys ProAla Ala Val Gly Ala Ser 275 280 285 Leu Gly Gly Gly Leu Asp Leu Leu ProTrp Pro Lys Val Asp Phe Ser 290 295 300 Lys Phe Gly Asn Val Glu Val LysGlu Leu Ser Arg Ile Lys Lys Ile 305 310 315 320 Ser Gly Gln Asn Leu SerArg Asn Trp Val Val Ile Pro His Val Thr 325 330 335 Val His Glu Glu AlaAsp Met Thr Glu Leu Glu Glu Phe Arg Lys Gln 340 345 350 Leu Asn Lys GluTrp Glu Arg Glu Gly Val Lys Leu Ser Pro Leu Ala 355 360 365 Phe Ile IleLys Ala Ser Val Ser Ala Leu Lys Ala Phe Pro Glu Phe 370 375 380 Asn AlaSer Leu Asp Gly Asp Asn Leu Val Leu Lys Asn Tyr Phe Asn 385 390 395 400Ile Gly Phe Ala Ala Asp Thr Pro Asn Gly Leu Val Val Pro Val Ile 405 410415 Lys Asp Val Asp Gln Lys Gly Leu Lys Gln Ile Ser Gln Glu Leu Thr 420425 430 Glu Leu Ser Lys Lys Ala Arg Glu Gly Lys Leu Lys Pro Gln Glu Met435 440 445 Gln Gly Ala Cys Phe Thr Ile Ser Ser Leu Gly Gly Ile Gly GlyThr 450 455 460 Gly Phe Thr Pro Ile Val Asn Ala Pro Glu Val Ala Ile LeuGly Val 465 470 475 480 Cys Lys Ser Gln Ile Lys Pro Val Trp Asn Gly LysGlu Phe Ala Pro 485 490 495 Arg Leu Met Cys Pro Leu Ser Leu Ser Phe AspHis Arg Val Ile Asp 500 505 510 Gly Ala Ala Gly Met Arg Phe Thr Val PheLeu Ala Asn Leu Leu Lys 515 520 525 Asp Phe Arg Arg Ile Thr Leu 530 53519 321 DNA Neisseria meningitidis CDS (1)..(321) NMA0039 19 atg ttt aaggac gaa cta aat gaa ttt atc cga ttg ata tcc gat ccg 48 Met Phe Lys AspGlu Leu Asn Glu Phe Ile Arg Leu Ile Ser Asp Pro 1 5 10 15 gaa agt gaattg gat gaa tgg tat ttg agt gat ttt aaa gat gaa cat 96 Glu Ser Glu LeuAsp Glu Trp Tyr Leu Ser Asp Phe Lys Asp Glu His 20 25 30 ata tgg gaa atgcaa agt tac gaa gca ttt tca tgt ttg cgg gaa gca 144 Ile Trp Glu Met GlnSer Tyr Glu Ala Phe Ser Cys Leu Arg Glu Ala 35 40 45 gtc ccc tat ttg tttgct tat ccc cga tac ggg tat gaa ctg ttg gag 192 Val Pro Tyr Leu Phe AlaTyr Pro Arg Tyr Gly Tyr Glu Leu Leu Glu 50 55 60 ata att tcc gcg tta aaagaa aca tct gat acg aca gaa tta ttt tat 240 Ile Ile Ser Ala Leu Lys GluThr Ser Asp Thr Thr Glu Leu Phe Tyr 65 70 75 80 gag ccg ggt atc gtg cctttg ctg att gct tta tat aaa gaa gat agt 288 Glu Pro Gly Ile Val Pro LeuLeu Ile Ala Leu Tyr Lys Glu Asp Ser 85 90 95 tat ctg gta aat atg gtc aaacgg ata ttc aaa 321 Tyr Leu Val Asn Met Val Lys Arg Ile Phe Lys 100 10520 107 PRT Neisseria meningitidis 20 Met Phe Lys Asp Glu Leu Asn Glu PheIle Arg Leu Ile Ser Asp Pro 1 5 10 15 Glu Ser Glu Leu Asp Glu Trp TyrLeu Ser Asp Phe Lys Asp Glu His 20 25 30 Ile Trp Glu Met Gln Ser Tyr GluAla Phe Ser Cys Leu Arg Glu Ala 35 40 45 Val Pro Tyr Leu Phe Ala Tyr ProArg Tyr Gly Tyr Glu Leu Leu Glu 50 55 60 Ile Ile Ser Ala Leu Lys Glu ThrSer Asp Thr Thr Glu Leu Phe Tyr 65 70 75 80 Glu Pro Gly Ile Val Pro LeuLeu Ile Ala Leu Tyr Lys Glu Asp Ser 85 90 95 Tyr Leu Val Asn Met Val LysArg Ile Phe Lys 100 105 21 279 DNA Neisseria meningitidis CDS (1)..(279)NMA0038 21 atg gaa aac ggg caa cgg aat aac cgg ttt cct ttg gaa aaa cgaatt 48 Met Glu Asn Gly Gln Arg Asn Asn Arg Phe Pro Leu Glu Lys Arg Ile 15 10 15 ttt tat ctg gag cat tcg ggg cgg tat ctg atg att tgc gcc tta tcg96 Phe Tyr Leu Glu His Ser Gly Arg Tyr Leu Met Ile Cys Ala Leu Ser 20 2530 gat tat tcc caa aac aaa cat act gtc gtt atg gca aat ttc atc tat 144Asp Tyr Ser Gln Asn Lys His Thr Val Val Met Ala Asn Phe Ile Tyr 35 40 45ccg gat gaa aaa acg gat tgg cgg aat ttg gat gat tta ttc aat gag 192 ProAsp Glu Lys Thr Asp Trp Arg Asn Leu Asp Asp Leu Phe Asn Glu 50 55 60 ttggtt tta gag gaa ttg cag gct tca ttt atg gat tgg cat ccg act 240 Leu ValLeu Glu Glu Leu Gln Ala Ser Phe Met Asp Trp His Pro Thr 65 70 75 80 gttgaa gag gca atc agc cgt cat ttg gaa gac ttt tcg 279 Val Glu Glu Ala IleSer Arg His Leu Glu Asp Phe Ser 85 90 22 93 PRT Neisseria meningitidis22 Met Glu Asn Gly Gln Arg Asn Asn Arg Phe Pro Leu Glu Lys Arg Ile 1 510 15 Phe Tyr Leu Glu His Ser Gly Arg Tyr Leu Met Ile Cys Ala Leu Ser 2025 30 Asp Tyr Ser Gln Asn Lys His Thr Val Val Met Ala Asn Phe Ile Tyr 3540 45 Pro Asp Glu Lys Thr Asp Trp Arg Asn Leu Asp Asp Leu Phe Asn Glu 5055 60 Leu Val Leu Glu Glu Leu Gln Ala Ser Phe Met Asp Trp His Pro Thr 6570 75 80 Val Glu Glu Ala Ile Ser Arg His Leu Glu Asp Phe Ser 85 90 23471 DNA Neisseria meningitidis CDS (1)..(471) NMB0222 23 atg aac ctt gattta acc gcg caa aaa gtc cgt ctt tct tgg aag gat 48 Met Asn Leu Asp LeuThr Ala Gln Lys Val Arg Leu Ser Trp Lys Asp 1 5 10 15 att ctg tgg gggtat ggg aat aaa tac ttg ggt tgg gct gat gtg gca 96 Ile Leu Trp Gly TyrGly Asn Lys Tyr Leu Gly Trp Ala Asp Val Ala 20 25 30 gct tat gcc cga aaaatg acg ctt tca gat cat gat gaa cgt gtg ttc 144 Ala Tyr Ala Arg Lys MetThr Leu Ser Asp His Asp Glu Arg Val Phe 35 40 45 aaa cta tct tta atc aacaaa tcc aat att ctt gaa tta aag cct gtt 192 Lys Leu Ser Leu Ile Asn LysSer Asn Ile Leu Glu Leu Lys Pro Val 50 55 60 ctg gaa gat ttg gct tcg gaaatg agg gat tat tcc cct aaa aat tgg 240 Leu Glu Asp Leu Ala Ser Glu MetArg Asp Tyr Ser Pro Lys Asn Trp 65 70 75 80 ctg tac gtc ctc tta agc gatgta ttc cat aga aaa gaa gaa ttt gag 288 Leu Tyr Val Leu Leu Ser Asp ValPhe His Arg Lys Glu Glu Phe Glu 85 90 95 gat cct ttg ggg gaa gtt gaa aaaatt tat gca gat ttt gat tat ccg 336 Asp Pro Leu Gly Glu Val Glu Lys IleTyr Ala Asp Phe Asp Tyr Pro 100 105 110 gaa gaa ata gaa tca ttt gtc aggtat atg ccg ccc aaa gac ggt tat 384 Glu Glu Ile Glu Ser Phe Val Arg TyrMet Pro Pro Lys Asp Gly Tyr 115 120 125 att cct tct gcc cac acc tat gaagaa aat att gcc cgg tta tat tct 432 Ile Pro Ser Ala His Thr Tyr Glu GluAsn Ile Ala Arg Leu Tyr Ser 130 135 140 cac tgg gaa cac tat ttg aac aacggc gga ggg cag ggt 471 His Trp Glu His Tyr Leu Asn Asn Gly Gly Gly GlnGly 145 150 155 24 157 PRT Neisseria meningitidis 24 Met Asn Leu Asp LeuThr Ala Gln Lys Val Arg Leu Ser Trp Lys Asp 1 5 10 15 Ile Leu Trp GlyTyr Gly Asn Lys Tyr Leu Gly Trp Ala Asp Val Ala 20 25 30 Ala Tyr Ala ArgLys Met Thr Leu Ser Asp His Asp Glu Arg Val Phe 35 40 45 Lys Leu Ser LeuIle Asn Lys Ser Asn Ile Leu Glu Leu Lys Pro Val 50 55 60 Leu Glu Asp LeuAla Ser Glu Met Arg Asp Tyr Ser Pro Lys Asn Trp 65 70 75 80 Leu Tyr ValLeu Leu Ser Asp Val Phe His Arg Lys Glu Glu Phe Glu 85 90 95 Asp Pro LeuGly Glu Val Glu Lys Ile Tyr Ala Asp Phe Asp Tyr Pro 100 105 110 Glu GluIle Glu Ser Phe Val Arg Tyr Met Pro Pro Lys Asp Gly Tyr 115 120 125 IlePro Ser Ala His Thr Tyr Glu Glu Asn Ile Ala Arg Leu Tyr Ser 130 135 140His Trp Glu His Tyr Leu Asn Asn Gly Gly Gly Gln Gly 145 150 155 25 333DNA Neisseria meningitidis CDS (1)..(333) NMA0036 25 atg acg gtt gat aaggaa caa gta gaa aag atg att tat tct gaa aat 48 Met Thr Val Asp Lys GluGln Val Glu Lys Met Ile Tyr Ser Glu Asn 1 5 10 15 aaa cag agt gtt attgac ggt atg ctc ggt atg acc ttc agc tcg gag 96 Lys Gln Ser Val Ile AspGly Met Leu Gly Met Thr Phe Ser Ser Glu 20 25 30 gag gat gaa atc ccc tggatt tcc gag aag ttg acg gag ttg tcg aaa 144 Glu Asp Glu Ile Pro Trp IleSer Glu Lys Leu Thr Glu Leu Ser Lys 35 40 45 cat aaa gac ttg gat att gccaga ttg tca tta acc tgt ttc gga cat 192 His Lys Asp Leu Asp Ile Ala ArgLeu Ser Leu Thr Cys Phe Gly His 50 55 60 ctc gcc agg atg cat gaa aat atcggc gat tgc gat aaa gtg att gct 240 Leu Ala Arg Met His Glu Asn Ile GlyAsp Cys Asp Lys Val Ile Ala 65 70 75 80 tta ttg ctc tca aaa caa ggt gatccg gat ttt caa ggt ttt gcc gaa 288 Leu Leu Leu Ser Lys Gln Gly Asp ProAsp Phe Gln Gly Phe Ala Glu 85 90 95 gat gcg tta gac gag att tct tta tttata ttt aaa aag cgt ccg 333 Asp Ala Leu Asp Glu Ile Ser Leu Phe Ile PheLys Lys Arg Pro 100 105 110 26 111 PRT Neisseria meningitidis 26 Met ThrVal Asp Lys Glu Gln Val Glu Lys Met Ile Tyr Ser Glu Asn 1 5 10 15 LysGln Ser Val Ile Asp Gly Met Leu Gly Met Thr Phe Ser Ser Glu 20 25 30 GluAsp Glu Ile Pro Trp Ile Ser Glu Lys Leu Thr Glu Leu Ser Lys 35 40 45 HisLys Asp Leu Asp Ile Ala Arg Leu Ser Leu Thr Cys Phe Gly His 50 55 60 LeuAla Arg Met His Glu Asn Ile Gly Asp Cys Asp Lys Val Ile Ala 65 70 75 80Leu Leu Leu Ser Lys Gln Gly Asp Pro Asp Phe Gln Gly Phe Ala Glu 85 90 95Asp Ala Leu Asp Glu Ile Ser Leu Phe Ile Phe Lys Lys Arg Pro 100 105 11027 1338 DNA Neisseria meningitidis CDS (1)..(1338) NMB1645 27 atg ttgaat cca tcc cga aaa ctg gtt gag ctg gtc cgt att ttg gac 48 Met Leu AsnPro Ser Arg Lys Leu Val Glu Leu Val Arg Ile Leu Asp 1 5 10 15 gaa ggcggt ttt att ttc agc ggc gat ccc gta cag gcg acg gag gct 96 Glu Gly GlyPhe Ile Phe Ser Gly Asp Pro Val Gln Ala Thr Glu Ala 20 25 30 ttg cgc cgcgtg gac ggc agt acg gag gaa aaa atc atc cgt cgg gcg 144 Leu Arg Arg ValAsp Gly Ser Thr Glu Glu Lys Ile Ile Arg Arg Ala 35 40 45 gag atg att gacagg aac cgt atg ctg cgg gag acg ttg gaa cgt gtg 192 Glu Met Ile Asp ArgAsn Arg Met Leu Arg Glu Thr Leu Glu Arg Val 50 55 60 cgt gcg ggg tcg ttctgg ttg tgg gtg gtg gcg gcg acg ttt gca ttt 240 Arg Ala Gly Ser Phe TrpLeu Trp Val Val Ala Ala Thr Phe Ala Phe 65 70 75 80 ttt acc ggt ttt tcagtc act tat ctt cta atg gac aat cag ggt ctg 288 Phe Thr Gly Phe Ser ValThr Tyr Leu Leu Met Asp Asn Gln Gly Leu 85 90 95 aat ttc ttt ttg gtt ttggcg ggc gtg ttg ggc atg aat acg ctg atg 336 Asn Phe Phe Leu Val Leu AlaGly Val Leu Gly Met Asn Thr Leu Met 100 105 110 ctg gca gta tgg ttg gcaatg ttg ttc ctg cgt gtg aaa gtg ggg cgt 384 Leu Ala Val Trp Leu Ala MetLeu Phe Leu Arg Val Lys Val Gly Arg 115 120 125 ttt ttc agc agt ccg gcgacg tgg ttt cgg ggc aaa gac cct gta aat 432 Phe Phe Ser Ser Pro Ala ThrTrp Phe Arg Gly Lys Asp Pro Val Asn 130 135 140 cag gcg gtg ttg cgg ctgtat gcg gac gag tgg cgg caa cct tcg gta 480 Gln Ala Val Leu Arg Leu TyrAla Asp Glu Trp Arg Gln Pro Ser Val 145 150 155 160 cgt tgg aaa ata ggcgca acg tcg cac agc ctg tgg ctc tgc acg ctg 528 Arg Trp Lys Ile Gly AlaThr Ser His Ser Leu Trp Leu Cys Thr Leu 165 170 175 ctc gga atg ctg gtgtcg gta ttg ttg ctg ctt ttg gtg cgg caa tat 576 Leu Gly Met Leu Val SerVal Leu Leu Leu Leu Leu Val Arg Gln Tyr 180 185 190 acg ttc aac tgg gaaagc acg ctg ttg agc aat gcc gct tcg gta cgc 624 Thr Phe Asn Trp Glu SerThr Leu Leu Ser Asn Ala Ala Ser Val Arg 195 200 205 gcg gtg gaa atg ttggca tgg ctg ccg tcg aaa ctc ggt ttc cct gtc 672 Ala Val Glu Met Leu AlaTrp Leu Pro Ser Lys Leu Gly Phe Pro Val 210 215 220 ccc gat gcg cgg gcggtc atc gaa ggc cgt ctg aac ggc aat att gcc 720 Pro Asp Ala Arg Ala ValIle Glu Gly Arg Leu Asn Gly Asn Ile Ala 225 230 235 240 gat gcg cgg gcttgg tcg ggg ctg ctg gtc ggc agt atc gcc tgc tac 768 Asp Ala Arg Ala TrpSer Gly Leu Leu Val Gly Ser Ile Ala Cys Tyr 245 250 255 ggc atc ctg ccgcgc ctg ctg gct tgg gta gtg tgt aaa atc ctt ttg 816 Gly Ile Leu Pro ArgLeu Leu Ala Trp Val Val Cys Lys Ile Leu Leu 260 265 270 aaa aca agc gaaaac gga ttg gat ttg gaa aag ccc tat tat cag gcg 864 Lys Thr Ser Glu AsnGly Leu Asp Leu Glu Lys Pro Tyr Tyr Gln Ala 275 280 285 gtc atc cgc cgctgg cag aac aaa atc acc gat gcg gat acg cgt cgg 912 Val Ile Arg Arg TrpGln Asn Lys Ile Thr Asp Ala Asp Thr Arg Arg 290 295 300 gaa acc gtg tccgcc gtt tca ccg aaa atc atc ttg aac gat gcg ccg 960 Glu Thr Val Ser AlaVal Ser Pro Lys Ile Ile Leu Asn Asp Ala Pro 305 310 315 320 aaa tgg gcggtc atg ctg gag acc gag tgg cag gac ggc gaa tgg ttc 1008 Lys Trp Ala ValMet Leu Glu Thr Glu Trp Gln Asp Gly Glu Trp Phe 325 330 335 gag ggc aggctg gcg cag gaa tgg ctg gat aag ggc gtt gcc acc aat 1056 Glu Gly Arg LeuAla Gln Glu Trp Leu Asp Lys Gly Val Ala Thr Asn 340 345 350 cgg gaa caggtt gcc gcg ctg gag aca gag ctg aag cag aaa ccg gcg 1104 Arg Glu Gln ValAla Ala Leu Glu Thr Glu Leu Lys Gln Lys Pro Ala 355 360 365 caa ctg cttatc ggc gtg cgc gcc caa act gtg ccg gac cgc ggc gtg 1152 Gln Leu Leu IleGly Val Arg Ala Gln Thr Val Pro Asp Arg Gly Val 370 375 380 ttg cgg cagatt gtc cga ctc tcg gaa gcg gcg cag ggc ggc gcg gtg 1200 Leu Arg Gln IleVal Arg Leu Ser Glu Ala Ala Gln Gly Gly Ala Val 385 390 395 400 gtg cagctt ttg gcg gaa cag ggg ctt tca gac gac ctt tcg gaa aag 1248 Val Gln LeuLeu Ala Glu Gln Gly Leu Ser Asp Asp Leu Ser Glu Lys 405 410 415 ctg gaacat tgg cgt aac gcg ctg gcc gaa tgc ggc gcg gcg tgg ctt 1296 Leu Glu HisTrp Arg Asn Ala Leu Ala Glu Cys Gly Ala Ala Trp Leu 420 425 430 gag cctgac agg gcg gcg cag gaa ggg cgt ttg aaa gac caa 1338 Glu Pro Asp Arg AlaAla Gln Glu Gly Arg Leu Lys Asp Gln 435 440 445 28 446 PRT Neisseriameningitidis 28 Met Leu Asn Pro Ser Arg Lys Leu Val Glu Leu Val Arg IleLeu Asp 1 5 10 15 Glu Gly Gly Phe Ile Phe Ser Gly Asp Pro Val Gln AlaThr Glu Ala 20 25 30 Leu Arg Arg Val Asp Gly Ser Thr Glu Glu Lys Ile IleArg Arg Ala 35 40 45 Glu Met Ile Asp Arg Asn Arg Met Leu Arg Glu Thr LeuGlu Arg Val 50 55 60 Arg Ala Gly Ser Phe Trp Leu Trp Val Val Ala Ala ThrPhe Ala Phe 65 70 75 80 Phe Thr Gly Phe Ser Val Thr Tyr Leu Leu Met AspAsn Gln Gly Leu 85 90 95 Asn Phe Phe Leu Val Leu Ala Gly Val Leu Gly MetAsn Thr Leu Met 100 105 110 Leu Ala Val Trp Leu Ala Met Leu Phe Leu ArgVal Lys Val Gly Arg 115 120 125 Phe Phe Ser Ser Pro Ala Thr Trp Phe ArgGly Lys Asp Pro Val Asn 130 135 140 Gln Ala Val Leu Arg Leu Tyr Ala AspGlu Trp Arg Gln Pro Ser Val 145 150 155 160 Arg Trp Lys Ile Gly Ala ThrSer His Ser Leu Trp Leu Cys Thr Leu 165 170 175 Leu Gly Met Leu Val SerVal Leu Leu Leu Leu Leu Val Arg Gln Tyr 180 185 190 Thr Phe Asn Trp GluSer Thr Leu Leu Ser Asn Ala Ala Ser Val Arg 195 200 205 Ala Val Glu MetLeu Ala Trp Leu Pro Ser Lys Leu Gly Phe Pro Val 210 215 220 Pro Asp AlaArg Ala Val Ile Glu Gly Arg Leu Asn Gly Asn Ile Ala 225 230 235 240 AspAla Arg Ala Trp Ser Gly Leu Leu Val Gly Ser Ile Ala Cys Tyr 245 250 255Gly Ile Leu Pro Arg Leu Leu Ala Trp Val Val Cys Lys Ile Leu Leu 260 265270 Lys Thr Ser Glu Asn Gly Leu Asp Leu Glu Lys Pro Tyr Tyr Gln Ala 275280 285 Val Ile Arg Arg Trp Gln Asn Lys Ile Thr Asp Ala Asp Thr Arg Arg290 295 300 Glu Thr Val Ser Ala Val Ser Pro Lys Ile Ile Leu Asn Asp AlaPro 305 310 315 320 Lys Trp Ala Val Met Leu Glu Thr Glu Trp Gln Asp GlyGlu Trp Phe 325 330 335 Glu Gly Arg Leu Ala Gln Glu Trp Leu Asp Lys GlyVal Ala Thr Asn 340 345 350 Arg Glu Gln Val Ala Ala Leu Glu Thr Glu LeuLys Gln Lys Pro Ala 355 360 365 Gln Leu Leu Ile Gly Val Arg Ala Gln ThrVal Pro Asp Arg Gly Val 370 375 380 Leu Arg Gln Ile Val Arg Leu Ser GluAla Ala Gln Gly Gly Ala Val 385 390 395 400 Val Gln Leu Leu Ala Glu GlnGly Leu Ser Asp Asp Leu Ser Glu Lys 405 410 415 Leu Glu His Trp Arg AsnAla Leu Ala Glu Cys Gly Ala Ala Trp Leu 420 425 430 Glu Pro Asp Arg AlaAla Gln Glu Gly Arg Leu Lys Asp Gln 435 440 445 29 1392 DNA Neisseriameningitidis CDS (1)..(1392) NMA1898 29 atg aac aaa caa ccc ctt tcc ctcgcc gtc gtc ggg cat acc aat acc 48 Met Asn Lys Gln Pro Leu Ser Leu AlaVal Val Gly His Thr Asn Thr 1 5 10 15 ggc aaa acc tcg ctc ctg cgc acccta ttg cgc gac agc ggc ttc ggc 96 Gly Lys Thr Ser Leu Leu Arg Thr LeuLeu Arg Asp Ser Gly Phe Gly 20 25 30 gaa gtc aaa aat gcc ccg tcc act acgcgc cat gtc gaa gaa gcc gcc 144 Glu Val Lys Asn Ala Pro Ser Thr Thr ArgHis Val Glu Glu Ala Ala 35 40 45 atc agc gac ggc gca gac acg ctg gtt ttcctg tat gac acg ccc gga 192 Ile Ser Asp Gly Ala Asp Thr Leu Val Phe LeuTyr Asp Thr Pro Gly 50 55 60 ctc gaa gac gcg ggc ggt gtt ttg gaa tgg ctggaa acc cat acg gat 240 Leu Glu Asp Ala Gly Gly Val Leu Glu Trp Leu GluThr His Thr Asp 65 70 75 80 acg cgt tca gac ggc atc gaa cgg ctg caa cagttt ctc ggc agc cac 288 Thr Arg Ser Asp Gly Ile Glu Arg Leu Gln Gln PheLeu Gly Ser His 85 90 95 ggc gcg cac cat gat ttc aat cag gaa gcc aaa gtctta cgg caa gtc 336 Gly Ala His His Asp Phe Asn Gln Glu Ala Lys Val LeuArg Gln Val 100 105 110 ttg caa agc gat atg gca atg tac gtc atc gac gcgcgc gaa ccc gtc 384 Leu Gln Ser Asp Met Ala Met Tyr Val Ile Asp Ala ArgGlu Pro Val 115 120 125 ctc gac aaa tac agg gac gag ttg acc atc ctt tcatgg tgt gcc aaa 432 Leu Asp Lys Tyr Arg Asp Glu Leu Thr Ile Leu Ser TrpCys Ala Lys 130 135 140 ccg gtt atg ccc gtg ttc aac ttt acc ggc gga cagcct ccc gaa tcg 480 Pro Val Met Pro Val Phe Asn Phe Thr Gly Gly Gln ProPro Glu Ser 145 150 155 160 tgg aca acc atg ctg gcg agg aga aac ctg cacgtt ttc gca ggg ttc 528 Trp Thr Thr Met Leu Ala Arg Arg Asn Leu His ValPhe Ala Gly Phe 165 170 175 gac acc gtc gcc ttt gat ttt gaa ggc gaa ctacgc ctg tgg gaa aac 576 Asp Thr Val Ala Phe Asp Phe Glu Gly Glu Leu ArgLeu Trp Glu Asn 180 185 190 ctc gcc acc atg ttg ccc gaa cgc agc aca cttgac cgc ctg aca gcc 624 Leu Ala Thr Met Leu Pro Glu Arg Ser Thr Leu AspArg Leu Thr Ala 195 200 205 atg cgc cgg cgc gaa tgg cag cgg ctg gac ggcgaa gcg cgc cgc gaa 672 Met Arg Arg Arg Glu Trp Gln Arg Leu Asp Gly GluAla Arg Arg Glu 210 215 220 atc gcc gac ttt tta att gat gcc gcc gcc ttcagg cag gaa gtg gac 720 Ile Ala Asp Phe Leu Ile Asp Ala Ala Ala Phe ArgGln Glu Val Asp 225 230 235 240 gaa aac gag gat acc gcc acc gtg ccg caaacc atg cag gcg gaa ata 768 Glu Asn Glu Asp Thr Ala Thr Val Pro Gln ThrMet Gln Ala Glu Ile 245 250 255 cgc caa ctc gaa cgg cag atg cag cag cggctg ttt gcc ctt tac cgt 816 Arg Gln Leu Glu Arg Gln Met Gln Gln Arg LeuPhe Ala Leu Tyr Arg 260 265 270 ttc tac cac agc gaa atc gac ggc ggc gactgg atg ccg caa gcc ttc 864 Phe Tyr His Ser Glu Ile Asp Gly Gly Asp TrpMet Pro Gln Ala Phe 275 280 285 cgc caa gac ccg ttc gac agc gaa ttg ctcaaa caa tac ggc atc cgc 912 Arg Gln Asp Pro Phe Asp Ser Glu Leu Leu LysGln Tyr Gly Ile Arg 290 295 300 acc ggc acg ggc gcg gca acc ggc gcg ctcatc ggc ttg ggg ctg gac 960 Thr Gly Thr Gly Ala Ala Thr Gly Ala Leu IleGly Leu Gly Leu Asp 305 310 315 320 atc gcc aca ctc ggc ggc tcg ctc ggattg ggt acg gca atc ggc ggc 1008 Ile Ala Thr Leu Gly Gly Ser Leu Gly LeuGly Thr Ala Ile Gly Gly 325 330 335 ttt ttg ggc ggc atc ctg ccc aat acccgc acc att tct gac aaa ctc 1056 Phe Leu Gly Gly Ile Leu Pro Asn Thr ArgThr Ile Ser Asp Lys Leu 340 345 350 gcc ggc cgt caa acc ctg cac acc gacccc gaa acc ctg acc ctg ctc 1104 Ala Gly Arg Gln Thr Leu His Thr Asp ProGlu Thr Leu Thr Leu Leu 355 360 365 gcc gcc cgc gcc ctc gat ctg ctc cacgtc ctg caa acg cgc gga cac 1152 Ala Ala Arg Ala Leu Asp Leu Leu His ValLeu Gln Thr Arg Gly His 370 375 380 gcg gca cag tcg gat atc gag ctg cacagc cgc aaa gtc ccg tgg gat 1200 Ala Ala Gln Ser Asp Ile Glu Leu His SerArg Lys Val Pro Trp Asp 385 390 395 400 gcc gcc aga ctc ccg ccc gaa ctcaac aaa gcc cgc agt cat tgg aaa 1248 Ala Ala Arg Leu Pro Pro Glu Leu AsnLys Ala Arg Ser His Trp Lys 405 410 415 tgg tcg tcg ctc aat acg cac cgcccg gaa acc agc cgc gcc gaa cgc 1296 Trp Ser Ser Leu Asn Thr His Arg ProGlu Thr Ser Arg Ala Glu Arg 420 425 430 gcc gga tat gtg gag aaa ctt caaatc cgc ctg tcg gga aaa tat agt 1344 Ala Gly Tyr Val Glu Lys Leu Gln IleArg Leu Ser Gly Lys Tyr Ser 435 440 445 gaa tta aat tta aat cag gac aaggcg gcg agc cgc aga cag tac aaa 1392 Glu Leu Asn Leu Asn Gln Asp Lys AlaAla Ser Arg Arg Gln Tyr Lys 450 455 460 30 464 PRT Neisseriameningitidis 30 Met Asn Lys Gln Pro Leu Ser Leu Ala Val Val Gly His ThrAsn Thr 1 5 10 15 Gly Lys Thr Ser Leu Leu Arg Thr Leu Leu Arg Asp SerGly Phe Gly 20 25 30 Glu Val Lys Asn Ala Pro Ser Thr Thr Arg His Val GluGlu Ala Ala 35 40 45 Ile Ser Asp Gly Ala Asp Thr Leu Val Phe Leu Tyr AspThr Pro Gly 50 55 60 Leu Glu Asp Ala Gly Gly Val Leu Glu Trp Leu Glu ThrHis Thr Asp 65 70 75 80 Thr Arg Ser Asp Gly Ile Glu Arg Leu Gln Gln PheLeu Gly Ser His 85 90 95 Gly Ala His His Asp Phe Asn Gln Glu Ala Lys ValLeu Arg Gln Val 100 105 110 Leu Gln Ser Asp Met Ala Met Tyr Val Ile AspAla Arg Glu Pro Val 115 120 125 Leu Asp Lys Tyr Arg Asp Glu Leu Thr IleLeu Ser Trp Cys Ala Lys 130 135 140 Pro Val Met Pro Val Phe Asn Phe ThrGly Gly Gln Pro Pro Glu Ser 145 150 155 160 Trp Thr Thr Met Leu Ala ArgArg Asn Leu His Val Phe Ala Gly Phe 165 170 175 Asp Thr Val Ala Phe AspPhe Glu Gly Glu Leu Arg Leu Trp Glu Asn 180 185 190 Leu Ala Thr Met LeuPro Glu Arg Ser Thr Leu Asp Arg Leu Thr Ala 195 200 205 Met Arg Arg ArgGlu Trp Gln Arg Leu Asp Gly Glu Ala Arg Arg Glu 210 215 220 Ile Ala AspPhe Leu Ile Asp Ala Ala Ala Phe Arg Gln Glu Val Asp 225 230 235 240 GluAsn Glu Asp Thr Ala Thr Val Pro Gln Thr Met Gln Ala Glu Ile 245 250 255Arg Gln Leu Glu Arg Gln Met Gln Gln Arg Leu Phe Ala Leu Tyr Arg 260 265270 Phe Tyr His Ser Glu Ile Asp Gly Gly Asp Trp Met Pro Gln Ala Phe 275280 285 Arg Gln Asp Pro Phe Asp Ser Glu Leu Leu Lys Gln Tyr Gly Ile Arg290 295 300 Thr Gly Thr Gly Ala Ala Thr Gly Ala Leu Ile Gly Leu Gly LeuAsp 305 310 315 320 Ile Ala Thr Leu Gly Gly Ser Leu Gly Leu Gly Thr AlaIle Gly Gly 325 330 335 Phe Leu Gly Gly Ile Leu Pro Asn Thr Arg Thr IleSer Asp Lys Leu 340 345 350 Ala Gly Arg Gln Thr Leu His Thr Asp Pro GluThr Leu Thr Leu Leu 355 360 365 Ala Ala Arg Ala Leu Asp Leu Leu His ValLeu Gln Thr Arg Gly His 370 375 380 Ala Ala Gln Ser Asp Ile Glu Leu HisSer Arg Lys Val Pro Trp Asp 385 390 395 400 Ala Ala Arg Leu Pro Pro GluLeu Asn Lys Ala Arg Ser His Trp Lys 405 410 415 Trp Ser Ser Leu Asn ThrHis Arg Pro Glu Thr Ser Arg Ala Glu Arg 420 425 430 Ala Gly Tyr Val GluLys Leu Gln Ile Arg Leu Ser Gly Lys Tyr Ser 435 440 445 Glu Leu Asn LeuAsn Gln Asp Lys Ala Ala Ser Arg Arg Gln Tyr Lys 450 455 460 31 2622 DNANeisseria meningitidis CDS (1)..(2622) NMB1595 31 atg aaa acc tcc gaactg cgc caa aaa ttc cta aaa ttt ttt gaa acc 48 Met Lys Thr Ser Glu LeuArg Gln Lys Phe Leu Lys Phe Phe Glu Thr 1 5 10 15 aaa ggc cac acc gtcgtc cgc tct tcc agc ctc gtg ccg cac gac gac 96 Lys Gly His Thr Val ValArg Ser Ser Ser Leu Val Pro His Asp Asp 20 25 30 ccg acc ctg ctg ttt accaac gcg ggc atg aac cag ttt aaa gac gta 144 Pro Thr Leu Leu Phe Thr AsnAla Gly Met Asn Gln Phe Lys Asp Val 35 40 45 ttc tta ggt ttc gac aaa cgcccg tac agc cgc gcc acc acc gcg caa 192 Phe Leu Gly Phe Asp Lys Arg ProTyr Ser Arg Ala Thr Thr Ala Gln 50 55 60 aaa tgc gta cgc gca ggc ggc aaacac aac gac ttg gaa aac gtc ggc 240 Lys Cys Val Arg Ala Gly Gly Lys HisAsn Asp Leu Glu Asn Val Gly 65 70 75 80 tac acc gcc cgc cac cac acc ttcttt gaa atg atg ggc aac ttc tcc 288 Tyr Thr Ala Arg His His Thr Phe PheGlu Met Met Gly Asn Phe Ser 85 90 95 ttc ggc gac tac ttc aaa cgc gac gccatc cac ttc gct tgg gaa ttt 336 Phe Gly Asp Tyr Phe Lys Arg Asp Ala IleHis Phe Ala Trp Glu Phe 100 105 110 ctg act tcc ccc gaa tgg ctc aac atccct aaa gac aaa ctg ttg gcg 384 Leu Thr Ser Pro Glu Trp Leu Asn Ile ProLys Asp Lys Leu Leu Ala 115 120 125 acc gtt tac gcg gaa gac gac gaa gcctac aac atc tgg ttg aac gaa 432 Thr Val Tyr Ala Glu Asp Asp Glu Ala TyrAsn Ile Trp Leu Asn Glu 130 135 140 atc ggt atg ccg tcc gag cgc atc gtccgc atc ggc gac aac aaa ggc 480 Ile Gly Met Pro Ser Glu Arg Ile Val ArgIle Gly Asp Asn Lys Gly 145 150 155 160 gcg aaa tac gca tcc gac aac ttctgg caa atg ggc gac acc ggc cct 528 Ala Lys Tyr Ala Ser Asp Asn Phe TrpGln Met Gly Asp Thr Gly Pro 165 170 175 tgc ggc ccc tgc tcc gaa att ttctac gac cac ggc gaa gaa atc tgg 576 Cys Gly Pro Cys Ser Glu Ile Phe TyrAsp His Gly Glu Glu Ile Trp 180 185 190 ggc ggc att ccc ggc agt ccc gaagaa gac ggc gac cgc tgg atc gaa 624 Gly Gly Ile Pro Gly Ser Pro Glu GluAsp Gly Asp Arg Trp Ile Glu 195 200 205 att tgg aac tgc gta ttt atg cagttc aac cgc gac gaa caa ggc aat 672 Ile Trp Asn Cys Val Phe Met Gln PheAsn Arg Asp Glu Gln Gly Asn 210 215 220 atg aac ccg ctt ccc aaa cct tccgtc gat acc ggt atg ggc ttg gaa 720 Met Asn Pro Leu Pro Lys Pro Ser ValAsp Thr Gly Met Gly Leu Glu 225 230 235 240 cgc ata gcc gcc gtc atg cagcat gtt cac agc aac tac gaa atc gac 768 Arg Ile Ala Ala Val Met Gln HisVal His Ser Asn Tyr Glu Ile Asp 245 250 255 ttg ttc caa gac ctg ctc aaagcc gtt gcc cgc gaa acc ggc gcg ccg 816 Leu Phe Gln Asp Leu Leu Lys AlaVal Ala Arg Glu Thr Gly Ala Pro 260 265 270 ttc aga atg gaa gaa ccc agcctg aaa gtc atc gcc gac cac atc cgc 864 Phe Arg Met Glu Glu Pro Ser LeuLys Val Ile Ala Asp His Ile Arg 275 280 285 tcc tgc tcg ttc ctg att gcagac ggc gtc ttg cct tcc aac gaa ggc 912 Ser Cys Ser Phe Leu Ile Ala AspGly Val Leu Pro Ser Asn Glu Gly 290 295 300 cgc ggc tac gta ttg cgc cgcatt atc cgc cgc gcc gtg cgc cac ggt 960 Arg Gly Tyr Val Leu Arg Arg IleIle Arg Arg Ala Val Arg His Gly 305 310 315 320 tac aaa ctg ggt caa agcaaa ccg ttc ttc cac aaa ctc gtt gcc gat 1008 Tyr Lys Leu Gly Gln Ser LysPro Phe Phe His Lys Leu Val Ala Asp 325 330 335 ttg gtc aaa gag atg ggcggt gcc tac cct gaa ttg aaa gaa aaa caa 1056 Leu Val Lys Glu Met Gly GlyAla Tyr Pro Glu Leu Lys Glu Lys Gln 340 345 350 gcc caa atc gaa gaa gcattg aaa aac gaa gaa agc cgt ttt gcc caa 1104 Ala Gln Ile Glu Glu Ala LeuLys Asn Glu Glu Ser Arg Phe Ala Gln 355 360 365 acg ctg gaa acc ggt atggct ttg ttg gaa aac gcg ctg gtc aaa ggc 1152 Thr Leu Glu Thr Gly Met AlaLeu Leu Glu Asn Ala Leu Val Lys Gly 370 375 380 ggc aaa aca ctc ggc ggcgaa atc atc ttc aaa ctc tac gat acc tac 1200 Gly Lys Thr Leu Gly Gly GluIle Ile Phe Lys Leu Tyr Asp Thr Tyr 385 390 395 400 ggt ttc cca tac gacttg act gcc gac atc tgc cgc gaa cgc aat atc 1248 Gly Phe Pro Tyr Asp LeuThr Ala Asp Ile Cys Arg Glu Arg Asn Ile 405 410 415 gaa ccg gac gaa gcaggc ttc gag cgc gaa atg gaa gcc caa cgc gca 1296 Glu Pro Asp Glu Ala GlyPhe Glu Arg Glu Met Glu Ala Gln Arg Ala 420 425 430 cgc gca cgc gcc gcccaa agc ttc aaa gcc aac gcc caa ctg cct tat 1344 Arg Ala Arg Ala Ala GlnSer Phe Lys Ala Asn Ala Gln Leu Pro Tyr 435 440 445 gac ggt caa gac accgag ttt aaa ggt tat agc gaa cgc caa acc gaa 1392 Asp Gly Gln Asp Thr GluPhe Lys Gly Tyr Ser Glu Arg Gln Thr Glu 450 455 460 tcc aaa gtc ctc gccctc tac aaa gac ggc gag caa gtc aac gaa ttg 1440 Ser Lys Val Leu Ala LeuTyr Lys Asp Gly Glu Gln Val Asn Glu Leu 465 470 475 480 aac gaa ggc gacagc ggc gca gtc gtc atc gac ttt acc ccg ttc tat 1488 Asn Glu Gly Asp SerGly Ala Val Val Ile Asp Phe Thr Pro Phe Tyr 485 490 495 gca gaa tcc ggcggc caa gtc ggc gat gtc ggc tat atc ttc tca ggc 1536 Ala Glu Ser Gly GlyGln Val Gly Asp Val Gly Tyr Ile Phe Ser Gly 500 505 510 gaa aac cgc tttgaa gta cgc gat acc caa aaa atc aaa gcg gcc gta 1584 Glu Asn Arg Phe GluVal Arg Asp Thr Gln Lys Ile Lys Ala Ala Val 515 520 525 ttc ggt caa ttcggc gta caa act tca ggc cgt ctg aaa gtc ggc gac 1632 Phe Gly Gln Phe GlyVal Gln Thr Ser Gly Arg Leu Lys Val Gly Asp 530 535 540 agc gtt acc gccaaa gtg gac gac gaa atc cgc aat gcc aat atg cgc 1680 Ser Val Thr Ala LysVal Asp Asp Glu Ile Arg Asn Ala Asn Met Arg 545 550 555 560 aac cac agcgca acc cac ttg atg cac aaa gcc ctg cgc gat gta ttg 1728 Asn His Ser AlaThr His Leu Met His Lys Ala Leu Arg Asp Val Leu 565 570 575 ggc aga cacgtc gaa caa aaa ggc tct ttg gtt acc gcc gaa tcc acc 1776 Gly Arg His ValGlu Gln Lys Gly Ser Leu Val Thr Ala Glu Ser Thr 580 585 590 cgt ttc gacatt tcc cat ccc caa gcg gta act gcc gaa gaa att gcc 1824 Arg Phe Asp IleSer His Pro Gln Ala Val Thr Ala Glu Glu Ile Ala 595 600 605 gaa gta gaacgc cgc gtc aac gaa gcc att ttg gcg aac gtt gcc gtc 1872 Glu Val Glu ArgArg Val Asn Glu Ala Ile Leu Ala Asn Val Ala Val 610 615 620 aat gca gccatt atg agc atg gaa gac gcg caa aaa acc ggc gcg atg 1920 Asn Ala Ala IleMet Ser Met Glu Asp Ala Gln Lys Thr Gly Ala Met 625 630 635 640 atg ctcttc ggc gaa aaa tac ggc gaa gaa gtg cgc gta ctg caa atg 1968 Met Leu PheGly Glu Lys Tyr Gly Glu Glu Val Arg Val Leu Gln Met 645 650 655 ggc ggtttc tct acc gaa ttg tgc ggc ggc aca cac gtt tca cgc acc 2016 Gly Gly PheSer Thr Glu Leu Cys Gly Gly Thr His Val Ser Arg Thr 660 665 670 ggc gacatc ggc ctc ttc aaa atc atc agc gaa ggc ggt att gcc gca 2064 Gly Asp IleGly Leu Phe Lys Ile Ile Ser Glu Gly Gly Ile Ala Ala 675 680 685 ggc gtgcgc cgt atc gaa gcc atc acc ggc ctg aac gca ctc aaa tgg 2112 Gly Val ArgArg Ile Glu Ala Ile Thr Gly Leu Asn Ala Leu Lys Trp 690 695 700 gcg caagag caa gag cgt ttg gtg aaa gac att att gcc gaa acc aaa 2160 Ala Gln GluGln Glu Arg Leu Val Lys Asp Ile Ile Ala Glu Thr Lys 705 710 715 720 gcccaa acc gaa aaa gac gta ctg gca aaa atc caa gca ggc gcg gca 2208 Ala GlnThr Glu Lys Asp Val Leu Ala Lys Ile Gln Ala Gly Ala Ala 725 730 735 cacgcc aaa gca ttg gaa aaa gaa ttg gca cgc gcc aaa gcc gaa ctc 2256 His AlaLys Ala Leu Glu Lys Glu Leu Ala Arg Ala Lys Ala Glu Leu 740 745 750 gccgtc cac gca ggc gcc aaa ctc ttg gac gat gca aaa gac ttg ggc 2304 Ala ValHis Ala Gly Ala Lys Leu Leu Asp Asp Ala Lys Asp Leu Gly 755 760 765 gcagcc aaa ctc gtt gcc gcc caa atc gaa gcc gac gca gcc gcc ctg 2352 Ala AlaLys Leu Val Ala Ala Gln Ile Glu Ala Asp Ala Ala Ala Leu 770 775 780 cgcgaa atc gtt acc gat tta acc ggt aaa tcc gac aac gcc gtg att 2400 Arg GluIle Val Thr Asp Leu Thr Gly Lys Ser Asp Asn Ala Val Ile 785 790 795 800ctt tta gcg gca gta aac gac ggc aaa gtc tcc ctg tgc gcc ggc gta 2448 LeuLeu Ala Ala Val Asn Asp Gly Lys Val Ser Leu Cys Ala Gly Val 805 810 815tcc aaa ccg ttg acc ggc aaa gtg aaa gca ggc gat ctg gtt aaa ttt 2496 SerLys Pro Leu Thr Gly Lys Val Lys Ala Gly Asp Leu Val Lys Phe 820 825 830gca gcc gaa caa gtc ggc ggc aaa ggc ggc ggc aga cca gat ttg gcg 2544 AlaAla Glu Gln Val Gly Gly Lys Gly Gly Gly Arg Pro Asp Leu Ala 835 840 845caa gcc ggc ggc acg gat gcc gac aaa ttg ccc gcc gtg ttg gat agc 2592 GlnAla Gly Gly Thr Asp Ala Asp Lys Leu Pro Ala Val Leu Asp Ser 850 855 860gtg aaa gac tgg gtc ggc gcg aag ctg gtt 2622 Val Lys Asp Trp Val Gly AlaLys Leu Val 865 870 32 874 PRT Neisseria meningitidis 32 Met Lys Thr SerGlu Leu Arg Gln Lys Phe Leu Lys Phe Phe Glu Thr 1 5 10 15 Lys Gly HisThr Val Val Arg Ser Ser Ser Leu Val Pro His Asp Asp 20 25 30 Pro Thr LeuLeu Phe Thr Asn Ala Gly Met Asn Gln Phe Lys Asp Val 35 40 45 Phe Leu GlyPhe Asp Lys Arg Pro Tyr Ser Arg Ala Thr Thr Ala Gln 50 55 60 Lys Cys ValArg Ala Gly Gly Lys His Asn Asp Leu Glu Asn Val Gly 65 70 75 80 Tyr ThrAla Arg His His Thr Phe Phe Glu Met Met Gly Asn Phe Ser 85 90 95 Phe GlyAsp Tyr Phe Lys Arg Asp Ala Ile His Phe Ala Trp Glu Phe 100 105 110 LeuThr Ser Pro Glu Trp Leu Asn Ile Pro Lys Asp Lys Leu Leu Ala 115 120 125Thr Val Tyr Ala Glu Asp Asp Glu Ala Tyr Asn Ile Trp Leu Asn Glu 130 135140 Ile Gly Met Pro Ser Glu Arg Ile Val Arg Ile Gly Asp Asn Lys Gly 145150 155 160 Ala Lys Tyr Ala Ser Asp Asn Phe Trp Gln Met Gly Asp Thr GlyPro 165 170 175 Cys Gly Pro Cys Ser Glu Ile Phe Tyr Asp His Gly Glu GluIle Trp 180 185 190 Gly Gly Ile Pro Gly Ser Pro Glu Glu Asp Gly Asp ArgTrp Ile Glu 195 200 205 Ile Trp Asn Cys Val Phe Met Gln Phe Asn Arg AspGlu Gln Gly Asn 210 215 220 Met Asn Pro Leu Pro Lys Pro Ser Val Asp ThrGly Met Gly Leu Glu 225 230 235 240 Arg Ile Ala Ala Val Met Gln His ValHis Ser Asn Tyr Glu Ile Asp 245 250 255 Leu Phe Gln Asp Leu Leu Lys AlaVal Ala Arg Glu Thr Gly Ala Pro 260 265 270 Phe Arg Met Glu Glu Pro SerLeu Lys Val Ile Ala Asp His Ile Arg 275 280 285 Ser Cys Ser Phe Leu IleAla Asp Gly Val Leu Pro Ser Asn Glu Gly 290 295 300 Arg Gly Tyr Val LeuArg Arg Ile Ile Arg Arg Ala Val Arg His Gly 305 310 315 320 Tyr Lys LeuGly Gln Ser Lys Pro Phe Phe His Lys Leu Val Ala Asp 325 330 335 Leu ValLys Glu Met Gly Gly Ala Tyr Pro Glu Leu Lys Glu Lys Gln 340 345 350 AlaGln Ile Glu Glu Ala Leu Lys Asn Glu Glu Ser Arg Phe Ala Gln 355 360 365Thr Leu Glu Thr Gly Met Ala Leu Leu Glu Asn Ala Leu Val Lys Gly 370 375380 Gly Lys Thr Leu Gly Gly Glu Ile Ile Phe Lys Leu Tyr Asp Thr Tyr 385390 395 400 Gly Phe Pro Tyr Asp Leu Thr Ala Asp Ile Cys Arg Glu Arg AsnIle 405 410 415 Glu Pro Asp Glu Ala Gly Phe Glu Arg Glu Met Glu Ala GlnArg Ala 420 425 430 Arg Ala Arg Ala Ala Gln Ser Phe Lys Ala Asn Ala GlnLeu Pro Tyr 435 440 445 Asp Gly Gln Asp Thr Glu Phe Lys Gly Tyr Ser GluArg Gln Thr Glu 450 455 460 Ser Lys Val Leu Ala Leu Tyr Lys Asp Gly GluGln Val Asn Glu Leu 465 470 475 480 Asn Glu Gly Asp Ser Gly Ala Val ValIle Asp Phe Thr Pro Phe Tyr 485 490 495 Ala Glu Ser Gly Gly Gln Val GlyAsp Val Gly Tyr Ile Phe Ser Gly 500 505 510 Glu Asn Arg Phe Glu Val ArgAsp Thr Gln Lys Ile Lys Ala Ala Val 515 520 525 Phe Gly Gln Phe Gly ValGln Thr Ser Gly Arg Leu Lys Val Gly Asp 530 535 540 Ser Val Thr Ala LysVal Asp Asp Glu Ile Arg Asn Ala Asn Met Arg 545 550 555 560 Asn His SerAla Thr His Leu Met His Lys Ala Leu Arg Asp Val Leu 565 570 575 Gly ArgHis Val Glu Gln Lys Gly Ser Leu Val Thr Ala Glu Ser Thr 580 585 590 ArgPhe Asp Ile Ser His Pro Gln Ala Val Thr Ala Glu Glu Ile Ala 595 600 605Glu Val Glu Arg Arg Val Asn Glu Ala Ile Leu Ala Asn Val Ala Val 610 615620 Asn Ala Ala Ile Met Ser Met Glu Asp Ala Gln Lys Thr Gly Ala Met 625630 635 640 Met Leu Phe Gly Glu Lys Tyr Gly Glu Glu Val Arg Val Leu GlnMet 645 650 655 Gly Gly Phe Ser Thr Glu Leu Cys Gly Gly Thr His Val SerArg Thr 660 665 670 Gly Asp Ile Gly Leu Phe Lys Ile Ile Ser Glu Gly GlyIle Ala Ala 675 680 685 Gly Val Arg Arg Ile Glu Ala Ile Thr Gly Leu AsnAla Leu Lys Trp 690 695 700 Ala Gln Glu Gln Glu Arg Leu Val Lys Asp IleIle Ala Glu Thr Lys 705 710 715 720 Ala Gln Thr Glu Lys Asp Val Leu AlaLys Ile Gln Ala Gly Ala Ala 725 730 735 His Ala Lys Ala Leu Glu Lys GluLeu Ala Arg Ala Lys Ala Glu Leu 740 745 750 Ala Val His Ala Gly Ala LysLeu Leu Asp Asp Ala Lys Asp Leu Gly 755 760 765 Ala Ala Lys Leu Val AlaAla Gln Ile Glu Ala Asp Ala Ala Ala Leu 770 775 780 Arg Glu Ile Val ThrAsp Leu Thr Gly Lys Ser Asp Asn Ala Val Ile 785 790 795 800 Leu Leu AlaAla Val Asn Asp Gly Lys Val Ser Leu Cys Ala Gly Val 805 810 815 Ser LysPro Leu Thr Gly Lys Val Lys Ala Gly Asp Leu Val Lys Phe 820 825 830 AlaAla Glu Gln Val Gly Gly Lys Gly Gly Gly Arg Pro Asp Leu Ala 835 840 845Gln Ala Gly Gly Thr Asp Ala Asp Lys Leu Pro Ala Val Leu Asp Ser 850 855860 Val Lys Asp Trp Val Gly Ala Lys Leu Val 865 870 33 774 DNA Neisseriameningitidis CDS (1)..(774) NMA0750 33 atg gct aag aaa ttc gat aaa gacagc ttc agc ggc acg ctg att gtc 48 Met Ala Lys Lys Phe Asp Lys Asp SerPhe Ser Gly Thr Leu Ile Val 1 5 10 15 gtg ttg gcg gtc agc ctg att tgctcg gtc atc gtt gcc ggt gcg gtc 96 Val Leu Ala Val Ser Leu Ile Cys SerVal Ile Val Ala Gly Ala Val 20 25 30 gtc ggc ttg aaa ccc atc caa gag aaacaa aaa ctc caa gac aaa caa 144 Val Gly Leu Lys Pro Ile Gln Glu Lys GlnLys Leu Gln Asp Lys Gln 35 40 45 ggc tat atc ttg agc gta gcc ggt ttg atggat aag gac acc gac atc 192 Gly Tyr Ile Leu Ser Val Ala Gly Leu Met AspLys Asp Thr Asp Ile 50 55 60 ggt aaa act ttt gcc gag cgt atc gag caa cgcgtg gtc gat ttg gcg 240 Gly Lys Thr Phe Ala Glu Arg Ile Glu Gln Arg ValVal Asp Leu Ala 65 70 75 80 acc ggc gaa tat gtg aaa gac gcg ccg aaa gacttc agt gcg cgc atc 288 Thr Gly Glu Tyr Val Lys Asp Ala Pro Lys Asp PheSer Ala Arg Ile 85 90 95 gca ggc aaa gac ccc gcg caa agc atc cgc atc aaaccc gaa gac gat 336 Ala Gly Lys Asp Pro Ala Gln Ser Ile Arg Ile Lys ProGlu Asp Asp 100 105 110 ttg gca ggc atc aaa agc cgt gcc aaa tac acc gaggtt tac ttg gta 384 Leu Ala Gly Ile Lys Ser Arg Ala Lys Tyr Thr Glu ValTyr Leu Val 115 120 125 aaa ggt gaa gac ggc aaa atc ggc caa atc atc ctgcct atg cac ggt 432 Lys Gly Glu Asp Gly Lys Ile Gly Gln Ile Ile Leu ProMet His Gly 130 135 140 aac ggt ttg tgg tcg gtc atg tac ggc ttt gtc gccatc caa ccc gac 480 Asn Gly Leu Trp Ser Val Met Tyr Gly Phe Val Ala IleGln Pro Asp 145 150 155 160 ggc aac acc atc aac ggc att acc tac tac gagcaa ggc gaa acc ccg 528 Gly Asn Thr Ile Asn Gly Ile Thr Tyr Tyr Glu GlnGly Glu Thr Pro 165 170 175 ggc tta ggc ggc gaa atc ggc aat cct ttg tggcag caa aaa ttc gtc 576 Gly Leu Gly Gly Glu Ile Gly Asn Pro Leu Trp GlnGln Lys Phe Val 180 185 190 ggc aaa aaa ctg ttt gac gga caa ggc aaa ctcgcc ctg cac gtc ggc 624 Gly Lys Lys Leu Phe Asp Gly Gln Gly Lys Leu AlaLeu His Val Gly 195 200 205 aaa ggc gcg ggt tcg gac aaa gaa cac ggc gtagat gcc ctc tcc ggc 672 Lys Gly Ala Gly Ser Asp Lys Glu His Gly Val AspAla Leu Ser Gly 210 215 220 gcg tcg ctg aca tcc aaa ggc gta caa ggt tcgttc gcc tac tgg ttc 720 Ala Ser Leu Thr Ser Lys Gly Val Gln Gly Ser PheAla Tyr Trp Phe 225 230 235 240 ggc gaa aac ggc tat atc ccc tac ctg aacaaa ttg aaa tca gca gga 768 Gly Glu Asn Gly Tyr Ile Pro Tyr Leu Asn LysLeu Lys Ser Ala Gly 245 250 255 gcg caa 774 Ala Gln 34 258 PRT Neisseriameningitidis 34 Met Ala Lys Lys Phe Asp Lys Asp Ser Phe Ser Gly Thr LeuIle Val 1 5 10 15 Val Leu Ala Val Ser Leu Ile Cys Ser Val Ile Val AlaGly Ala Val 20 25 30 Val Gly Leu Lys Pro Ile Gln Glu Lys Gln Lys Leu GlnAsp Lys Gln 35 40 45 Gly Tyr Ile Leu Ser Val Ala Gly Leu Met Asp Lys AspThr Asp Ile 50 55 60 Gly Lys Thr Phe Ala Glu Arg Ile Glu Gln Arg Val ValAsp Leu Ala 65 70 75 80 Thr Gly Glu Tyr Val Lys Asp Ala Pro Lys Asp PheSer Ala Arg Ile 85 90 95 Ala Gly Lys Asp Pro Ala Gln Ser Ile Arg Ile LysPro Glu Asp Asp 100 105 110 Leu Ala Gly Ile Lys Ser Arg Ala Lys Tyr ThrGlu Val Tyr Leu Val 115 120 125 Lys Gly Glu Asp Gly Lys Ile Gly Gln IleIle Leu Pro Met His Gly 130 135 140 Asn Gly Leu Trp Ser Val Met Tyr GlyPhe Val Ala Ile Gln Pro Asp 145 150 155 160 Gly Asn Thr Ile Asn Gly IleThr Tyr Tyr Glu Gln Gly Glu Thr Pro 165 170 175 Gly Leu Gly Gly Glu IleGly Asn Pro Leu Trp Gln Gln Lys Phe Val 180 185 190 Gly Lys Lys Leu PheAsp Gly Gln Gly Lys Leu Ala Leu His Val Gly 195 200 205 Lys Gly Ala GlySer Asp Lys Glu His Gly Val Asp Ala Leu Ser Gly 210 215 220 Ala Ser LeuThr Ser Lys Gly Val Gln Gly Ser Phe Ala Tyr Trp Phe 225 230 235 240 GlyGlu Asn Gly Tyr Ile Pro Tyr Leu Asn Lys Leu Lys Ser Ala Gly 245 250 255Ala Gln 35 624 DNA Neisseria meningitidis CDS (1)..(624) NMA0749 35 atggct gat atg aaa cgc ttg aaa cat ttg atg ttt tca ccc ttt atc 48 Met AlaAsp Met Lys Arg Leu Lys His Leu Met Phe Ser Pro Phe Ile 1 5 10 15 gacaac aac ccg att gcc ttg cag gtt ttg ggt att tgt tcg gct ttg 96 Asp AsnAsn Pro Ile Ala Leu Gln Val Leu Gly Ile Cys Ser Ala Leu 20 25 30 gcg gttacc acc aaa ctt cag acg gcc atc gtg atg ggt att tcc gta 144 Ala Val ThrThr Lys Leu Gln Thr Ala Ile Val Met Gly Ile Ser Val 35 40 45 gct ttg gtaacc ggt ttt tcc agc ttc ttc att tcg ctg gta cgc aac 192 Ala Leu Val ThrGly Phe Ser Ser Phe Phe Ile Ser Leu Val Arg Asn 50 55 60 tac atc ccc aacagc atc cgc atc atc gtg caa atg gcg att atc gcg 240 Tyr Ile Pro Asn SerIle Arg Ile Ile Val Gln Met Ala Ile Ile Ala 65 70 75 80 tcg ctg gtt acgctg gtc gac caa ctc ttg cag gca ttt gcc tac gaa 288 Ser Leu Val Thr LeuVal Asp Gln Leu Leu Gln Ala Phe Ala Tyr Glu 85 90 95 ttg tcc aaa cag ctttcc gta ttc gtc ggt ctg att att acc aac tgt 336 Leu Ser Lys Gln Leu SerVal Phe Val Gly Leu Ile Ile Thr Asn Cys 100 105 110 atc gtg atg ggc cgcgcc gaa gca ttt gcg atg aaa gag ccg ccg ctg 384 Ile Val Met Gly Arg AlaGlu Ala Phe Ala Met Lys Glu Pro Pro Leu 115 120 125 gaa agc ctg atc gacggc atc ggc aac ggc gcg ggc tac ggg atg atg 432 Glu Ser Leu Ile Asp GlyIle Gly Asn Gly Ala Gly Tyr Gly Met Met 130 135 140 ctg ctt gtc gtc gccacc gtc cgc gaa ctg att ggc tcg ggc aaa ctc 480 Leu Leu Val Val Ala ThrVal Arg Glu Leu Ile Gly Ser Gly Lys Leu 145 150 155 160 ttg ggc tac accgtt ttc caa acc gtg cag gac ggc ggc tgg tat cag 528 Leu Gly Tyr Thr ValPhe Gln Thr Val Gln Asp Gly Gly Trp Tyr Gln 165 170 175 acc aac ggc ttgttc ctg ctc gcc ccc agc gcg ttc ttc atc atc ggc 576 Thr Asn Gly Leu PheLeu Leu Ala Pro Ser Ala Phe Phe Ile Ile Gly 180 185 190 ttt ttg att tgggga ctg cgt acc tgg aaa ccc gaa cag gcg gag gaa 624 Phe Leu Ile Trp GlyLeu Arg Thr Trp Lys Pro Glu Gln Ala Glu Glu 195 200 205 36 208 PRTNeisseria meningitidis 36 Met Ala Asp Met Lys Arg Leu Lys His Leu MetPhe Ser Pro Phe Ile 1 5 10 15 Asp Asn Asn Pro Ile Ala Leu Gln Val LeuGly Ile Cys Ser Ala Leu 20 25 30 Ala Val Thr Thr Lys Leu Gln Thr Ala IleVal Met Gly Ile Ser Val 35 40 45 Ala Leu Val Thr Gly Phe Ser Ser Phe PheIle Ser Leu Val Arg Asn 50 55 60 Tyr Ile Pro Asn Ser Ile Arg Ile Ile ValGln Met Ala Ile Ile Ala 65 70 75 80 Ser Leu Val Thr Leu Val Asp Gln LeuLeu Gln Ala Phe Ala Tyr Glu 85 90 95 Leu Ser Lys Gln Leu Ser Val Phe ValGly Leu Ile Ile Thr Asn Cys 100 105 110 Ile Val Met Gly Arg Ala Glu AlaPhe Ala Met Lys Glu Pro Pro Leu 115 120 125 Glu Ser Leu Ile Asp Gly IleGly Asn Gly Ala Gly Tyr Gly Met Met 130 135 140 Leu Leu Val Val Ala ThrVal Arg Glu Leu Ile Gly Ser Gly Lys Leu 145 150 155 160 Leu Gly Tyr ThrVal Phe Gln Thr Val Gln Asp Gly Gly Trp Tyr Gln 165 170 175 Thr Asn GlyLeu Phe Leu Leu Ala Pro Ser Ala Phe Phe Ile Ile Gly 180 185 190 Phe LeuIle Trp Gly Leu Arg Thr Trp Lys Pro Glu Gln Ala Glu Glu 195 200 205 37630 DNA Neisseria meningitidis CDS (1)..(630) NMA0387 37 atg acc cta cgttac gaa atc ctc tcc gtt acc ccc ttc cgc caa aac 48 Met Thr Leu Arg TyrGlu Ile Leu Ser Val Thr Pro Phe Arg Gln Asn 1 5 10 15 tgc acc ctg atttgg gac gac gaa agc ggc gaa gcc gtc ctg acc gat 96 Cys Thr Leu Ile TrpAsp Asp Glu Ser Gly Glu Ala Val Leu Thr Asp 20 25 30 gtc ggc ggc gac gtgccg ttc ctg ctg caa gcg ttg gca aac cgc aaa 144 Val Gly Gly Asp Val ProPhe Leu Leu Gln Ala Leu Ala Asn Arg Lys 35 40 45 ctt acg ctc acg gca atctgg ctg acg cac ggc cat ctc gat cac gcg 192 Leu Thr Leu Thr Ala Ile TrpLeu Thr His Gly His Leu Asp His Ala 50 55 60 ggc ggc gtg gtc gaa atg ttgaaa acg cat aaa gtc cct gtc ctc ggg 240 Gly Gly Val Val Glu Met Leu LysThr His Lys Val Pro Val Leu Gly 65 70 75 80 ccg cat ccg gac gat gaa ttcctg ctc caa tcg ctg ccg caa acc acc 288 Pro His Pro Asp Asp Glu Phe LeuLeu Gln Ser Leu Pro Gln Thr Thr 85 90 95 gcg caa tac gga ttt cct gtc tcgccc gcc ttt gcg ccg aac cgt tgg 336 Ala Gln Tyr Gly Phe Pro Val Ser ProAla Phe Ala Pro Asn Arg Trp 100 105 110 ctc gaa gaa ggc gaa acg ctc acggtc gga cgc tat gcc ttt caa gtg 384 Leu Glu Glu Gly Glu Thr Leu Thr ValGly Arg Tyr Ala Phe Gln Val 115 120 125 ctg cat att ccg ggc cat acg ccggga cat atc gtc ttt tat tgt gcc 432 Leu His Ile Pro Gly His Thr Pro GlyHis Ile Val Phe Tyr Cys Ala 130 135 140 gag gcg gaa ttg ctg att gcg ggcgac gtg ctg ttt tac gaa acc ata 480 Glu Ala Glu Leu Leu Ile Ala Gly AspVal Leu Phe Tyr Glu Thr Ile 145 150 155 160 ggc aga acc gat ttt ccg cgcggc aac cac gcc gac tta atc aat aat 528 Gly Arg Thr Asp Phe Pro Arg GlyAsn His Ala Asp Leu Ile Asn Asn 165 170 175 atc cgc aac aaa tta ttc gccctc ccc gaa acc gtg caa gtt gtc gcc 576 Ile Arg Asn Lys Leu Phe Ala LeuPro Glu Thr Val Gln Val Val Ala 180 185 190 gga cac ggg cgt atg act tccatc gga cac gaa aaa cgg cac aat ccg 624 Gly His Gly Arg Met Thr Ser IleGly His Glu Lys Arg His Asn Pro 195 200 205 ttt ttc 630 Phe Phe 210 402514 PRT Neisseria meningitidis 40 Met Asn Lys Thr Leu Tyr Arg Val IlePhe Asn Arg Lys Arg Gly Ala 1 5 10 15 Val Val Ala Val Ala Glu Thr ThrLys Arg Glu Gly Lys Ser Cys Ala 20 25 30 Asp Ser Asp Ser Gly Ser Ala HisVal Lys Ser Val Pro Phe Gly Thr 35 40 45 Thr His Ala Pro Val Cys Arg SerAsn Ile Phe Ser Phe Ser Leu Leu 50 55 60 Gly Phe Ser Leu Cys Leu Ala ValGly Thr Ala Asn Ile Ala Phe Ala 65 70 75 80 Asp Gly Ile Ile Ala Asp LysAla Ala Pro Lys Thr Gln Gln Ala Thr 85 90 95 Ile Leu Gln Thr Gly Asn GlyIle Pro Gln Val Asn Ile Gln Thr Pro 100 105 110 Thr Ser Ala Gly Val SerVal Asn Gln Tyr Ala Gln Phe Asp Val Gly 115 120 125 Asn Arg Gly Ala IleLeu Asn Asn Ser Arg Ser Asn Thr Gln Thr Gln 130 135 140 Leu Gly Gly TrpIle Gln Gly Asn Pro Trp Leu Ala Arg Gly Glu Ala 145 150 155 160 Arg ValVal Val Asn Gln Ile Asn Ser Ser His Ser Ser Gln Met Asn 165 170 175 GlyTyr Ile Glu Val Gly Gly Arg Arg Ala Glu Val Val Ile Ala Asn 180 185 190Pro Ala Gly Ile Ala Val Asn Gly Gly Gly Phe Ile Asn Ala Ser Arg 195 200205 Ala Thr Leu Thr Thr Gly Gln Pro Gln Tyr Gln Ala Gly Asp Leu Ser 210215 220 Gly Phe Lys Ile Arg Gln Gly Asn Val Val Ile Ala Gly His Gly Leu225 230 235 240 Asp Ala Arg Asp Thr Asp Phe Thr Arg Ile Leu Ser Tyr HisSer Lys 245 250 255 Ile Asp Ala Pro Val Trp Gly Gln Asp Val Arg Val ValAla Gly Gln 260 265 270 Asn Asp Val Val Ala Thr Gly Asn Ala His Ser ProIle Leu Asn Asn 275 280 285 Ala Ala Ala Asn Thr Ser Asn Asn Thr Ala AsnAsn Gly Thr His Ile 290 295 300 Pro Leu Phe Ala Ile Asp Thr Gly Lys LeuGly Gly Met Tyr Ala Asn 305 310 315 320 Lys Ile Thr Leu Ile Ser Thr AlaGlu Gln Ala Gly Ile Arg Asn Gln 325 330 335 Gly Gln Leu Phe Ala Ser SerGly Asn Val Ala Ile Asp Ala Asn Gly 340 345 350 Arg Leu Val Asn Ser GlyThr Met Ala Ala Ala Asn Ala Lys Asp Thr 355 360 365 Asp Asn Thr Ala GluHis Lys Val Asn Ile Arg Ser Gln Gly Val Glu 370 375 380 Asn Ser Gly ThrAla Val Ser Gln Gln Gly Thr Gln Ile His Ser Gln 385 390 395 400 Ser IleGln Asn Thr Gly Thr Leu Leu Ser Ser Gly Glu Ile Leu Ile 405 410 415 HisAsn Ser Gly Ser Leu Lys Asn Glu Thr Ser Gly Thr Ile Glu Ala 420 425 430Ala Arg Leu Ala Ile Asp Thr Asp Thr Leu Asn Asn Gln Gly Lys Leu 435 440445 Ser Gln Thr Gly Ser Gln Lys Leu His Ile Asp Ala Gln Gly Lys Met 450455 460 Asp Asn Arg Gly Arg Met Gly Leu Gln Asp Thr Ala Pro Thr Ala Ser465 470 475 480 Asn Gly Ser Ser Asn Gln Thr Gly Asn Ser Tyr Asn Ala SerPhe His 485 490 495 Ser Ser Thr Thr Thr Pro Thr Thr Ala Thr Gly Thr GlyThr Ala Thr 500 505 510 Val Ser Ile Ser Asn Ile Thr Ala Pro Thr Phe AlaAsp Gly Thr Ile 515 520 525 Arg Thr His Gly Ala Leu Asp Asn Ser Gly SerIle Ile Ala Asn Gly 530 535 540 Gln Thr Asp Val Ser Ala Gln Gln Gly LeuAsn Asn Ala Gly Gln Ile 545 550 555 560 Asp Ile His Gln Leu Asn Ala LysGly Ser Ala Phe Asp Asn His Asn 565 570 575 Gly Thr Ile Ile Ser Asp AlaVal His Ile Gln Ala Gly Ser Leu Asn 580 585 590 Asn Gln Asn Gly Asn IleThr Thr Arg Gln Gln Leu Glu Ile Glu Thr 595 600 605 Asp Gln Leu Asp AsnAla His Gly Lys Leu Leu Ser Ala Glu Ile Ala 610 615 620 Asp Leu Ala ValSer Gly Ser Leu Asn Asn Gln Asn Gly Glu Ile Ala 625 630 635 640 Thr AsnGln Gln Leu Ile Ile His Asp Gly Gln Gln Ser Thr Ala Val 645 650 655 IleAsp Asn Thr Asn Gly Thr Ile Gln Ser Gly Arg Asp Val Ala Ile 660 665 670Gln Ala Lys Ser Leu Ser Asn Asn Gly Thr Leu Ala Ala Asp Asn Lys 675 680685 Leu Asp Ile Ala Leu Gln Asp Asp Phe Tyr Val Glu Arg Asn Ile Val 690695 700 Ala Gly Asn Glu Leu Ser Leu Ser Thr Arg Gly Ser Leu Lys Asn Ser705 710 715 720 His Thr Leu Gln Ala Gly Lys Arg Ile Arg Ile Lys Ala AsnAsn Leu 725 730 735 Asp Asn Ala Ala Gln Gly Asn Ile Gln Ser Gly Gly ThrThr Asp Ile 740 745 750 Gly Thr Gln His Asn Leu Thr Asn Arg Gly Leu IleAsp Gly Gln Gln 755 760 765 Thr Lys Ile Gln Ala Gly Gln Met Asn Asn IleGly Thr Gly Arg Ile 770 775 780 Tyr Gly Asp Asn Ile Ala Ile Ala Ala ThrArg Leu Asp Asn Gln Asp 785 790 795 800 Glu Asn Gly Thr Gly Ala Ala IleAla Ala Arg Glu Asn Leu Asn Leu 805 810 815 Gly Ile Gly Gln Leu Asn AsnArg Glu Asn Ser Leu Ile Tyr Ser Gly 820 825 830 Asn Asp Met Ala Val GlyGly Ala Leu Asp Thr Asn Gly Gln Ala Thr 835 840 845 Gly Lys Ala Gln ArgIle His Asn Ala Gly Ala Thr Ile Glu Ala Ala 850 855 860 Gly Lys Met ArgLeu Gly Val Glu Lys Leu His Asn Thr Asn Glu His 865 870 875 880 Leu LysThr Gln Leu Val Glu Thr Gly Arg Glu His Ile Val Asp Tyr 885 890 895 GluAla Phe Gly Arg His Glu Leu Leu Arg Glu Gly Thr Gln His Glu 900 905 910Leu Gly Trp Ser Val Tyr Asn Asp Glu Ser Asp His Leu Arg Thr Pro 915 920925 Asp Gly Ala Ala His Glu Asn Trp His Lys Tyr Asp Tyr Glu Lys Val 930935 940 Thr Gln Lys Thr Gln Val Thr Gln Thr Ala Pro Ala Lys Ile Ile Ser945 950 955 960 Gly Asn Asp Leu Thr Ile Asp Gly Lys Glu Val Phe Asn ThrAsp Ser 965 970 975 Gln Ile Ile Ala Gly Gly Asn Leu Ile Val Gln Thr GluLys Asp Gly 980 985 990 Leu His Asn Glu Gln Thr Phe Gly Glu Lys Lys ValPhe Ser Glu Asn 995 1000 1005 Gly Lys Leu His Ser Tyr Trp Arg Glu LysHis Lys Gly Arg Asp 1010 1015 1020 Ser Thr Gly His Ser Glu Gln Asn TyrThr Leu Pro Glu Glu Ile 1025 1030 1035 Thr Arg Asn Ile Ser Leu Gly SerPhe Ala Tyr Glu Ser His Arg 1040 1045 1050 Lys Ala Leu Ser His His AlaPro Ser Gln Gly Thr Glu Leu Pro 1055 1060 1065 Gln Ser Asn Gly Ile SerLeu Pro Tyr Thr Ser Asn Ser Phe Thr 1070 1075 1080 Pro Leu Pro Ser SerSer Leu Tyr Ile Ile Asn Pro Val Asn Lys 1085 1090 1095 Gly Tyr Leu ValGlu Thr Asp Pro Arg Phe Ala Asn Tyr Arg Gln 1100 1105 1110 Trp Leu GlySer Asp Tyr Met Leu Asp Ser Leu Lys Leu Asp Pro 1115 1120 1125 Asn AsnLeu His Lys Arg Leu Gly Asp Gly Tyr Tyr Glu Gln Arg 1130 1135 1140 LeuIle Asn Glu Gln Ile Ala Glu Leu Thr Gly His Arg Arg Leu 1145 1150 1155Asp Gly Tyr Gln Asn Asp Glu Glu Gln Phe Lys Ala Leu Met Asp 1160 11651170 Asn Gly Ala Thr Ala Ala Arg Ser Met Asn Leu Ser Val Gly Ile 11751180 1185 Ala Leu Ser Ala Glu Gln Val Ala Gln Leu Thr Ser Asp Ile Val1190 1195 1200 Trp Leu Val Gln Lys Glu Val Lys Leu Pro Asp Gly Gly ThrGln 1205 1210 1215 Thr Val Leu Val Pro Gln Val Tyr Val Arg Val Lys AsnGly Asp 1220 1225 1230 Ile Asp Gly Lys Gly Ala Leu Leu Ser Gly Ser AsnThr Gln Ile 1235 1240 1245 Asn Val Ser Gly Ser Leu Lys Asn Ser Gly ThrIle Ala Gly Arg 1250 1255 1260 Asn Ala Leu Ile Ile Asn Thr Asp Thr LeuAsp Asn Ile Gly Gly 1265 1270 1275 Arg Ile His Ala Gln Lys Ser Ala ValThr Ala Thr Gln Asp Ile 1280 1285 1290 Asn Asn Ile Gly Gly Met Leu SerAla Glu Gln Thr Leu Leu Leu 1295 1300 1305 Asn Ala Gly Asn Asn Ile AsnSer Gln Ser Thr Thr Ala Ser Ser 1310 1315 1320 Gln Asn Thr Gln Gly SerSer Thr Tyr Leu Asp Arg Met Ala Gly 1325 1330 1335 Ile Tyr Ile Thr GlyLys Glu Lys Gly Val Leu Ala Ala Gln Ala 1340 1345 1350 Gly Lys Asp IleAsn Ile Ile Ala Gly Gln Ile Ser Asn Gln Ser 1355 1360 1365 Glu Gln GlyGln Thr Arg Leu Gln Ala Gly Arg Asp Ile Asn Leu 1370 1375 1380 Asp ThrVal Gln Thr Ser Lys His Gln Ala Thr His Phe Asp Ala 1385 1390 1395 AspAsn His Val Ile Arg Gly Ser Thr Asn Glu Val Gly Ser Ser 1400 1405 1410Ile Gln Thr Lys Gly Asp Val Thr Leu Leu Ser Gly Asn Asn Leu 1415 14201425 Asn Ala Lys Ala Ala Glu Val Ser Ser Ala Asn Gly Thr Leu Ala 14301435 1440 Val Ser Ala Lys Asn Asp Ile Asn Ile Ser Ala Gly Ile Asn Thr1445 1450 1455 Thr His Val Asp Asp Ala Ser Lys His Thr Gly Arg Ser GlyGly 1460 1465 1470 Gly Asn Lys Leu Val Ile Thr Asp Lys Ala Gln Ser HisHis Glu 1475 1480 1485 Thr Ala Gln Ser Ser Thr Phe Glu Gly Lys Gln ValVal Leu Gln 1490 1495 1500 Ala Gly Asn Asp Ala Asn Ile Leu Gly Ser AsnVal Ile Ser Asp 1505 1510 1515 Asn Gly Thr Gln Ile Gln Ala Gly Asn HisVal Arg Ile Gly Thr 1520 1525 1530 Thr Gln Thr Gln Ser Gln Ser Glu ThrTyr His Gln Thr Gln Lys 1535 1540 1545 Ser Gly Leu Met Ser Ala Gly IleGly Phe Thr Ile Gly Ser Lys 1550 1555 1560 Thr Asn Thr Gln Glu Asn GlnSer Gln Ser Asn Glu His Thr Gly 1565 1570 1575 Ser Thr Val Gly Ser LeuLys Gly Asp Thr Thr Ile Val Ala Gly 1580 1585 1590 Lys His Tyr Glu GlnIle Gly Ser Thr Val Ser Ser Pro Glu Gly 1595 1600 1605 Asn Asn Thr IleTyr Ala Gln Ser Ile Asp Ile Gln Ala Ala His 1610 1615 1620 Asn Lys LeuAsn Ser Asn Thr Thr Gln Thr Tyr Glu Gln Lys Gly 1625 1630 1635 Leu ThrVal Ala Phe Ser Ser Pro Val Thr Asp Leu Ala Gln Gln 1640 1645 1650 AlaIle Ala Val Ala Gln Ser Ser Lys Gln Val Gly Gln Ser Lys 1655 1660 1665Asn Asp Arg Val Asn Ala Met Ala Ala Ala Asn Ala Gly Trp Gln 1670 16751680 Ala Tyr Gln Thr Gly Lys Ser Ala Gln Asn Leu Ala Asn Gly Thr 16851690 1695 Thr Asn Ala Lys Gln Val Ser Ile Ser Ile Thr Tyr Gly Glu Gln1700 1705 1710 Gln Asn Arg Gln Thr Thr Gln Val Gln Ala Asn Gln Ala GlnAla 1715 1720 1725 Ser Gln Ile Gln Ala Gly Gly Lys Thr Thr Leu Ile AlaThr Gly 1730 1735 1740 Ala Ala Glu Gln Ser Asn Ile Asn Ile Ala Gly SerAsp Val Ala 1745 1750 1755 Gly Lys Ala Gly Thr Ile Leu Ile Ala Asp AsnAsp Ile Thr Leu 1760 1765 1770 Gln Ser Ala Glu Gln Ser Asn Thr Glu ArgGly Gln Asn Lys Ser 1775 1780 1785 Ala Gly Trp Asn Ala Gly Ala Ala ValSer Phe Gly Gln Gly Gly 1790 1795 1800 Trp Ser Leu Gly Val Thr Ala GlyGly Asn Val Gly Lys Gly Tyr 1805 1810 1815 Gly Asn Gly Asp Ser Ile ThrHis Arg His Ser His Ile Gly Asp 1820 1825 1830 Lys Gly Ser Gln Thr LeuIle Gln Ser Gly Gly Asp Thr Thr Ile 1835 1840 1845 Lys Gly Ala Gln ValArg Gly Lys Gly Val Gln Val Asn Ala Lys 1850 1855 1860 Asn Leu Ser IleGln Ser Val Gln Asp Arg Glu Thr Tyr Gln Ser 1865 1870 1875 Lys Gln GlnAsn Ala Ser Ala Gln Val Thr Val Gly Tyr Gly Phe 1880 1885 1890 Ser AlaGly Gly Asp Tyr Ser Gln Ser Lys Ile Arg Ala Asp His 1895 1900 1905 ValSer Val Thr Glu Gln Ser Gly Ile Tyr Ala Gly Glu Asp Gly 1910 1915 1920Tyr Gln Ile Lys Val Gly Asn His Thr Asp Leu Lys Gly Gly Ile 1925 19301935 Ile Thr Ser Thr Gln Ser Ala Glu Asp Lys Gly Lys Asn Arg Phe 19401945 1950 Gln Thr Ala Thr Leu Thr His Ser Asp Ile Lys Asn His Ser Gln1955 1960 1965 Tyr Lys Gly Glu Ser Phe Gly Leu Gly Ala Ser Ala Ser IleSer 1970 1975 1980 Gly Lys Thr Leu Gly Gln Gly Ala Gln Asn Lys Pro GlnAsn Lys 1985 1990 1995 His Leu Thr Ser Val Ala Asp Lys Asn Ser Ala SerSer Ser Val 2000 2005 2010 Gly Tyr Gly Ser Asp Ser Asp Ser Gln Ser SerIle Thr Lys Ser 2015 2020 2025 Gly Ile Asn Thr Arg Asn Ile Gln Ile ThrAsp Glu Ala Ala Gln 2030 2035 2040 Ile Arg Leu Thr Gly Lys Thr Ala AlaGln Thr Lys Ala Asp Ile 2045 2050 2055 Asp Thr Asn Val Thr Thr Asp ThrAla Glu Arg His Ser Gly Ser 2060 2065 2070 Leu Lys Asn Thr Phe Asn LysGlu Ala Val Gln Ser Glu Leu Asp 2075 2080 2085 Leu Gln Arg Thr Val SerGln Asp Phe Ser Lys Asn Val Gln Gln 2090 2095 2100 Ala Asn Thr Glu IleAsn Gln His Leu Asp Lys Leu Lys Ala Asp 2105 2110 2115 Lys Glu Ala AlaGlu Thr Ala Ala Ala Glu Ala Leu Ala Asn Gly 2120 2125 2130 Asp Met GluThr Ala Lys Arg Lys Ala His Glu Ala Gln Asp Ala 2135 2140 2145 Ala AlaLys Ala Asp Asn Trp Gln Gln Gly Lys Val Ile Leu Asn 2150 2155 2160 MetLeu Ala Ser Gly Leu Ala Ala Pro Thr Gln Ser Gly Ala Gly 2165 2170 2175Ile Ala Ala Ala Thr Ala Ser Pro Ala Val Ser Tyr Ala Ile Gly 2180 21852190 Gln His Phe Lys Asp Leu Ala Gly Gln Asn Ala Asn Gly Lys Leu 21952200 2205 Thr Ala Ser Gln Glu Thr Ala His Val Leu Ala His Ala Val Leu2210 2215 2220 Gly Ala Ala Val Ala Ala Val Gly Asp Asn Asn Ala Leu AlaGly 2225 2230 2235 Ala Leu Ser Ala Gly Gly Ser Glu Ala Ala Ala Pro TyrIle Ser 2240 2245 2250 Lys Trp Leu Tyr Gly Lys Glu Lys Gly Ser Asp LeuThr Ala Glu 2255 2260 2265 Glu Lys Glu Thr Val Thr Ala Ile Thr Asn ValLeu Gly Thr Ala 2270 2275 2280 Thr Gly Ala Ala Val Gly Asn Ser Ala ThrAsp Ala Ala Gln Gly 2285 2290 2295 Ser Leu Asn Ala Gln Ser Ala Val GluAsn Asn Asp Thr Val Glu 2300 2305 2310 Gln Val Lys Phe Ala Leu Arg HisPro Arg Ile Ala Ile Ala Ile 2315 2320 2325 Gly Ser Val His Lys Asp ProGly Ser Thr Leu Glu Pro Asn Ile 2330 2335 2340 Ser Thr Ile Ala Ser ThrPhe Gln Leu Asn Leu Phe Pro Asn Ser 2345 2350 2355 Glu Phe Gly Gly GluGly Gly Val Gly Asn Ala Phe Arg His Val 2360 2365 2370 Leu Trp Gln AlaThr Ile Thr Arg Glu Phe Gly Lys Asp Ile Ala 2375 2380 2385 Val Lys ValGly Asn Ser His Glu Ser Gly Glu Lys Ile Asn Tyr 2390 2395 2400 Ser IleArg Arg Asn Leu Ser Leu Asp Lys Ala Asp Glu Met Ile 2405 2410 2415 AspGln Leu Asn Asn Glu Ile Gly Arg Glu Ile Ala Leu Asn Thr 2420 2425 2430Asn Arg Leu Asn Thr Lys Glu Leu Val Gly Leu Ile Leu Glu Thr 2435 24402445 Tyr Lys Asn Asn Gly Phe Tyr Gln Ala Glu Arg Asn Ser Asn Gly 24502455 2460 Asn Tyr Asp Val Val Arg Lys Arg Leu Ser Glu Lys Asp Tyr Gln2465 2470 2475 Asn Thr Ser Asn Ile Leu Ile His Leu Asp Asn Thr Gly AlaGly 2480 2485 2490 Phe Lys Ile Gln Gln Arg Arg Lys Gln Ile Arg Ala GlnIle Ser 2495 2500 2505 Ala Arg Gln Trp Arg Arg 2510 41 1275 DNANeisseria meningitidis CDS (1)..(1275) NMB0115 41 atg cgt agc agc gatatt tta att gta gac gac gaa atc ggc atc cgc 48 Met Arg Ser Ser Asp IleLeu Ile Val Asp Asp Glu Ile Gly Ile Arg 1 5 10 15 gac ctg ctg tcg gaaatc ctg cag gac gaa ggt tat tcg gtc gca ttg 96 Asp Leu Leu Ser Glu IleLeu Gln Asp Glu Gly Tyr Ser Val Ala Leu 20 25 30 gcg gaa aac gcc gaa gaggcg cgc aag ctg cgc cat cag gcg cgc ccc 144 Ala Glu Asn Ala Glu Glu AlaArg Lys Leu Arg His Gln Ala Arg Pro 35 40 45 gcg atg gtg ctg ctg gat atttgg atg cct gat tgc gac ggc atc acc 192 Ala Met Val Leu Leu Asp Ile TrpMet Pro Asp Cys Asp Gly Ile Thr 50 55 60 ctt ttg aag gag tgg gcg aaa aacggg cag ctc aat atg ccg gtg gtg 240 Leu Leu Lys Glu Trp Ala Lys Asn GlyGln Leu Asn Met Pro Val Val 65 70 75 80 atg atg agc ggg cat gcc agc atcgat acc gcc gtg gaa gcc acc aaa 288 Met Met Ser Gly His Ala Ser Ile AspThr Ala Val Glu Ala Thr Lys 85 90 95 atc ggc gcg atc gat ttt ttg gaa aaaccg att tcc ctg caa aag ctg 336 Ile Gly Ala Ile Asp Phe Leu Glu Lys ProIle Ser Leu Gln Lys Leu 100 105 110 ctg tct gcc gtc gaa aac gcg ttg aagtac ggt gcg gcg caa acc gaa 384 Leu Ser Ala Val Glu Asn Ala Leu Lys TyrGly Ala Ala Gln Thr Glu 115 120 125 acg ggg cct gta ttc gac aag ctg ggcaac agt gcg gcg att cag gaa 432 Thr Gly Pro Val Phe Asp Lys Leu Gly AsnSer Ala Ala Ile Gln Glu 130 135 140 atg aac cgt gag gta ggg gct gcg gtgaaa tgt gcc tct ccc gta ctt 480 Met Asn Arg Glu Val Gly Ala Ala Val LysCys Ala Ser Pro Val Leu 145 150 155 160 ttg acg ggc gag gcg ggt tcg ccgttt gaa acg gtg gca cgc tat ttc 528 Leu Thr Gly Glu Ala Gly Ser Pro PheGlu Thr Val Ala Arg Tyr Phe 165 170 175 cat aaa aac ggt acg ccg tgg gtcagc ccg gca agg gtc gaa tat ctg 576 His Lys Asn Gly Thr Pro Trp Val SerPro Ala Arg Val Glu Tyr Leu 180 185 190 atc gat atg ccg atg gaa ctg ttgcag aag gcg gag ggc ggc gtt ttg 624 Ile Asp Met Pro Met Glu Leu Leu GlnLys Ala Glu Gly Gly Val Leu 195 200 205 tat gtc ggc gac atc gcc cag tacagc cgc aac atc caa gcc ggt att 672 Tyr Val Gly Asp Ile Ala Gln Tyr SerArg Asn Ile Gln Ala Gly Ile 210 215 220 gcc ttt att gtc gga aag gcg gaacac cgc cgc gtc agg gtg gtc gca 720 Ala Phe Ile Val Gly Lys Ala Glu HisArg Arg Val Arg Val Val Ala 225 230 235 240 tcg ggc agc agg gcg gca ggttca gac ggc att gcc tgc gag gaa aag 768 Ser Gly Ser Arg Ala Ala Gly SerAsp Gly Ile Ala Cys Glu Glu Lys 245 250 255 ctg gcg gaa ctg ctg tcg gaatcg gtc gtc cgt att ccg ccg ctg cgt 816 Leu Ala Glu Leu Leu Ser Glu SerVal Val Arg Ile Pro Pro Leu Arg 260 265 270 atg cag cat gaa gac att cccttc ctg ata cag ggg att gcc tgc aat 864 Met Gln His Glu Asp Ile Pro PheLeu Ile Gln Gly Ile Ala Cys Asn 275 280 285 gtg gcg gaa agc caa aag attgcg cct gcc tca ttc agt gaa gag gca 912 Val Ala Glu Ser Gln Lys Ile AlaPro Ala Ser Phe Ser Glu Glu Ala 290 295 300 ctt gcc gca ttg acc cgt tacgac tgg ccg gga aat ttc gac caa ctg 960 Leu Ala Ala Leu Thr Arg Tyr AspTrp Pro Gly Asn Phe Asp Gln Leu 305 310 315 320 caa agc gtc gtt gca acgctg ttg ttg gag gcg gac gga cag gaa atc 1008 Gln Ser Val Val Ala Thr LeuLeu Leu Glu Ala Asp Gly Gln Glu Ile 325 330 335 ggc gca ggg gcg gtt tcttcc ctt ttg ggg cag aat gtg cct gcc gag 1056 Gly Ala Gly Ala Val Ser SerLeu Leu Gly Gln Asn Val Pro Ala Glu 340 345 350 ggg gcg gaa gat atg gtgggc ggg ttt aat ttc aac ctg ccc ctg cgc 1104 Gly Ala Glu Asp Met Val GlyGly Phe Asn Phe Asn Leu Pro Leu Arg 355 360 365 gaa ttg agg gag gag gtggag cgg cgt tat ttc gag tac cac atc gcc 1152 Glu Leu Arg Glu Glu Val GluArg Arg Tyr Phe Glu Tyr His Ile Ala 370 375 380 caa gaa ggt cag aat atgagc caa gtg gcg cag aaa gtt ggt ttg gaa 1200 Gln Glu Gly Gln Asn Met SerGln Val Ala Gln Lys Val Gly Leu Glu 385 390 395 400 cgc acg cac ctt taccgc aaa ctc aaa cag ctc ggc atc ggc gtt tcg 1248 Arg Thr His Leu Tyr ArgLys Leu Lys Gln Leu Gly Ile Gly Val Ser 405 410 415 cgc cgg gcg ggg gaaaaa acc gaa gaa 1275 Arg Arg Ala Gly Glu Lys Thr Glu Glu 420 425 42 425PRT Neisseria meningitidis 42 Met Arg Ser Ser Asp Ile Leu Ile Val AspAsp Glu Ile Gly Ile Arg 1 5 10 15 Asp Leu Leu Ser Glu Ile Leu Gln AspGlu Gly Tyr Ser Val Ala Leu 20 25 30 Ala Glu Asn Ala Glu Glu Ala Arg LysLeu Arg His Gln Ala Arg Pro 35 40 45 Ala Met Val Leu Leu Asp Ile Trp MetPro Asp Cys Asp Gly Ile Thr 50 55 60 Leu Leu Lys Glu Trp Ala Lys Asn GlyGln Leu Asn Met Pro Val Val 65 70 75 80 Met Met Ser Gly His Ala Ser IleAsp Thr Ala Val Glu Ala Thr Lys 85 90 95 Ile Gly Ala Ile Asp Phe Leu GluLys Pro Ile Ser Leu Gln Lys Leu 100 105 110 Leu Ser Ala Val Glu Asn AlaLeu Lys Tyr Gly Ala Ala Gln Thr Glu 115 120 125 Thr Gly Pro Val Phe AspLys Leu Gly Asn Ser Ala Ala Ile Gln Glu 130 135 140 Met Asn Arg Glu ValGly Ala Ala Val Lys Cys Ala Ser Pro Val Leu 145 150 155 160 Leu Thr GlyGlu Ala Gly Ser Pro Phe Glu Thr Val Ala Arg Tyr Phe 165 170 175 His LysAsn Gly Thr Pro Trp Val Ser Pro Ala Arg Val Glu Tyr Leu 180 185 190 IleAsp Met Pro Met Glu Leu Leu Gln Lys Ala Glu Gly Gly Val Leu 195 200 205Tyr Val Gly Asp Ile Ala Gln Tyr Ser Arg Asn Ile Gln Ala Gly Ile 210 215220 Ala Phe Ile Val Gly Lys Ala Glu His Arg Arg Val Arg Val Val Ala 225230 235 240 Ser Gly Ser Arg Ala Ala Gly Ser Asp Gly Ile Ala Cys Glu GluLys 245 250 255 Leu Ala Glu Leu Leu Ser Glu Ser Val Val Arg Ile Pro ProLeu Arg 260 265 270 Met Gln His Glu Asp Ile Pro Phe Leu Ile Gln Gly IleAla Cys Asn 275 280 285 Val Ala Glu Ser Gln Lys Ile Ala Pro Ala Ser PheSer Glu Glu Ala 290 295 300 Leu Ala Ala Leu Thr Arg Tyr Asp Trp Pro GlyAsn Phe Asp Gln Leu 305 310 315 320 Gln Ser Val Val Ala Thr Leu Leu LeuGlu Ala Asp Gly Gln Glu Ile 325 330 335 Gly Ala Gly Ala Val Ser Ser LeuLeu Gly Gln Asn Val Pro Ala Glu 340 345 350 Gly Ala Glu Asp Met Val GlyGly Phe Asn Phe Asn Leu Pro Leu Arg 355 360 365 Glu Leu Arg Glu Glu ValGlu Arg Arg Tyr Phe Glu Tyr His Ile Ala 370 375 380 Gln Glu Gly Gln AsnMet Ser Gln Val Ala Gln Lys Val Gly Leu Glu 385 390 395 400 Arg Thr HisLeu Tyr Arg Lys Leu Lys Gln Leu Gly Ile Gly Val Ser 405 410 415 Arg ArgAla Gly Glu Lys Thr Glu Glu 420 425 43 1191 DNA Neisseria meningitidisCDS (1)..(1191) NMB0116 43 atg aca gag gac gaa cgt ttc gcg tgg ctg caattg gcg ttt acg ccc 48 Met Thr Glu Asp Glu Arg Phe Ala Trp Leu Gln LeuAla Phe Thr Pro 1 5 10 15 tat atc ggc gcg gaa agt ttc ctg ctg ctg atgcgc cgt ttc ggc agc 96 Tyr Ile Gly Ala Glu Ser Phe Leu Leu Leu Met ArgArg Phe Gly Ser 20 25 30 gcg caa aat gcc ctg tcc gca ccg gcg gaa cag gtggcg gca ctg ata 144 Ala Gln Asn Ala Leu Ser Ala Pro Ala Glu Gln Val AlaAla Leu Ile 35 40 45 cgg cac aaa cag gcg ctt gag gct tgg cgc aat gcg gaaaaa cgc gct 192 Arg His Lys Gln Ala Leu Glu Ala Trp Arg Asn Ala Glu LysArg Ala 50 55 60 ctg gcg cgg cag gcg gca gaa gcg gca ttg gaa tgg gaa atgcgg gac 240 Leu Ala Arg Gln Ala Ala Glu Ala Ala Leu Glu Trp Glu Met ArgAsp 65 70 75 80 gga tgc cgc ctg atg ctg ctt cag gat gaa gat ttt ccc gaaatg ctg 288 Gly Cys Arg Leu Met Leu Leu Gln Asp Glu Asp Phe Pro Glu MetLeu 85 90 95 acg cag ggg ctg acc gcg cca ccg gtt ttg ttt ttg cgc ggc aacgtg 336 Thr Gln Gly Leu Thr Ala Pro Pro Val Leu Phe Leu Arg Gly Asn Val100 105 110 caa ctg ctg cac aaa cct tcc gcc gcc atc gtc ggc agc cgt catgcc 384 Gln Leu Leu His Lys Pro Ser Ala Ala Ile Val Gly Ser Arg His Ala115 120 125 acg ccg cag gcg atg cgg att gcc aaa gat ttc ggc aag tcg ttgggt 432 Thr Pro Gln Ala Met Arg Ile Ala Lys Asp Phe Gly Lys Ser Leu Gly130 135 140 ggg aaa ggc att ccc gtt gtg tcg ggt atg gct tcg ggc atc gatacc 480 Gly Lys Gly Ile Pro Val Val Ser Gly Met Ala Ser Gly Ile Asp Thr145 150 155 160 gcc gcc cat cag ggt gcg ttg cag gca gaa ggc ggc acc atcgcc gtg 528 Ala Ala His Gln Gly Ala Leu Gln Ala Glu Gly Gly Thr Ile AlaVal 165 170 175 tgg ggg acg ggc ata gac cgc att tat ccg ccg gtc aac aaaaac ctt 576 Trp Gly Thr Gly Ile Asp Arg Ile Tyr Pro Pro Val Asn Lys AsnLeu 180 185 190 gcc tat gaa atc gcc gaa aaa gga ttg att gtc agc gag ttcccc atc 624 Ala Tyr Glu Ile Ala Glu Lys Gly Leu Ile Val Ser Glu Phe ProIle 195 200 205 ggc acg cgg ccg tat gcc ggc aat ttt ccg cgc cgc aac cgcctg att 672 Gly Thr Arg Pro Tyr Ala Gly Asn Phe Pro Arg Arg Asn Arg LeuIle 210 215 220 gcc gcc ctg tcg caa gta acg ctg gtg gtt gaa gcc gcg ttggaa tcc 720 Ala Ala Leu Ser Gln Val Thr Leu Val Val Glu Ala Ala Leu GluSer 225 230 235 240 ggt tcg ctg att act gcc aga ttg gcg gcg gag atg gggcgc gaa gtg 768 Gly Ser Leu Ile Thr Ala Arg Leu Ala Ala Glu Met Gly ArgGlu Val 245 250 255 atg gcg gta ccc ggc tcg ata gac aat cca cac agt aaaggc tgc cac 816 Met Ala Val Pro Gly Ser Ile Asp Asn Pro His Ser Lys GlyCys His 260 265 270 aaa ctg att aaa gac ggc gca aaa ttg gtg gaa tgc ctggac gac atc 864 Lys Leu Ile Lys Asp Gly Ala Lys Leu Val Glu Cys Leu AspAsp Ile 275 280 285 ctg aac gaa tgc ccg ggg cta ttg caa aat acg ggt gcttca tca tat 912 Leu Asn Glu Cys Pro Gly Leu Leu Gln Asn Thr Gly Ala SerSer Tyr 290 295 300 tct ata aat aag gga ata cct gaa aag cgc atc act gccgtt cag acg 960 Ser Ile Asn Lys Gly Ile Pro Glu Lys Arg Ile Thr Ala ValGln Thr 305 310 315 320 gca tcc gac cag ctg tct ctg cct gaa ggc aaa atgccg tct gaa aag 1008 Ala Ser Asp Gln Leu Ser Leu Pro Glu Gly Lys Met ProSer Glu Lys 325 330 335 acg gag aac cga ccc gtc ggc ggc agt atc ttg gacagg atg ggt ttc 1056 Thr Glu Asn Arg Pro Val Gly Gly Ser Ile Leu Asp ArgMet Gly Phe 340 345 350 gac cca gtt cat ccc gac gtg ctt gcc gga cag ttggct atg cct gcc 1104 Asp Pro Val His Pro Asp Val Leu Ala Gly Gln Leu AlaMet Pro Ala 355 360 365 gca gat ttg tat gcc gca ctg ttg gaa ttg gaa ttggac ggc agc gtt 1152 Ala Asp Leu Tyr Ala Ala Leu Leu Glu Leu Glu Leu AspGly Ser Val 370 375 380 gcc gca atg ccc ggc ggc aga tac cag cgt atc cgaact 1191 Ala Ala Met Pro Gly Gly Arg Tyr Gln Arg Ile Arg Thr 385 390 39544 397 PRT Neisseria meningitidis 44 Met Thr Glu Asp Glu Arg Phe Ala TrpLeu Gln Leu Ala Phe Thr Pro 1 5 10 15 Tyr Ile Gly Ala Glu Ser Phe LeuLeu Leu Met Arg Arg Phe Gly Ser 20 25 30 Ala Gln Asn Ala Leu Ser Ala ProAla Glu Gln Val Ala Ala Leu Ile 35 40 45 Arg His Lys Gln Ala Leu Glu AlaTrp Arg Asn Ala Glu Lys Arg Ala 50 55 60 Leu Ala Arg Gln Ala Ala Glu AlaAla Leu Glu Trp Glu Met Arg Asp 65 70 75 80 Gly Cys Arg Leu Met Leu LeuGln Asp Glu Asp Phe Pro Glu Met Leu 85 90 95 Thr Gln Gly Leu Thr Ala ProPro Val Leu Phe Leu Arg Gly Asn Val 100 105 110 Gln Leu Leu His Lys ProSer Ala Ala Ile Val Gly Ser Arg His Ala 115 120 125 Thr Pro Gln Ala MetArg Ile Ala Lys Asp Phe Gly Lys Ser Leu Gly 130 135 140 Gly Lys Gly IlePro Val Val Ser Gly Met Ala Ser Gly Ile Asp Thr 145 150 155 160 Ala AlaHis Gln Gly Ala Leu Gln Ala Glu Gly Gly Thr Ile Ala Val 165 170 175 TrpGly Thr Gly Ile Asp Arg Ile Tyr Pro Pro Val Asn Lys Asn Leu 180 185 190Ala Tyr Glu Ile Ala Glu Lys Gly Leu Ile Val Ser Glu Phe Pro Ile 195 200205 Gly Thr Arg Pro Tyr Ala Gly Asn Phe Pro Arg Arg Asn Arg Leu Ile 210215 220 Ala Ala Leu Ser Gln Val Thr Leu Val Val Glu Ala Ala Leu Glu Ser225 230 235 240 Gly Ser Leu Ile Thr Ala Arg Leu Ala Ala Glu Met Gly ArgGlu Val 245 250 255 Met Ala Val Pro Gly Ser Ile Asp Asn Pro His Ser LysGly Cys His 260 265 270 Lys Leu Ile Lys Asp Gly Ala Lys Leu Val Glu CysLeu Asp Asp Ile 275 280 285 Leu Asn Glu Cys Pro Gly Leu Leu Gln Asn ThrGly Ala Ser Ser Tyr 290 295 300 Ser Ile Asn Lys Gly Ile Pro Glu Lys ArgIle Thr Ala Val Gln Thr 305 310 315 320 Ala Ser Asp Gln Leu Ser Leu ProGlu Gly Lys Met Pro Ser Glu Lys 325 330 335 Thr Glu Asn Arg Pro Val GlyGly Ser Ile Leu Asp Arg Met Gly Phe 340 345 350 Asp Pro Val His Pro AspVal Leu Ala Gly Gln Leu Ala Met Pro Ala 355 360 365 Ala Asp Leu Tyr AlaAla Leu Leu Glu Leu Glu Leu Asp Gly Ser Val 370 375 380 Ala Ala Met ProGly Gly Arg Tyr Gln Arg Ile Arg Thr 385 390 395 45 459 DNA Neisseriameningitidis CDS (1)..(459) NMB0117 45 atg acc gaa gtc atc gcc tac ctcatc gaa cat ttc caa gat ttc gat 48 Met Thr Glu Val Ile Ala Tyr Leu IleGlu His Phe Gln Asp Phe Asp 1 5 10 15 acc tgc ccg ccg ccc gaa gac ttgggt atg ctg ctt gaa gaa gcg ggt 96 Thr Cys Pro Pro Pro Glu Asp Leu GlyMet Leu Leu Glu Glu Ala Gly 20 25 30 ttc gat acg atg gaa atc ggc aac accctg atg atg atg gaa gta ttg 144 Phe Asp Thr Met Glu Ile Gly Asn Thr LeuMet Met Met Glu Val Leu 35 40 45 ctc aac agc tcc gaa ttt tcc gcc gaa cccgcc gac agc ggc gca ttg 192 Leu Asn Ser Ser Glu Phe Ser Ala Glu Pro AlaAsp Ser Gly Ala Leu 50 55 60 cgc gtg tac agc aaa gaa gaa acc gac aac ctgccg cag gaa gtg atg 240 Arg Val Tyr Ser Lys Glu Glu Thr Asp Asn Leu ProGln Glu Val Met 65 70 75 80 ggg ctg atg cag tat ctg att gaa gaa aaa gccgtc agc tgc gaa cag 288 Gly Leu Met Gln Tyr Leu Ile Glu Glu Lys Ala ValSer Cys Glu Gln 85 90 95 cgg gaa atc atc atc cac gcg ctc atg cac att ccgggc gac gaa att 336 Arg Glu Ile Ile Ile His Ala Leu Met His Ile Pro GlyAsp Glu Ile 100 105 110 acc gta gat acc gcc aaa gtg ctg acc ctg ctg ctttta tgg gca aac 384 Thr Val Asp Thr Ala Lys Val Leu Thr Leu Leu Leu LeuTrp Ala Asn 115 120 125 aag agc gag ctg ccc gtg ttg gtc ggc gac gag ctgatg agc gcg ctt 432 Lys Ser Glu Leu Pro Val Leu Val Gly Asp Glu Leu MetSer Ala Leu 130 135 140 tta ctc gac aac aaa ccc acg atg aac 459 Leu LeuAsp Asn Lys Pro Thr Met Asn 145 150 46 153 PRT Neisseria meningitidis 46Met Thr Glu Val Ile Ala Tyr Leu Ile Glu His Phe Gln Asp Phe Asp 1 5 1015 Thr Cys Pro Pro Pro Glu Asp Leu Gly Met Leu Leu Glu Glu Ala Gly 20 2530 Phe Asp Thr Met Glu Ile Gly Asn Thr Leu Met Met Met Glu Val Leu 35 4045 Leu Asn Ser Ser Glu Phe Ser Ala Glu Pro Ala Asp Ser Gly Ala Leu 50 5560 Arg Val Tyr Ser Lys Glu Glu Thr Asp Asn Leu Pro Gln Glu Val Met 65 7075 80 Gly Leu Met Gln Tyr Leu Ile Glu Glu Lys Ala Val Ser Cys Glu Gln 8590 95 Arg Glu Ile Ile Ile His Ala Leu Met His Ile Pro Gly Asp Glu Ile100 105 110 Thr Val Asp Thr Ala Lys Val Leu Thr Leu Leu Leu Leu Trp AlaAsn 115 120 125 Lys Ser Glu Leu Pro Val Leu Val Gly Asp Glu Leu Met SerAla Leu 130 135 140 Leu Leu Asp Asn Lys Pro Thr Met Asn 145 150 47 1776DNA Neisseria meningitidis CDS (1)..(1776) NMB2075 47 atg acg gtt ttgaag ctt tcg cac tgg cgg gtg ttg gcg gag ctt gcc 48 Met Thr Val Leu LysLeu Ser His Trp Arg Val Leu Ala Glu Leu Ala 1 5 10 15 gac ggt ttg ccgcaa cac gtc tcg caa ctg gcg cgt atg gcg gat atg 96 Asp Gly Leu Pro GlnHis Val Ser Gln Leu Ala Arg Met Ala Asp Met 20 25 30 aag ccg cag cag ctcaac ggt ttt tgg cag cag atg ccg gcg cac ata 144 Lys Pro Gln Gln Leu AsnGly Phe Trp Gln Gln Met Pro Ala His Ile 35 40 45 cgc ggg ctg ttg cgc caacac gac ggc tat tgg cgg ctg gtg cgc cca 192 Arg Gly Leu Leu Arg Gln HisAsp Gly Tyr Trp Arg Leu Val Arg Pro 50 55 60 ttg gcg gtt ttc gat gcc gaaggt ttg cgc gag ctg ggg gaa agg tcg 240 Leu Ala Val Phe Asp Ala Glu GlyLeu Arg Glu Leu Gly Glu Arg Ser 65 70 75 80 ggt ttt cag acg gca ttg aagcac gag tgc gcg tcc agc aac gac gag 288 Gly Phe Gln Thr Ala Leu Lys HisGlu Cys Ala Ser Ser Asn Asp Glu 85 90 95 ata ctg gaa ttg gcg cgg att gcgccg gac aag gcg cac aaa acc ata 336 Ile Leu Glu Leu Ala Arg Ile Ala ProAsp Lys Ala His Lys Thr Ile 100 105 110 tgc gtg acc cac ctg caa agt aagggc agg ggg cgg cag ggg cgg aag 384 Cys Val Thr His Leu Gln Ser Lys GlyArg Gly Arg Gln Gly Arg Lys 115 120 125 tgg tcg cac cgt ttg ggc gag tgtctg atg ttc agt ttt ggc tgg gtg 432 Trp Ser His Arg Leu Gly Glu Cys LeuMet Phe Ser Phe Gly Trp Val 130 135 140 ttt gac cgg ccg cag tat gag ttgggt tcg ctg tcg cct gtt gcg gca 480 Phe Asp Arg Pro Gln Tyr Glu Leu GlySer Leu Ser Pro Val Ala Ala 145 150 155 160 gtg gcg tgt cgg cgc gcc ttgtcg cgt tta ggt ttg gat gtg cag att 528 Val Ala Cys Arg Arg Ala Leu SerArg Leu Gly Leu Asp Val Gln Ile 165 170 175 aag tgg ccc aat gat ttg gttgtc gga cgc gac aaa ttg ggc ggc att 576 Lys Trp Pro Asn Asp Leu Val ValGly Arg Asp Lys Leu Gly Gly Ile 180 185 190 ctg att gaa acg gtc agg acgggc ggc aaa acg gtt gcc gtg gtc ggt 624 Leu Ile Glu Thr Val Arg Thr GlyGly Lys Thr Val Ala Val Val Gly 195 200 205 atc ggc atc aat ttt gtc ctgccc aag gaa gta gaa aat gcc gct tcc 672 Ile Gly Ile Asn Phe Val Leu ProLys Glu Val Glu Asn Ala Ala Ser 210 215 220 gtg caa tcg ctg ttt cag acggca tcg cgg cgg ggc aat gcc gat gcc 720 Val Gln Ser Leu Phe Gln Thr AlaSer Arg Arg Gly Asn Ala Asp Ala 225 230 235 240 gcc gtg ctg ctg gaa acgctg ttg gtg gaa ctg gac gcg gtg ttg ttg 768 Ala Val Leu Leu Glu Thr LeuLeu Val Glu Leu Asp Ala Val Leu Leu 245 250 255 caa tat gcg cgg gac ggattt gcg cct ttt gtg gcg gaa tat cag gct 816 Gln Tyr Ala Arg Asp Gly PheAla Pro Phe Val Ala Glu Tyr Gln Ala 260 265 270 gcc aac cgc gac cac ggcaag gcg gta ttg ctg ttg cgc gac ggc gaa 864 Ala Asn Arg Asp His Gly LysAla Val Leu Leu Leu Arg Asp Gly Glu 275 280 285 acc gtg ttc gaa ggc acggtt aaa ggc gtg gac gga caa ggc gtt ttg 912 Thr Val Phe Glu Gly Thr ValLys Gly Val Asp Gly Gln Gly Val Leu 290 295 300 cac ttg gaa acg gca gagggc aaa cag acg gtc gtc agc ggc gaa atc 960 His Leu Glu Thr Ala Glu GlyLys Gln Thr Val Val Ser Gly Glu Ile 305 310 315 320 agc ctg cgg tcc gacgac agg ccg gtt tcc gtg ccg aag cgg cgg gat 1008 Ser Leu Arg Ser Asp AspArg Pro Val Ser Val Pro Lys Arg Arg Asp 325 330 335 tcg gaa cgt ttt ctgctg ttg gac ggc ggc aac agc cgg ctc aag tgg 1056 Ser Glu Arg Phe Leu LeuLeu Asp Gly Gly Asn Ser Arg Leu Lys Trp 340 345 350 gcg tgg gtg gaa aacggc acg ttc gca acc gtc ggt agc gcg ccg tac 1104 Ala Trp Val Glu Asn GlyThr Phe Ala Thr Val Gly Ser Ala Pro Tyr 355 360 365 cgc gat ttg tcg cctttg ggc gcg gag tgg gcg gaa aag gcg gat gga 1152 Arg Asp Leu Ser Pro LeuGly Ala Glu Trp Ala Glu Lys Ala Asp Gly 370 375 380 aat gtc cgc atc gtcggt tgc gct gtg tgc gga gaa ttc aaa aag gca 1200 Asn Val Arg Ile Val GlyCys Ala Val Cys Gly Glu Phe Lys Lys Ala 385 390 395 400 caa gtg cag gaacag ctc gcc cga aaa atc gag tgg ctg ccg tct tcc 1248 Gln Val Gln Glu GlnLeu Ala Arg Lys Ile Glu Trp Leu Pro Ser Ser 405 410 415 gca cag gct ttgggc ata cgc aac cac tac cgc cac ccc gaa gaa cac 1296 Ala Gln Ala Leu GlyIle Arg Asn His Tyr Arg His Pro Glu Glu His 420 425 430 ggt tcc gac cgctgg ttc aac gcc ttg ggc agc cgc cgc ttc agc cgc 1344 Gly Ser Asp Arg TrpPhe Asn Ala Leu Gly Ser Arg Arg Phe Ser Arg 435 440 445 aac gcc tgc gtcgtc gtc agt tgc ggc acg gcg gta acg gtt gac gcg 1392 Asn Ala Cys Val ValVal Ser Cys Gly Thr Ala Val Thr Val Asp Ala 450 455 460 ctc acc gat gacgga cat tat ctc ggg gga acc atc atg ccc ggt ttc 1440 Leu Thr Asp Asp GlyHis Tyr Leu Gly Gly Thr Ile Met Pro Gly Phe 465 470 475 480 cac ctg atgaaa gaa tcg ctc gcc gtc cga acc gcc aac ctc aac cgg 1488 His Leu Met LysGlu Ser Leu Ala Val Arg Thr Ala Asn Leu Asn Arg 485 490 495 cac gcc ggtaag cgt tat cct ttc ccg acc aca acg ggc aat gcc gtc 1536 His Ala Gly LysArg Tyr Pro Phe Pro Thr Thr Thr Gly Asn Ala Val 500 505 510 gcc agc ggcatg atg gat gcg gtt tgc ggc tcg gtt atg atg atg cac 1584 Ala Ser Gly MetMet Asp Ala Val Cys Gly Ser Val Met Met Met His 515 520 525 ggg cgt ttgaaa gaa aaa acc ggg gcg ggc aag cct gtc gat gtc atc 1632 Gly Arg Leu LysGlu Lys Thr Gly Ala Gly Lys Pro Val Asp Val Ile 530 535 540 att acc ggcggc ggc gcg gca aaa gtt gcc gaa gcc ctg ccg cct gca 1680 Ile Thr Gly GlyGly Ala Ala Lys Val Ala Glu Ala Leu Pro Pro Ala 545 550 555 560 ttt ttggcg gaa aat acc gtg cgc gtg gcg gac aac ctc gtc att tac 1728 Phe Leu AlaGlu Asn Thr Val Arg Val Ala Asp Asn Leu Val Ile Tyr 565 570 575 ggg ttgttg aac atg att gcc gcc gaa ggc agg gaa tat gaa cat att 1776 Gly Leu LeuAsn Met Ile Ala Ala Glu Gly Arg Glu Tyr Glu His Ile 580 585 590 48 592PRT Neisseria meningitidis 48 Met Thr Val Leu Lys Leu Ser His Trp ArgVal Leu Ala Glu Leu Ala 1 5 10 15 Asp Gly Leu Pro Gln His Val Ser GlnLeu Ala Arg Met Ala Asp Met 20 25 30 Lys Pro Gln Gln Leu Asn Gly Phe TrpGln Gln Met Pro Ala His Ile 35 40 45 Arg Gly Leu Leu Arg Gln His Asp GlyTyr Trp Arg Leu Val Arg Pro 50 55 60 Leu Ala Val Phe Asp Ala Glu Gly LeuArg Glu Leu Gly Glu Arg Ser 65 70 75 80 Gly Phe Gln Thr Ala Leu Lys HisGlu Cys Ala Ser Ser Asn Asp Glu 85 90 95 Ile Leu Glu Leu Ala Arg Ile AlaPro Asp Lys Ala His Lys Thr Ile 100 105 110 Cys Val Thr His Leu Gln SerLys Gly Arg Gly Arg Gln Gly Arg Lys 115 120 125 Trp Ser His Arg Leu GlyGlu Cys Leu Met Phe Ser Phe Gly Trp Val 130 135 140 Phe Asp Arg Pro GlnTyr Glu Leu Gly Ser Leu Ser Pro Val Ala Ala 145 150 155 160 Val Ala CysArg Arg Ala Leu Ser Arg Leu Gly Leu Asp Val Gln Ile 165 170 175 Lys TrpPro Asn Asp Leu Val Val Gly Arg Asp Lys Leu Gly Gly Ile 180 185 190 LeuIle Glu Thr Val Arg Thr Gly Gly Lys Thr Val Ala Val Val Gly 195 200 205Ile Gly Ile Asn Phe Val Leu Pro Lys Glu Val Glu Asn Ala Ala Ser 210 215220 Val Gln Ser Leu Phe Gln Thr Ala Ser Arg Arg Gly Asn Ala Asp Ala 225230 235 240 Ala Val Leu Leu Glu Thr Leu Leu Val Glu Leu Asp Ala Val LeuLeu 245 250 255 Gln Tyr Ala Arg Asp Gly Phe Ala Pro Phe Val Ala Glu TyrGln Ala 260 265 270 Ala Asn Arg Asp His Gly Lys Ala Val Leu Leu Leu ArgAsp Gly Glu 275 280 285 Thr Val Phe Glu Gly Thr Val Lys Gly Val Asp GlyGln Gly Val Leu 290 295 300 His Leu Glu Thr Ala Glu Gly Lys Gln Thr ValVal Ser Gly Glu Ile 305 310 315 320 Ser Leu Arg Ser Asp Asp Arg Pro ValSer Val Pro Lys Arg Arg Asp 325 330 335 Ser Glu Arg Phe Leu Leu Leu AspGly Gly Asn Ser Arg Leu Lys Trp 340 345 350 Ala Trp Val Glu Asn Gly ThrPhe Ala Thr Val Gly Ser Ala Pro Tyr 355 360 365 Arg Asp Leu Ser Pro LeuGly Ala Glu Trp Ala Glu Lys Ala Asp Gly 370 375 380 Asn Val Arg Ile ValGly Cys Ala Val Cys Gly Glu Phe Lys Lys Ala 385 390 395 400 Gln Val GlnGlu Gln Leu Ala Arg Lys Ile Glu Trp Leu Pro Ser Ser 405 410 415 Ala GlnAla Leu Gly Ile Arg Asn His Tyr Arg His Pro Glu Glu His 420 425 430 GlySer Asp Arg Trp Phe Asn Ala Leu Gly Ser Arg Arg Phe Ser Arg 435 440 445Asn Ala Cys Val Val Val Ser Cys Gly Thr Ala Val Thr Val Asp Ala 450 455460 Leu Thr Asp Asp Gly His Tyr Leu Gly Gly Thr Ile Met Pro Gly Phe 465470 475 480 His Leu Met Lys Glu Ser Leu Ala Val Arg Thr Ala Asn Leu AsnArg 485 490 495 His Ala Gly Lys Arg Tyr Pro Phe Pro Thr Thr Thr Gly AsnAla Val 500 505 510 Ala Ser Gly Met Met Asp Ala Val Cys Gly Ser Val MetMet Met His 515 520 525 Gly Arg Leu Lys Glu Lys Thr Gly Ala Gly Lys ProVal Asp Val Ile 530 535 540 Ile Thr Gly Gly Gly Ala Ala Lys Val Ala GluAla Leu Pro Pro Ala 545 550 555 560 Phe Leu Ala Glu Asn Thr Val Arg ValAla Asp Asn Leu Val Ile Tyr 565 570 575 Gly Leu Leu Asn Met Ile Ala AlaGlu Gly Arg Glu Tyr Glu His Ile 580 585 590 49 864 DNA Neisseriameningitidis CDS (1)..(864) NMB2074 49 atg aaa tgg cta ttt atc ctt ttggtt gcg att aat att gcc gta ttc 48 Met Lys Trp Leu Phe Ile Leu Leu ValAla Ile Asn Ile Ala Val Phe 1 5 10 15 ggc ggt acg gta ggt tac aaa ctgaca ctg aaa cag gcc ggc aga ata 96 Gly Gly Thr Val Gly Tyr Lys Leu ThrLeu Lys Gln Ala Gly Arg Ile 20 25 30 ccg gag gca cag aat gcc gca aac aatttg cag gtt caa cca gtt gcc 144 Pro Glu Ala Gln Asn Ala Ala Asn Asn LeuGln Val Gln Pro Val Ala 35 40 45 cca act atg ccg gtt gtt cgg aat att ccagca tcc ggt cct gtc gtt 192 Pro Thr Met Pro Val Val Arg Asn Ile Pro AlaSer Gly Pro Val Val 50 55 60 cag gcg gca tct gaa tcg gat aca ggc gca ctgctc aaa cag ggc gac 240 Gln Ala Ala Ser Glu Ser Asp Thr Gly Ala Leu LeuLys Gln Gly Asp 65 70 75 80 att ctg agc gaa gaa cag gcg gag cag ttg cgcttg aaa aaa gaa gcg 288 Ile Leu Ser Glu Glu Gln Ala Glu Gln Leu Arg LeuLys Lys Glu Ala 85 90 95 gaa cag aaa aaa ctg aaa gag aaa aaa cag cgt gaagaa aaa gcc cgc 336 Glu Gln Lys Lys Leu Lys Glu Lys Lys Gln Arg Glu GluLys Ala Arg 100 105 110 cgc gaa aaa ctc gcc gcc gaa aag gcg cag gcg gaacgc gaa aac ggc 384 Arg Glu Lys Leu Ala Ala Glu Lys Ala Gln Ala Glu ArgGlu Asn Gly 115 120 125 gcg gcg gat gcc tta tgc gcc gcg cag gca agc ctcacg atg gac gaa 432 Ala Ala Asp Ala Leu Cys Ala Ala Gln Ala Ser Leu ThrMet Asp Glu 130 135 140 gat gac tac cac cgc atc aaa gga ctt ttg ggc aaatgg tcg cac gtt 480 Asp Asp Tyr His Arg Ile Lys Gly Leu Leu Gly Lys TrpSer His Val 145 150 155 160 gcc agc agg agc gtc gaa aaa cgc acc gcc caagcc aaa cct gcc gac 528 Ala Ser Arg Ser Val Glu Lys Arg Thr Ala Gln AlaLys Pro Ala Asp 165 170 175 aaa acc tac cgc gtc gtc ctg ccc gtt tcc gccgat gcc gaa aat cag 576 Lys Thr Tyr Arg Val Val Leu Pro Val Ser Ala AspAla Glu Asn Gln 180 185 190 gcg gcg gag ctg tct gcc aaa ggt ttc aac cccata ccg ttt gac ggc 624 Ala Ala Glu Leu Ser Ala Lys Gly Phe Asn Pro IlePro Phe Asp Gly 195 200 205 gca ttg agt ttg ggt gtc ggc aac agc cgg gaaaac gcc caa gcc ctg 672 Ala Leu Ser Leu Gly Val Gly Asn Ser Arg Glu AsnAla Gln Ala Leu 210 215 220 caa aac cgg ctt gcc ggc gcc gga ttc ggc ggggcg cat att gtc gaa 720 Gln Asn Arg Leu Ala Gly Ala Gly Phe Gly Gly AlaHis Ile Val Glu 225 230 235 240 cac ttt gcc gaa gcc gac agg cag gac gattct ttg agc gtg tcg cgt 768 His Phe Ala Glu Ala Asp Arg Gln Asp Asp SerLeu Ser Val Ser Arg 245 250 255 atg acg gtt ttg ttt acc ggc gtg aat gccgcc gat gcg gac gaa att 816 Met Thr Val Leu Phe Thr Gly Val Asn Ala AlaAsp Ala Asp Glu Ile 260 265 270 cgt aaa atc acg tcc cta tac ggc aaa ctgaac ctc aag tct tgc aaa 864 Arg Lys Ile Thr Ser Leu Tyr Gly Lys Leu AsnLeu Lys Ser Cys Lys 275 280 285 50 288 PRT Neisseria meningitidis 50 MetLys Trp Leu Phe Ile Leu Leu Val Ala Ile Asn Ile Ala Val Phe 1 5 10 15Gly Gly Thr Val Gly Tyr Lys Leu Thr Leu Lys Gln Ala Gly Arg Ile 20 25 30Pro Glu Ala Gln Asn Ala Ala Asn Asn Leu Gln Val Gln Pro Val Ala 35 40 45Pro Thr Met Pro Val Val Arg Asn Ile Pro Ala Ser Gly Pro Val Val 50 55 60Gln Ala Ala Ser Glu Ser Asp Thr Gly Ala Leu Leu Lys Gln Gly Asp 65 70 7580 Ile Leu Ser Glu Glu Gln Ala Glu Gln Leu Arg Leu Lys Lys Glu Ala 85 9095 Glu Gln Lys Lys Leu Lys Glu Lys Lys Gln Arg Glu Glu Lys Ala Arg 100105 110 Arg Glu Lys Leu Ala Ala Glu Lys Ala Gln Ala Glu Arg Glu Asn Gly115 120 125 Ala Ala Asp Ala Leu Cys Ala Ala Gln Ala Ser Leu Thr Met AspGlu 130 135 140 Asp Asp Tyr His Arg Ile Lys Gly Leu Leu Gly Lys Trp SerHis Val 145 150 155 160 Ala Ser Arg Ser Val Glu Lys Arg Thr Ala Gln AlaLys Pro Ala Asp 165 170 175 Lys Thr Tyr Arg Val Val Leu Pro Val Ser AlaAsp Ala Glu Asn Gln 180 185 190 Ala Ala Glu Leu Ser Ala Lys Gly Phe AsnPro Ile Pro Phe Asp Gly 195 200 205 Ala Leu Ser Leu Gly Val Gly Asn SerArg Glu Asn Ala Gln Ala Leu 210 215 220 Gln Asn Arg Leu Ala Gly Ala GlyPhe Gly Gly Ala His Ile Val Glu 225 230 235 240 His Phe Ala Glu Ala AspArg Gln Asp Asp Ser Leu Ser Val Ser Arg 245 250 255 Met Thr Val Leu PheThr Gly Val Asn Ala Ala Asp Ala Asp Glu Ile 260 265 270 Arg Lys Ile ThrSer Leu Tyr Gly Lys Leu Asn Leu Lys Ser Cys Lys 275 280 285 51 786 DNANeisseria meningitidis CDS (1)..(786) NMA 0385 51 atg aaa caa gct atgaaa aat tgg ttt gct gcc ttg ctg ttg gca gta 48 Met Lys Gln Ala Met LysAsn Trp Phe Ala Ala Leu Leu Leu Ala Val 1 5 10 15 ccc atg ggt gcg gcattt gct tcg ggc ggt cat gca cac tat gag aaa 96 Pro Met Gly Ala Ala PheAla Ser Gly Gly His Ala His Tyr Glu Lys 20 25 30 gtc gat atc gat ttg cgcgac caa gtc agc ctg cag cgc ggc gca caa 144 Val Asp Ile Asp Leu Arg AspGln Val Ser Leu Gln Arg Gly Ala Gln 35 40 45 atc ttt acc aac tat tgc ctgtcc tgc cac tcg gca agc ggt atg cgt 192 Ile Phe Thr Asn Tyr Cys Leu SerCys His Ser Ala Ser Gly Met Arg 50 55 60 ttc aac cgt ttg aaa gac atc ggtttg act gac gaa gaa atc aag aaa 240 Phe Asn Arg Leu Lys Asp Ile Gly LeuThr Asp Glu Glu Ile Lys Lys 65 70 75 80 aac ctg atg ttt acc acc gat aatgtc ggc gat gtc atg cat tcg gcg 288 Asn Leu Met Phe Thr Thr Asp Asn ValGly Asp Val Met His Ser Ala 85 90 95 atg aac ccg aaa gat gcg gca aaa tggttt ggt gct gct ccg ccc gat 336 Met Asn Pro Lys Asp Ala Ala Lys Trp PheGly Ala Ala Pro Pro Asp 100 105 110 ttg aca ttg att gcg cgt tcc aaa ggtgca gac tac ctt tac gct tat 384 Leu Thr Leu Ile Ala Arg Ser Lys Gly AlaAsp Tyr Leu Tyr Ala Tyr 115 120 125 atg cgc ggc ttc tat aaa gat ccg acccgt ccg agc ggc tgg aac aat 432 Met Arg Gly Phe Tyr Lys Asp Pro Thr ArgPro Ser Gly Trp Asn Asn 130 135 140 act gta ttc gat aaa gtc ggt atg ccccac ccg ttg tgg gaa cag caa 480 Thr Val Phe Asp Lys Val Gly Met Pro HisPro Leu Trp Glu Gln Gln 145 150 155 160 ggc gtt caa gcc gtt gag ttg gatgcc aaa ggt cag ccg gtt atg gta 528 Gly Val Gln Ala Val Glu Leu Asp AlaLys Gly Gln Pro Val Met Val 165 170 175 aaa gac gaa cac ggc gag atg aagcct aag ctg tat tgg gaa tct acc 576 Lys Asp Glu His Gly Glu Met Lys ProLys Leu Tyr Trp Glu Ser Thr 180 185 190 ggt ttg cac agc cgc cgt ctg cctaac ggc aaa gtg atc caa aaa gag 624 Gly Leu His Ser Arg Arg Leu Pro AsnGly Lys Val Ile Gln Lys Glu 195 200 205 tac gac gca tat gta cgc gat ttggtc aat tac ctt gtg tac atg ggc 672 Tyr Asp Ala Tyr Val Arg Asp Leu ValAsn Tyr Leu Val Tyr Met Gly 210 215 220 gaa cct gca caa ctg caa cgc aaacgt ata ggc tat gtc gtg atg att 720 Glu Pro Ala Gln Leu Gln Arg Lys ArgIle Gly Tyr Val Val Met Ile 225 230 235 240 ttc cta ttt gcg gtt atg ctgcct ttg gcg tat ttc ctg aaa aaa gaa 768 Phe Leu Phe Ala Val Met Leu ProLeu Ala Tyr Phe Leu Lys Lys Glu 245 250 255 tat tgg aaa gac gta cac 786Tyr Trp Lys Asp Val His 260 52 262 PRT Neisseria meningitidis 52 Met LysGln Ala Met Lys Asn Trp Phe Ala Ala Leu Leu Leu Ala Val 1 5 10 15 ProMet Gly Ala Ala Phe Ala Ser Gly Gly His Ala His Tyr Glu Lys 20 25 30 ValAsp Ile Asp Leu Arg Asp Gln Val Ser Leu Gln Arg Gly Ala Gln 35 40 45 IlePhe Thr Asn Tyr Cys Leu Ser Cys His Ser Ala Ser Gly Met Arg 50 55 60 PheAsn Arg Leu Lys Asp Ile Gly Leu Thr Asp Glu Glu Ile Lys Lys 65 70 75 80Asn Leu Met Phe Thr Thr Asp Asn Val Gly Asp Val Met His Ser Ala 85 90 95Met Asn Pro Lys Asp Ala Ala Lys Trp Phe Gly Ala Ala Pro Pro Asp 100 105110 Leu Thr Leu Ile Ala Arg Ser Lys Gly Ala Asp Tyr Leu Tyr Ala Tyr 115120 125 Met Arg Gly Phe Tyr Lys Asp Pro Thr Arg Pro Ser Gly Trp Asn Asn130 135 140 Thr Val Phe Asp Lys Val Gly Met Pro His Pro Leu Trp Glu GlnGln 145 150 155 160 Gly Val Gln Ala Val Glu Leu Asp Ala Lys Gly Gln ProVal Met Val 165 170 175 Lys Asp Glu His Gly Glu Met Lys Pro Lys Leu TyrTrp Glu Ser Thr 180 185 190 Gly Leu His Ser Arg Arg Leu Pro Asn Gly LysVal Ile Gln Lys Glu 195 200 205 Tyr Asp Ala Tyr Val Arg Asp Leu Val AsnTyr Leu Val Tyr Met Gly 210 215 220 Glu Pro Ala Gln Leu Gln Arg Lys ArgIle Gly Tyr Val Val Met Ile 225 230 235 240 Phe Leu Phe Ala Val Met LeuPro Leu Ala Tyr Phe Leu Lys Lys Glu 245 250 255 Tyr Trp Lys Asp Val His260 53 1782 DNA Neisseria meningitidis CDS (1)..(1782) NMB2050 53 atggaa ctg atg act gtt ttg ctg cct ttg gcg gcg ttg gtg tcg ggc 48 Met GluLeu Met Thr Val Leu Leu Pro Leu Ala Ala Leu Val Ser Gly 1 5 10 15 gtgttg ttt aca tgg ttg ctg atg aag ggc cgg ttt cag ggc gag ttt 96 Val LeuPhe Thr Trp Leu Leu Met Lys Gly Arg Phe Gln Gly Glu Phe 20 25 30 gcc ggtttg aac gcg cac ctg gcg gaa aag gcg gca aga tgt gat ttt 144 Ala Gly LeuAsn Ala His Leu Ala Glu Lys Ala Ala Arg Cys Asp Phe 35 40 45 gtc gaa caggca cac ggc aaa acc gtg tcg gaa ttg gcg gtg ttg gac 192 Val Glu Gln AlaHis Gly Lys Thr Val Ser Glu Leu Ala Val Leu Asp 50 55 60 ggg aaa tac cggcat ttg cag gac gaa aat tat gct ttg ggc aac cgt 240 Gly Lys Tyr Arg HisLeu Gln Asp Glu Asn Tyr Ala Leu Gly Asn Arg 65 70 75 80 ttt tcc gca gccgaa aag cag att gcc cat ttg cag gaa aaa gag gcg 288 Phe Ser Ala Ala GluLys Gln Ile Ala His Leu Gln Glu Lys Glu Ala 85 90 95 gag tcg gcg cgg ctgaag cag tcg tat atc gag ttg cag gaa aag gca 336 Glu Ser Ala Arg Leu LysGln Ser Tyr Ile Glu Leu Gln Glu Lys Ala 100 105 110 cag ggt ttg gcg gttgaa aac gaa cgt ttg gca acg cag ctc gga cag 384 Gln Gly Leu Ala Val GluAsn Glu Arg Leu Ala Thr Gln Leu Gly Gln 115 120 125 gaa cgg aag gcg tttgcc gac caa tat gcc ttg gaa cgc caa atc cgc 432 Glu Arg Lys Ala Phe AlaAsp Gln Tyr Ala Leu Glu Arg Gln Ile Arg 130 135 140 caa aga atc gaa accgat ttg gaa gaa agc cgc caa act gtc cgc gac 480 Gln Arg Ile Glu Thr AspLeu Glu Glu Ser Arg Gln Thr Val Arg Asp 145 150 155 160 gtg caa aac gacctt tcc gat gtc ggc aac cgt ttt gcc gca gcc gaa 528 Val Gln Asn Asp LeuSer Asp Val Gly Asn Arg Phe Ala Ala Ala Glu 165 170 175 aaa cag att gcccat ttg cag gaa aaa gag gcg gaa gcg gag cgg ttg 576 Lys Gln Ile Ala HisLeu Gln Glu Lys Glu Ala Glu Ala Glu Arg Leu 180 185 190 agg cag tcg catacc gag ttg cag gaa aag gca cag ggt ttg gcg gtt 624 Arg Gln Ser His ThrGlu Leu Gln Glu Lys Ala Gln Gly Leu Ala Val 195 200 205 gaa aac gaa cgtttg gca acg caa atc gaa cag gaa cgc ctt gct tct 672 Glu Asn Glu Arg LeuAla Thr Gln Ile Glu Gln Glu Arg Leu Ala Ser 210 215 220 gaa gag aag ctgtcc ttg ctg ggc gag gcg cgc aaa agt ttg agc gat 720 Glu Glu Lys Leu SerLeu Leu Gly Glu Ala Arg Lys Ser Leu Ser Asp 225 230 235 240 cag ttt caaaat ctt gcc aac acg att ttg gaa gaa aaa agc cgc cgt 768 Gln Phe Gln AsnLeu Ala Asn Thr Ile Leu Glu Glu Lys Ser Arg Arg 245 250 255 ttt acc gagcag aac cgc gag cag ctc cat cag gtt ttg aac ccg cta 816 Phe Thr Glu GlnAsn Arg Glu Gln Leu His Gln Val Leu Asn Pro Leu 260 265 270 aac gaa cgcatc cac ggt ttc ggc gag ttg gtc aag caa acc tat gat 864 Asn Glu Arg IleHis Gly Phe Gly Glu Leu Val Lys Gln Thr Tyr Asp 275 280 285 aaa gaa tcgcgc gag cgg ctg acg ttg gaa aac gaa ttg aaa cgg ctt 912 Lys Glu Ser ArgGlu Arg Leu Thr Leu Glu Asn Glu Leu Lys Arg Leu 290 295 300 cag ggg ttgaac gcg cag ctg cac agc gag gca aag gcc ctg acc aac 960 Gln Gly Leu AsnAla Gln Leu His Ser Glu Ala Lys Ala Leu Thr Asn 305 310 315 320 gcg ctgacc ggt acg cag aat aag gtt cag ggc aat tgg ggc gag atg 1008 Ala Leu ThrGly Thr Gln Asn Lys Val Gln Gly Asn Trp Gly Glu Met 325 330 335 att ctggaa acg gtt ttg gaa aat tcc ggc ctt cag aaa ggg cgg gaa 1056 Ile Leu GluThr Val Leu Glu Asn Ser Gly Leu Gln Lys Gly Arg Glu 340 345 350 tat gtggtt cag gcg gca tcc gtc cga aaa gag gaa gac ggc ggc acg 1104 Tyr Val ValGln Ala Ala Ser Val Arg Lys Glu Glu Asp Gly Gly Thr 355 360 365 cgc cgcctc cag ccc gac gtt ttg gtc aac ctg ccc gac aac aag cag 1152 Arg Arg LeuGln Pro Asp Val Leu Val Asn Leu Pro Asp Asn Lys Gln 370 375 380 att gtgatt gat tcc aag gtc tcg ctg aca gct tat gtg cgc tac acg 1200 Ile Val IleAsp Ser Lys Val Ser Leu Thr Ala Tyr Val Arg Tyr Thr 385 390 395 400 caggcg gcg gat gcg gat acg gcg gca cgc gaa ctg gcg gca cac gtt 1248 Gln AlaAla Asp Ala Asp Thr Ala Ala Arg Glu Leu Ala Ala His Val 405 410 415 gccagc atc cgt gca cac atg aaa ggc ttg tcg ctg aag gat tac acc 1296 Ala SerIle Arg Ala His Met Lys Gly Leu Ser Leu Lys Asp Tyr Thr 420 425 430 gatttg gaa ggt gtg aac aca ttg gat ttc gtc ttt atg ttt atc cct 1344 Asp LeuGlu Gly Val Asn Thr Leu Asp Phe Val Phe Met Phe Ile Pro 435 440 445 gtcgaa ccg gcc tac ctg ttg gcg ttg cag aat gac gcg ggc ttg ttc 1392 Val GluPro Ala Tyr Leu Leu Ala Leu Gln Asn Asp Ala Gly Leu Phe 450 455 460 caagag tgt ttc gac aaa cgg att atg ctg gtc ggc ccc agt acg ctg 1440 Gln GluCys Phe Asp Lys Arg Ile Met Leu Val Gly Pro Ser Thr Leu 465 470 475 480ctg gcg act ttg agg acg gtg gcg aat att tgg cgc aac gaa cag caa 1488 LeuAla Thr Leu Arg Thr Val Ala Asn Ile Trp Arg Asn Glu Gln Gln 485 490 495aat cag aac gca ctg gcg att gcg gac gaa ggc ggc aag ctg tac gac 1536 AsnGln Asn Ala Leu Ala Ile Ala Asp Glu Gly Gly Lys Leu Tyr Asp 500 505 510aag ttt gtc ggc ttc gta cag acg ctc gaa agc gtc ggc aaa ggc atc 1584 LysPhe Val Gly Phe Val Gln Thr Leu Glu Ser Val Gly Lys Gly Ile 515 520 525gat cag gcg caa agc agt ttt cag acg gca ttc aag caa ctt gcc gaa 1632 AspGln Ala Gln Ser Ser Phe Gln Thr Ala Phe Lys Gln Leu Ala Glu 530 535 540ggg cgc ggg aat ctg gtc gga cgc gcc gag aaa ctg cgt ctg ttg ggc 1680 GlyArg Gly Asn Leu Val Gly Arg Ala Glu Lys Leu Arg Leu Leu Gly 545 550 555560 gtg aag gca ggc aaa caa ctt caa cgg gat ttg gtc gag cgt tcc aat 1728Val Lys Ala Gly Lys Gln Leu Gln Arg Asp Leu Val Glu Arg Ser Asn 565 570575 gaa aca acg gcg ttg tcg gaa tct ttg gaa tac gcg gca gaa gat gaa 1776Glu Thr Thr Ala Leu Ser Glu Ser Leu Glu Tyr Ala Ala Glu Asp Glu 580 585590 gca gtc 1782 Ala Val 54 594 PRT Neisseria meningitidis 54 Met GluLeu Met Thr Val Leu Leu Pro Leu Ala Ala Leu Val Ser Gly 1 5 10 15 ValLeu Phe Thr Trp Leu Leu Met Lys Gly Arg Phe Gln Gly Glu Phe 20 25 30 AlaGly Leu Asn Ala His Leu Ala Glu Lys Ala Ala Arg Cys Asp Phe 35 40 45 ValGlu Gln Ala His Gly Lys Thr Val Ser Glu Leu Ala Val Leu Asp 50 55 60 GlyLys Tyr Arg His Leu Gln Asp Glu Asn Tyr Ala Leu Gly Asn Arg 65 70 75 80Phe Ser Ala Ala Glu Lys Gln Ile Ala His Leu Gln Glu Lys Glu Ala 85 90 95Glu Ser Ala Arg Leu Lys Gln Ser Tyr Ile Glu Leu Gln Glu Lys Ala 100 105110 Gln Gly Leu Ala Val Glu Asn Glu Arg Leu Ala Thr Gln Leu Gly Gln 115120 125 Glu Arg Lys Ala Phe Ala Asp Gln Tyr Ala Leu Glu Arg Gln Ile Arg130 135 140 Gln Arg Ile Glu Thr Asp Leu Glu Glu Ser Arg Gln Thr Val ArgAsp 145 150 155 160 Val Gln Asn Asp Leu Ser Asp Val Gly Asn Arg Phe AlaAla Ala Glu 165 170 175 Lys Gln Ile Ala His Leu Gln Glu Lys Glu Ala GluAla Glu Arg Leu 180 185 190 Arg Gln Ser His Thr Glu Leu Gln Glu Lys AlaGln Gly Leu Ala Val 195 200 205 Glu Asn Glu Arg Leu Ala Thr Gln Ile GluGln Glu Arg Leu Ala Ser 210 215 220 Glu Glu Lys Leu Ser Leu Leu Gly GluAla Arg Lys Ser Leu Ser Asp 225 230 235 240 Gln Phe Gln Asn Leu Ala AsnThr Ile Leu Glu Glu Lys Ser Arg Arg 245 250 255 Phe Thr Glu Gln Asn ArgGlu Gln Leu His Gln Val Leu Asn Pro Leu 260 265 270 Asn Glu Arg Ile HisGly Phe Gly Glu Leu Val Lys Gln Thr Tyr Asp 275 280 285 Lys Glu Ser ArgGlu Arg Leu Thr Leu Glu Asn Glu Leu Lys Arg Leu 290 295 300 Gln Gly LeuAsn Ala Gln Leu His Ser Glu Ala Lys Ala Leu Thr Asn 305 310 315 320 AlaLeu Thr Gly Thr Gln Asn Lys Val Gln Gly Asn Trp Gly Glu Met 325 330 335Ile Leu Glu Thr Val Leu Glu Asn Ser Gly Leu Gln Lys Gly Arg Glu 340 345350 Tyr Val Val Gln Ala Ala Ser Val Arg Lys Glu Glu Asp Gly Gly Thr 355360 365 Arg Arg Leu Gln Pro Asp Val Leu Val Asn Leu Pro Asp Asn Lys Gln370 375 380 Ile Val Ile Asp Ser Lys Val Ser Leu Thr Ala Tyr Val Arg TyrThr 385 390 395 400 Gln Ala Ala Asp Ala Asp Thr Ala Ala Arg Glu Leu AlaAla His Val 405 410 415 Ala Ser Ile Arg Ala His Met Lys Gly Leu Ser LeuLys Asp Tyr Thr 420 425 430 Asp Leu Glu Gly Val Asn Thr Leu Asp Phe ValPhe Met Phe Ile Pro 435 440 445 Val Glu Pro Ala Tyr Leu Leu Ala Leu GlnAsn Asp Ala Gly Leu Phe 450 455 460 Gln Glu Cys Phe Asp Lys Arg Ile MetLeu Val Gly Pro Ser Thr Leu 465 470 475 480 Leu Ala Thr Leu Arg Thr ValAla Asn Ile Trp Arg Asn Glu Gln Gln 485 490 495 Asn Gln Asn Ala Leu AlaIle Ala Asp Glu Gly Gly Lys Leu Tyr Asp 500 505 510 Lys Phe Val Gly PheVal Gln Thr Leu Glu Ser Val Gly Lys Gly Ile 515 520 525 Asp Gln Ala GlnSer Ser Phe Gln Thr Ala Phe Lys Gln Leu Ala Glu 530 535 540 Gly Arg GlyAsn Leu Val Gly Arg Ala Glu Lys Leu Arg Leu Leu Gly 545 550 555 560 ValLys Ala Gly Lys Gln Leu Gln Arg Asp Leu Val Glu Arg Ser Asn 565 570 575Glu Thr Thr Ala Leu Ser Glu Ser Leu Glu Tyr Ala Ala Glu Asp Glu 580 585590 Ala Val 55 819 DNA Neisseria meningitidis CDS (1)..(819) HemK 55 atgacg ttt gat aaa tgg ttg ggc ttg tca aaa ctg cct aaa aat gaa 48 Met ThrPhe Asp Lys Trp Leu Gly Leu Ser Lys Leu Pro Lys Asn Glu 1 5 10 15 gcaaga atg ctg ctg caa tat gtt tcg gaa tat acg cgc gtg cag ttg 96 Ala ArgMet Leu Leu Gln Tyr Val Ser Glu Tyr Thr Arg Val Gln Leu 20 25 30 ttg acgcgg ggc ggg gaa gaa atg ccg gac gaa gtc cga cag cgg gcg 144 Leu Thr ArgGly Gly Glu Glu Met Pro Asp Glu Val Arg Gln Arg Ala 35 40 45 gac agg ctggcg caa cgc cgt ctg aac ggc gag ccg gtt gcc tat att 192 Asp Arg Leu AlaGln Arg Arg Leu Asn Gly Glu Pro Val Ala Tyr Ile 50 55 60 tta ggt gcg cgcgaa ttt tat ggc aga cgc ttt aca gtc aac ccg agc 240 Leu Gly Ala Arg GluPhe Tyr Gly Arg Arg Phe Thr Val Asn Pro Ser 65 70 75 80 gtg ctg att ccgcgc ccc gaa acc gaa cat ttg gtc gaa gcc gta ttg 288 Val Leu Ile Pro ArgPro Glu Thr Glu His Leu Val Glu Ala Val Leu 85 90 95 gcg cgc ctg ccg gaaaac ggg cgc gtg tgg gat ttg ggg acg ggc agc 336 Ala Arg Leu Pro Glu AsnGly Arg Val Trp Asp Leu Gly Thr Gly Ser 100 105 110 gga gcg gtt gcc gtaacc gtc gcg ctc gaa cgc ccc gat gcg ttt gta 384 Gly Ala Val Ala Val ThrVal Ala Leu Glu Arg Pro Asp Ala Phe Val 115 120 125 cgc gca tcc gac atcagc ccg ccc gcc ctt gaa acg gcg cgg aaa aat 432 Arg Ala Ser Asp Ile SerPro Pro Ala Leu Glu Thr Ala Arg Lys Asn 130 135 140 gcg gca gat ttg ggcgcg cgg gtc gaa ttt gca tac ggt tcg tgg ttc 480 Ala Ala Asp Leu Gly AlaArg Val Glu Phe Ala Tyr Gly Ser Trp Phe 145 150 155 160 gac acc gat atgccg tct gaa ggg aaa tgg gac atc atc gtg tcc aac 528 Asp Thr Asp Met ProSer Glu Gly Lys Trp Asp Ile Ile Val Ser Asn 165 170 175 ccg ccc tat atcgaa aac ggc gat aaa cat ttg tcg caa ggc gat ttg 576 Pro Pro Tyr Ile GluAsn Gly Asp Lys His Leu Ser Gln Gly Asp Leu 180 185 190 cgg ttt gag ccgcaa atc gcg ctg acc gac ttt tca gac ggc cta agc 624 Arg Phe Glu Pro GlnIle Ala Leu Thr Asp Phe Ser Asp Gly Leu Ser 195 200 205 tgc atc cgc accttg gcg caa ggc gcg ccc gac cgt ttg gcg gag ggc 672 Cys Ile Arg Thr LeuAla Gln Gly Ala Pro Asp Arg Leu Ala Glu Gly 210 215 220 ggt ttt tta ttgctg gaa cac ggt ttc gat cag ggc gcg gcg gtg cgc 720 Gly Phe Leu Leu LeuGlu His Gly Phe Asp Gln Gly Ala Ala Val Arg 225 230 235 240 ggc gtg ttggcg gag aat ggt ttt tca gga gtg gaa acc ctg ccg gat 768 Gly Val Leu AlaGlu Asn Gly Phe Ser Gly Val Glu Thr Leu Pro Asp 245 250 255 ttg gcg ggtttg gac agg gtt acg ctg ggg aag tat atg aag cat ttg 816 Leu Ala Gly LeuAsp Arg Val Thr Leu Gly Lys Tyr Met Lys His Leu 260 265 270 aaa 819 Lys56 273 PRT Neisseria meningitidis 56 Met Thr Phe Asp Lys Trp Leu Gly LeuSer Lys Leu Pro Lys Asn Glu 1 5 10 15 Ala Arg Met Leu Leu Gln Tyr ValSer Glu Tyr Thr Arg Val Gln Leu 20 25 30 Leu Thr Arg Gly Gly Glu Glu MetPro Asp Glu Val Arg Gln Arg Ala 35 40 45 Asp Arg Leu Ala Gln Arg Arg LeuAsn Gly Glu Pro Val Ala Tyr Ile 50 55 60 Leu Gly Ala Arg Glu Phe Tyr GlyArg Arg Phe Thr Val Asn Pro Ser 65 70 75 80 Val Leu Ile Pro Arg Pro GluThr Glu His Leu Val Glu Ala Val Leu 85 90 95 Ala Arg Leu Pro Glu Asn GlyArg Val Trp Asp Leu Gly Thr Gly Ser 100 105 110 Gly Ala Val Ala Val ThrVal Ala Leu Glu Arg Pro Asp Ala Phe Val 115 120 125 Arg Ala Ser Asp IleSer Pro Pro Ala Leu Glu Thr Ala Arg Lys Asn 130 135 140 Ala Ala Asp LeuGly Ala Arg Val Glu Phe Ala Tyr Gly Ser Trp Phe 145 150 155 160 Asp ThrAsp Met Pro Ser Glu Gly Lys Trp Asp Ile Ile Val Ser Asn 165 170 175 ProPro Tyr Ile Glu Asn Gly Asp Lys His Leu Ser Gln Gly Asp Leu 180 185 190Arg Phe Glu Pro Gln Ile Ala Leu Thr Asp Phe Ser Asp Gly Leu Ser 195 200205 Cys Ile Arg Thr Leu Ala Gln Gly Ala Pro Asp Arg Leu Ala Glu Gly 210215 220 Gly Phe Leu Leu Leu Glu His Gly Phe Asp Gln Gly Ala Ala Val Arg225 230 235 240 Gly Val Leu Ala Glu Asn Gly Phe Ser Gly Val Glu Thr LeuPro Asp 245 250 255 Leu Ala Gly Leu Asp Arg Val Thr Leu Gly Lys Tyr MetLys His Leu 260 265 270 Lys 57 2148 DNA Neisseria meningitidis CDS(1)..(2148) NMB0287 57 atg ctc acc gat tta gaa aaa aac gcc atc cgc gaccat tac caa aac 48 Met Leu Thr Asp Leu Glu Lys Asn Ala Ile Arg Asp HisTyr Gln Asn 1 5 10 15 atc ggc aaa aac ctg ccc ggt ttc cgt ccg cgt gcttcg cag cgg gaa 96 Ile Gly Lys Asn Leu Pro Gly Phe Arg Pro Arg Ala SerGln Arg Glu 20 25 30 atg att gcg gcg gtt gcc aac gct ttt tcg cgg acg ttggcg cgc gaa 144 Met Ile Ala Ala Val Ala Asn Ala Phe Ser Arg Thr Leu AlaArg Glu 35 40 45 gaa ggc ggc gag ccg ccc aag cgc gaa ggc gag agc att gccgtg atc 192 Glu Gly Gly Glu Pro Pro Lys Arg Glu Gly Glu Ser Ile Ala ValIle 50 55 60 gaa ggg ccg acc ggc gtg ggc aaa tcg ttg gcc tac ctt ttg gccggc 240 Glu Gly Pro Thr Gly Val Gly Lys Ser Leu Ala Tyr Leu Leu Ala Gly65 70 75 80 ggc atc atg gcg caa aca cgc ggc aag cgg ctg att gtg agc agcgcg 288 Gly Ile Met Ala Gln Thr Arg Gly Lys Arg Leu Ile Val Ser Ser Ala85 90 95 acg gtt gcc ttg cag gag cag ttg gta gac cgc gac ctg ccg ttt ctg336 Thr Val Ala Leu Gln Glu Gln Leu Val Asp Arg Asp Leu Pro Phe Leu 100105 110 gtc gaa aaa agc ggt ttg gaa ctg acc ttc gca ctt gcc aaa ggg cgc384 Val Glu Lys Ser Gly Leu Glu Leu Thr Phe Ala Leu Ala Lys Gly Arg 115120 125 ggc cgc tat ctc tgc ccc tac aaa ctc tat cga ctg acg caa agc aat432 Gly Arg Tyr Leu Cys Pro Tyr Lys Leu Tyr Arg Leu Thr Gln Ser Asn 130135 140 gcc cag caa aac ctg ctc ggc ttt gaa gcc ccc gcc gtc ttg tgg gac480 Ala Gln Gln Asn Leu Leu Gly Phe Glu Ala Pro Ala Val Leu Trp Asp 145150 155 160 agc aaa ccc aag ccc gaa gaa ttg aag ctg ctg cgc gac atc gccgac 528 Ser Lys Pro Lys Pro Glu Glu Leu Lys Leu Leu Arg Asp Ile Ala Asp165 170 175 gaa ttt tcc gcc cga cgg ttc aac ggc gac cgc gac act tgg ccggaa 576 Glu Phe Ser Ala Arg Arg Phe Asn Gly Asp Arg Asp Thr Trp Pro Glu180 185 190 aaa atc gat gac gcg att tgg ctc aaa gtg acc aac gac cgc cacggc 624 Lys Ile Asp Asp Ala Ile Trp Leu Lys Val Thr Asn Asp Arg His Gly195 200 205 tgc ctg aaa acc gcc tgt ccc aac cgt ccg gaa tgt ccg ttt taccta 672 Cys Leu Lys Thr Ala Cys Pro Asn Arg Pro Glu Cys Pro Phe Tyr Leu210 215 220 gca cgc gat gtc ttg gaa acc gtc gat gtc gtc gtt gcc aac cacgat 720 Ala Arg Asp Val Leu Glu Thr Val Asp Val Val Val Ala Asn His Asp225 230 235 240 ctt ctg ctt gcc gac atc agt atg ggc ggc ggc gtg att ctgcct gcg 768 Leu Leu Leu Ala Asp Ile Ser Met Gly Gly Gly Val Ile Leu ProAla 245 250 255 ccc gaa aac agt ttc tat tgc atc gac gaa gcg cac cac ctgccc aaa 816 Pro Glu Asn Ser Phe Tyr Cys Ile Asp Glu Ala His His Leu ProLys 260 265 270 aaa gcc ctc agc cgt ttt gcc gcc gaa cat tca tgg aat attgcc gtt 864 Lys Ala Leu Ser Arg Phe Ala Ala Glu His Ser Trp Asn Ile AlaVal 275 280 285 tgg acg ctg gaa aaa ctg ccg cag ctg acc ggc aaa att gccgcg ctg 912 Trp Thr Leu Glu Lys Leu Pro Gln Leu Thr Gly Lys Ile Ala AlaLeu 290 295 300 acc gat aaa gcc gaa ctt gcc aac cta gcc gac gaa gcc gccgca tcc 960 Thr Asp Lys Ala Glu Leu Ala Asn Leu Ala Asp Glu Ala Ala AlaSer 305 310 315 320 ttg ctc gac agc ctg cat gaa tgg caa ttc cat ttg gcggaa gag ccg 1008 Leu Leu Asp Ser Leu His Glu Trp Gln Phe His Leu Ala GluGlu Pro 325 330 335 tct tta agt ctg ggg gtg tct gaa aac gac aga cga accaac agc gaa 1056 Ser Leu Ser Leu Gly Val Ser Glu Asn Asp Arg Arg Thr AsnSer Glu 340 345 350 ccg act tgg ctg tgg gaa gac ggc aaa atc ccc gaa ggcctc gaa acc 1104 Pro Thr Trp Leu Trp Glu Asp Gly Lys Ile Pro Glu Gly LeuGlu Thr 355 360 365 acc gtt tcc aat acg gcc att gct gcg cgc agc ctg ctcaaa cac gtt 1152 Thr Val Ser Asn Thr Ala Ile Ala Ala Arg Ser Leu Leu LysHis Val 370 375 380 atc ggg ctg aac gat gcg ctt tct gcc gca cgc cgc gaaaaa gaa cag 1200 Ile Gly Leu Asn Asp Ala Leu Ser Ala Ala Arg Arg Glu LysGlu Gln 385 390 395 400 gac ggc gcg ctc ctc gac cgc ctg acc agc gag ttcggt ctt ttt atc 1248 Asp Gly Ala Leu Leu Asp Arg Leu Thr Ser Glu Phe GlyLeu Phe Ile 405 410 415 gcc cgt atc gaa caa atc agc gcg gtt tgg gat ttgctc tcc act gtc 1296 Ala Arg Ile Glu Gln Ile Ser Ala Val Trp Asp Leu LeuSer Thr Val 420 425 430 ccc ctc gag ggt gaa gaa ccg ttg gcg aaa tgg ataacc cgc cgc gcc 1344 Pro Leu Glu Gly Glu Glu Pro Leu Ala Lys Trp Ile ThrArg Arg Ala 435 440 445 gac gac aaa aac gac tac att ttc aac gcc agc cccatc agc agc gca 1392 Asp Asp Lys Asn Asp Tyr Ile Phe Asn Ala Ser Pro IleSer Ser Ala 450 455 460 tcc cac ctt gcc aac agc ctg tgg cgg cgt gcg gcaggc gcg gta ttg 1440 Ser His Leu Ala Asn Ser Leu Trp Arg Arg Ala Ala GlyAla Val Leu 465 470 475 480 act tcc gcc acc ctg caa tcc ttg ggc aac ttcaac ctg atg ctg cgc 1488 Thr Ser Ala Thr Leu Gln Ser Leu Gly Asn Phe AsnLeu Met Leu Arg 485 490 495 caa acc ggg ctg caa tgg ctg ccc gaa acc accacc ctc gcc ctc aaa 1536 Gln Thr Gly Leu Gln Trp Leu Pro Glu Thr Thr ThrLeu Ala Leu Lys 500 505 510 agc ccc ttt gac ttt gaa aaa cag ggc gaa ctctac atc ccc ccc ata 1584 Ser Pro Phe Asp Phe Glu Lys Gln Gly Glu Leu TyrIle Pro Pro Ile 515 520 525 tac gcc agc ccc aaa gac ccc gaa gcc cac accgcc gcc gtc atc gaa 1632 Tyr Ala Ser Pro Lys Asp Pro Glu Ala His Thr AlaAla Val Ile Glu 530 535 540 tgg ctg ccc aag ctt att tcg ccc acc gaa gccatc ggc acg ctc gtc 1680 Trp Leu Pro Lys Leu Ile Ser Pro Thr Glu Ala IleGly Thr Leu Val 545 550 555 560 ttg ttt tcc tcg cgc aaa caa atg cag gatgtc gcc ctg cgc ctg ccc 1728 Leu Phe Ser Ser Arg Lys Gln Met Gln Asp ValAla Leu Arg Leu Pro 565 570 575 gga gac tac ctg ccg ctc ttg ctc gta caaggc gaa tta ccc aaa gcc 1776 Gly Asp Tyr Leu Pro Leu Leu Leu Val Gln GlyGlu Leu Pro Lys Ala 580 585 590 gtc ctc ctg caa aaa cac cac cgg gcc atagaa gaa ggc aaa gcc agc 1824 Val Leu Leu Gln Lys His His Arg Ala Ile GluGlu Gly Lys Ala Ser 595 600 605 atc atc ttc gga ctc gac agc ttt gcc gaagga ctc gac ctg ccc ggc 1872 Ile Ile Phe Gly Leu Asp Ser Phe Ala Glu GlyLeu Asp Leu Pro Gly 610 615 620 acc gcc tgc gtg caa gtc atc atc gcc aaactt ccc ttc gcc atg ccc 1920 Thr Ala Cys Val Gln Val Ile Ile Ala Lys LeuPro Phe Ala Met Pro 625 630 635 640 gac aac ccc atc gaa aaa acc caa aaccgc tgg ata gaa cag cgc ggc 1968 Asp Asn Pro Ile Glu Lys Thr Gln Asn ArgTrp Ile Glu Gln Arg Gly 645 650 655 ggc aac ccc ttc atc gaa atc acc gtcccc gaa gcc ggc atc aaa ctc 2016 Gly Asn Pro Phe Ile Glu Ile Thr Val ProGlu Ala Gly Ile Lys Leu 660 665 670 atc cag gcc gtc ggc cgc ctc atc cgcacc gaa caa gac tac ggc cgc 2064 Ile Gln Ala Val Gly Arg Leu Ile Arg ThrGlu Gln Asp Tyr Gly Arg 675 680 685 gta acc atc ctc gac aac cgc atc aaaaca cag cgg tac ggc caa caa 2112 Val Thr Ile Leu Asp Asn Arg Ile Lys ThrGln Arg Tyr Gly Gln Gln 690 695 700 tta ttg gcc ggc ctg ccg ccg ttt aaaagg ata ggg 2148 Leu Leu Ala Gly Leu Pro Pro Phe Lys Arg Ile Gly 705 710715 58 716 PRT Neisseria meningitidis 58 Met Leu Thr Asp Leu Glu Lys AsnAla Ile Arg Asp His Tyr Gln Asn 1 5 10 15 Ile Gly Lys Asn Leu Pro GlyPhe Arg Pro Arg Ala Ser Gln Arg Glu 20 25 30 Met Ile Ala Ala Val Ala AsnAla Phe Ser Arg Thr Leu Ala Arg Glu 35 40 45 Glu Gly Gly Glu Pro Pro LysArg Glu Gly Glu Ser Ile Ala Val Ile 50 55 60 Glu Gly Pro Thr Gly Val GlyLys Ser Leu Ala Tyr Leu Leu Ala Gly 65 70 75 80 Gly Ile Met Ala Gln ThrArg Gly Lys Arg Leu Ile Val Ser Ser Ala 85 90 95 Thr Val Ala Leu Gln GluGln Leu Val Asp Arg Asp Leu Pro Phe Leu 100 105 110 Val Glu Lys Ser GlyLeu Glu Leu Thr Phe Ala Leu Ala Lys Gly Arg 115 120 125 Gly Arg Tyr LeuCys Pro Tyr Lys Leu Tyr Arg Leu Thr Gln Ser Asn 130 135 140 Ala Gln GlnAsn Leu Leu Gly Phe Glu Ala Pro Ala Val Leu Trp Asp 145 150 155 160 SerLys Pro Lys Pro Glu Glu Leu Lys Leu Leu Arg Asp Ile Ala Asp 165 170 175Glu Phe Ser Ala Arg Arg Phe Asn Gly Asp Arg Asp Thr Trp Pro Glu 180 185190 Lys Ile Asp Asp Ala Ile Trp Leu Lys Val Thr Asn Asp Arg His Gly 195200 205 Cys Leu Lys Thr Ala Cys Pro Asn Arg Pro Glu Cys Pro Phe Tyr Leu210 215 220 Ala Arg Asp Val Leu Glu Thr Val Asp Val Val Val Ala Asn HisAsp 225 230 235 240 Leu Leu Leu Ala Asp Ile Ser Met Gly Gly Gly Val IleLeu Pro Ala 245 250 255 Pro Glu Asn Ser Phe Tyr Cys Ile Asp Glu Ala HisHis Leu Pro Lys 260 265 270 Lys Ala Leu Ser Arg Phe Ala Ala Glu His SerTrp Asn Ile Ala Val 275 280 285 Trp Thr Leu Glu Lys Leu Pro Gln Leu ThrGly Lys Ile Ala Ala Leu 290 295 300 Thr Asp Lys Ala Glu Leu Ala Asn LeuAla Asp Glu Ala Ala Ala Ser 305 310 315 320 Leu Leu Asp Ser Leu His GluTrp Gln Phe His Leu Ala Glu Glu Pro 325 330 335 Ser Leu Ser Leu Gly ValSer Glu Asn Asp Arg Arg Thr Asn Ser Glu 340 345 350 Pro Thr Trp Leu TrpGlu Asp Gly Lys Ile Pro Glu Gly Leu Glu Thr 355 360 365 Thr Val Ser AsnThr Ala Ile Ala Ala Arg Ser Leu Leu Lys His Val 370 375 380 Ile Gly LeuAsn Asp Ala Leu Ser Ala Ala Arg Arg Glu Lys Glu Gln 385 390 395 400 AspGly Ala Leu Leu Asp Arg Leu Thr Ser Glu Phe Gly Leu Phe Ile 405 410 415Ala Arg Ile Glu Gln Ile Ser Ala Val Trp Asp Leu Leu Ser Thr Val 420 425430 Pro Leu Glu Gly Glu Glu Pro Leu Ala Lys Trp Ile Thr Arg Arg Ala 435440 445 Asp Asp Lys Asn Asp Tyr Ile Phe Asn Ala Ser Pro Ile Ser Ser Ala450 455 460 Ser His Leu Ala Asn Ser Leu Trp Arg Arg Ala Ala Gly Ala ValLeu 465 470 475 480 Thr Ser Ala Thr Leu Gln Ser Leu Gly Asn Phe Asn LeuMet Leu Arg 485 490 495 Gln Thr Gly Leu Gln Trp Leu Pro Glu Thr Thr ThrLeu Ala Leu Lys 500 505 510 Ser Pro Phe Asp Phe Glu Lys Gln Gly Glu LeuTyr Ile Pro Pro Ile 515 520 525 Tyr Ala Ser Pro Lys Asp Pro Glu Ala HisThr Ala Ala Val Ile Glu 530 535 540 Trp Leu Pro Lys Leu Ile Ser Pro ThrGlu Ala Ile Gly Thr Leu Val 545 550 555 560 Leu Phe Ser Ser Arg Lys GlnMet Gln Asp Val Ala Leu Arg Leu Pro 565 570 575 Gly Asp Tyr Leu Pro LeuLeu Leu Val Gln Gly Glu Leu Pro Lys Ala 580 585 590 Val Leu Leu Gln LysHis His Arg Ala Ile Glu Glu Gly Lys Ala Ser 595 600 605 Ile Ile Phe GlyLeu Asp Ser Phe Ala Glu Gly Leu Asp Leu Pro Gly 610 615 620 Thr Ala CysVal Gln Val Ile Ile Ala Lys Leu Pro Phe Ala Met Pro 625 630 635 640 AspAsn Pro Ile Glu Lys Thr Gln Asn Arg Trp Ile Glu Gln Arg Gly 645 650 655Gly Asn Pro Phe Ile Glu Ile Thr Val Pro Glu Ala Gly Ile Lys Leu 660 665670 Ile Gln Ala Val Gly Arg Leu Ile Arg Thr Glu Gln Asp Tyr Gly Arg 675680 685 Val Thr Ile Leu Asp Asn Arg Ile Lys Thr Gln Arg Tyr Gly Gln Gln690 695 700 Leu Leu Ala Gly Leu Pro Pro Phe Lys Arg Ile Gly 705 710 71559 558 DNA Neisseria meningitidis CDS (1)..(558) sodC CDS 59 atg aat atgaaa acc tta tta gca cta gcg gtt agt gca gta tgt tca 48 Met Asn Met LysThr Leu Leu Ala Leu Ala Val Ser Ala Val Cys Ser 1 5 10 15 gtt ggt gttgcg caa gca cac gag cat aat acg ata cct aaa ggt gct 96 Val Gly Val AlaGln Ala His Glu His Asn Thr Ile Pro Lys Gly Ala 20 25 30 tct att gaa gtgaaa gtg caa caa ctt gat cca gta aac ggt aac aaa 144 Ser Ile Glu Val LysVal Gln Gln Leu Asp Pro Val Asn Gly Asn Lys 35 40 45 gat gtg ggt aca gtgact att act gaa tct aac tat ggt ctt gtg ttt 192 Asp Val Gly Thr Val ThrIle Thr Glu Ser Asn Tyr Gly Leu Val Phe 50 55 60 acc cct gat tta caa ggatta agc gaa ggc tta cat ggt ttc cac atc 240 Thr Pro Asp Leu Gln Gly LeuSer Glu Gly Leu His Gly Phe His Ile 65 70 75 80 cat gaa aac cca agc tgtgag cca aaa gaa aaa gaa ggt aaa ttg aca 288 His Glu Asn Pro Ser Cys GluPro Lys Glu Lys Glu Gly Lys Leu Thr 85 90 95 gct ggt tta ggc gca ggc ggtcac tgg gat cct aaa ggt gca aaa caa 336 Ala Gly Leu Gly Ala Gly Gly HisTrp Asp Pro Lys Gly Ala Lys Gln 100 105 110 cat ggt tac cca tgg caa gatgat gca cac tta ggt gat tta cct gca 384 His Gly Tyr Pro Trp Gln Asp AspAla His Leu Gly Asp Leu Pro Ala 115 120 125 tta act gta ttg cat gat ggcaca gca aca aat cct gtt tta gca cca 432 Leu Thr Val Leu His Asp Gly ThrAla Thr Asn Pro Val Leu Ala Pro 130 135 140 cgt ctt aaa cat tta gat gatgtt cgc ggt cac tct att atg atc cac 480 Arg Leu Lys His Leu Asp Asp ValArg Gly His Ser Ile Met Ile His 145 150 155 160 acg ggt ggt gat aat cactcc gat cat cca gct cca ctt ggc ggt ggc 528 Thr Gly Gly Asp Asn His SerAsp His Pro Ala Pro Leu Gly Gly Gly 165 170 175 ggc cca cgt atg gca tgtggc gtg att aaa 558 Gly Pro Arg Met Ala Cys Gly Val Ile Lys 180 185 60186 PRT Neisseria meningitidis 60 Met Asn Met Lys Thr Leu Leu Ala LeuAla Val Ser Ala Val Cys Ser 1 5 10 15 Val Gly Val Ala Gln Ala His GluHis Asn Thr Ile Pro Lys Gly Ala 20 25 30 Ser Ile Glu Val Lys Val Gln GlnLeu Asp Pro Val Asn Gly Asn Lys 35 40 45 Asp Val Gly Thr Val Thr Ile ThrGlu Ser Asn Tyr Gly Leu Val Phe 50 55 60 Thr Pro Asp Leu Gln Gly Leu SerGlu Gly Leu His Gly Phe His Ile 65 70 75 80 His Glu Asn Pro Ser Cys GluPro Lys Glu Lys Glu Gly Lys Leu Thr 85 90 95 Ala Gly Leu Gly Ala Gly GlyHis Trp Asp Pro Lys Gly Ala Lys Gln 100 105 110 His Gly Tyr Pro Trp GlnAsp Asp Ala His Leu Gly Asp Leu Pro Ala 115 120 125 Leu Thr Val Leu HisAsp Gly Thr Ala Thr Asn Pro Val Leu Ala Pro 130 135 140 Arg Leu Lys HisLeu Asp Asp Val Arg Gly His Ser Ile Met Ile His 145 150 155 160 Thr GlyGly Asp Asn His Ser Asp His Pro Ala Pro Leu Gly Gly Gly 165 170 175 GlyPro Arg Met Ala Cys Gly Val Ile Lys 180 185 61 1290 DNA Neisseriameningitidis CDS (1)..(1290) NMB0315 61 atg gct gtc ttc cca ctt tcg gcaaaa cat cgg aaa tac gcg ctg cgt 48 Met Ala Val Phe Pro Leu Ser Ala LysHis Arg Lys Tyr Ala Leu Arg 1 5 10 15 gcg ctt gcc gtt tcg att att ttggtg tcg gcg gca tac att gct tcg 96 Ala Leu Ala Val Ser Ile Ile Leu ValSer Ala Ala Tyr Ile Ala Ser 20 25 30 aca gag agg acg gag cgc gtc aga ccgcag cgc gtg gaa caa aat ctg 144 Thr Glu Arg Thr Glu Arg Val Arg Pro GlnArg Val Glu Gln Asn Leu 35 40 45 ccg ccg ctg tct tgg ggc ggc agc ggc gttcag acg gca tat tgg gtg 192 Pro Pro Leu Ser Trp Gly Gly Ser Gly Val GlnThr Ala Tyr Trp Val 50 55 60 cag gag gcg gtg cag ccg ggc gac tcg ctg gcggac gtg ctg gcg cgt 240 Gln Glu Ala Val Gln Pro Gly Asp Ser Leu Ala AspVal Leu Ala Arg 65 70 75 80 tcg ggt atg gcg cgg gac gag att gcc cga atcacg gaa aaa tat ggc 288 Ser Gly Met Ala Arg Asp Glu Ile Ala Arg Ile ThrGlu Lys Tyr Gly 85 90 95 ggc gaa gcc gat ttg cgg cat ttg cgt gcc gac cagtcg gtt cat gtt 336 Gly Glu Ala Asp Leu Arg His Leu Arg Ala Asp Gln SerVal His Val 100 105 110 ttg gtc ggc ggc gac ggc ggc gcg cgc gaa gtg cagttt ttt acc gac 384 Leu Val Gly Gly Asp Gly Gly Ala Arg Glu Val Gln PhePhe Thr Asp 115 120 125 gaa gac ggc gag cgc aat ctg gtc gct ttg gaa aagaaa ggc ggc ata 432 Glu Asp Gly Glu Arg Asn Leu Val Ala Leu Glu Lys LysGly Gly Ile 130 135 140 tgg cgg cgg tcg gct tct gag gcg gat atg aag gttttg ccg acg ctg 480 Trp Arg Arg Ser Ala Ser Glu Ala Asp Met Lys Val LeuPro Thr Leu 145 150 155 160 cgt tcg gtc gtg gtc aaa acg tcg gcg cgc ggttcg ctg gcg cgg gcg 528 Arg Ser Val Val Val Lys Thr Ser Ala Arg Gly SerLeu Ala Arg Ala 165 170 175 gaa gtg ccc gtc gaa atc cgc gaa tcc tta agcggg att ttc gcc ggc 576 Glu Val Pro Val Glu Ile Arg Glu Ser Leu Ser GlyIle Phe Ala Gly 180 185 190 cgc ttc agc ctt gac ggt ttg aag gaa ggc gatgcc gtg cgc ctg atg 624 Arg Phe Ser Leu Asp Gly Leu Lys Glu Gly Asp AlaVal Arg Leu Met 195 200 205 tac gac agc ctg tat ttc cac ggg cag cag gtggcg gcg ggc gat att 672 Tyr Asp Ser Leu Tyr Phe His Gly Gln Gln Val AlaAla Gly Asp Ile 210 215 220 ttg gcg gct gaa gtc gtt aag ggc ggc aca aggcat cag gcg ttc tat 720 Leu Ala Ala Glu Val Val Lys Gly Gly Thr Arg HisGln Ala Phe Tyr 225 230 235 240 tac cgt tcg gac aag gaa ggc gga ggg ggcggc aat tat tat gat gaa 768 Tyr Arg Ser Asp Lys Glu Gly Gly Gly Gly GlyAsn Tyr Tyr Asp Glu 245 250 255 gac ggc aag gtg ttg cag gaa aaa ggc ggcttc aac atc gag ccg ctg 816 Asp Gly Lys Val Leu Gln Glu Lys Gly Gly PheAsn Ile Glu Pro Leu 260 265 270 gtc tat acg cgc att tct tcg ccg ttc ggctac cgt atg cac ccc atc 864 Val Tyr Thr Arg Ile Ser Ser Pro Phe Gly TyrArg Met His Pro Ile 275 280 285 ctg cac aca tgg cgg ctg cac acg ggc atcgat tat gcc gca ccg cag 912 Leu His Thr Trp Arg Leu His Thr Gly Ile AspTyr Ala Ala Pro Gln 290 295 300 gga acg ccg gtc agg gct tcc gcc gac ggcgtg att acc ttt aaa ggc 960 Gly Thr Pro Val Arg Ala Ser Ala Asp Gly ValIle Thr Phe Lys Gly 305 310 315 320 cgg aag ggc gga tac ggc aac gcg gtgatg ata cgc cac gcc aac ggt 1008 Arg Lys Gly Gly Tyr Gly Asn Ala Val MetIle Arg His Ala Asn Gly 325 330 335 gtg gaa acg ctg tac gcg cac ttg agcgcg ttt tcg cag gcg gaa ggc 1056 Val Glu Thr Leu Tyr Ala His Leu Ser AlaPhe Ser Gln Ala Glu Gly 340 345 350 aat gtg cgc ggc ggc gag gtc atc ggtttt gtc ggt tcg acc ggg cgt 1104 Asn Val Arg Gly Gly Glu Val Ile Gly PheVal Gly Ser Thr Gly Arg 355 360 365 tcg acc ggg ccg cac ctg cat tac gaggcg cgc atc aac ggg cag ccc 1152 Ser Thr Gly Pro His Leu His Tyr Glu AlaArg Ile Asn Gly Gln Pro 370 375 380 gtc aat cct gtt tcg gtc gca ttg ccgaca ccg gaa ttg acg cag gcg 1200 Val Asn Pro Val Ser Val Ala Leu Pro ThrPro Glu Leu Thr Gln Ala 385 390 395 400 gac aag gcg gcg ttt gcc gcg cagaaa cag aag gcg gac gcg ctg ctt 1248 Asp Lys Ala Ala Phe Ala Ala Gln LysGln Lys Ala Asp Ala Leu Leu 405 410 415 gcg cgc ttg cgc ggc ata ccg gttacc gtg tcg caa tcg gat 1290 Ala Arg Leu Arg Gly Ile Pro Val Thr Val SerGln Ser Asp 420 425 430 62 430 PRT Neisseria meningitidis 62 Met Ala ValPhe Pro Leu Ser Ala Lys His Arg Lys Tyr Ala Leu Arg 1 5 10 15 Ala LeuAla Val Ser Ile Ile Leu Val Ser Ala Ala Tyr Ile Ala Ser 20 25 30 Thr GluArg Thr Glu Arg Val Arg Pro Gln Arg Val Glu Gln Asn Leu 35 40 45 Pro ProLeu Ser Trp Gly Gly Ser Gly Val Gln Thr Ala Tyr Trp Val 50 55 60 Gln GluAla Val Gln Pro Gly Asp Ser Leu Ala Asp Val Leu Ala Arg 65 70 75 80 SerGly Met Ala Arg Asp Glu Ile Ala Arg Ile Thr Glu Lys Tyr Gly 85 90 95 GlyGlu Ala Asp Leu Arg His Leu Arg Ala Asp Gln Ser Val His Val 100 105 110Leu Val Gly Gly Asp Gly Gly Ala Arg Glu Val Gln Phe Phe Thr Asp 115 120125 Glu Asp Gly Glu Arg Asn Leu Val Ala Leu Glu Lys Lys Gly Gly Ile 130135 140 Trp Arg Arg Ser Ala Ser Glu Ala Asp Met Lys Val Leu Pro Thr Leu145 150 155 160 Arg Ser Val Val Val Lys Thr Ser Ala Arg Gly Ser Leu AlaArg Ala 165 170 175 Glu Val Pro Val Glu Ile Arg Glu Ser Leu Ser Gly IlePhe Ala Gly 180 185 190 Arg Phe Ser Leu Asp Gly Leu Lys Glu Gly Asp AlaVal Arg Leu Met 195 200 205 Tyr Asp Ser Leu Tyr Phe His Gly Gln Gln ValAla Ala Gly Asp Ile 210 215 220 Leu Ala Ala Glu Val Val Lys Gly Gly ThrArg His Gln Ala Phe Tyr 225 230 235 240 Tyr Arg Ser Asp Lys Glu Gly GlyGly Gly Gly Asn Tyr Tyr Asp Glu 245 250 255 Asp Gly Lys Val Leu Gln GluLys Gly Gly Phe Asn Ile Glu Pro Leu 260 265 270 Val Tyr Thr Arg Ile SerSer Pro Phe Gly Tyr Arg Met His Pro Ile 275 280 285 Leu His Thr Trp ArgLeu His Thr Gly Ile Asp Tyr Ala Ala Pro Gln 290 295 300 Gly Thr Pro ValArg Ala Ser Ala Asp Gly Val Ile Thr Phe Lys Gly 305 310 315 320 Arg LysGly Gly Tyr Gly Asn Ala Val Met Ile Arg His Ala Asn Gly 325 330 335 ValGlu Thr Leu Tyr Ala His Leu Ser Ala Phe Ser Gln Ala Glu Gly 340 345 350Asn Val Arg Gly Gly Glu Val Ile Gly Phe Val Gly Ser Thr Gly Arg 355 360365 Ser Thr Gly Pro His Leu His Tyr Glu Ala Arg Ile Asn Gly Gln Pro 370375 380 Val Asn Pro Val Ser Val Ala Leu Pro Thr Pro Glu Leu Thr Gln Ala385 390 395 400 Asp Lys Ala Ala Phe Ala Ala Gln Lys Gln Lys Ala Asp AlaLeu Leu 405 410 415 Ala Arg Leu Arg Gly Ile Pro Val Thr Val Ser Gln SerAsp 420 425 430 63 603 DNA Neisseria meningitidis CDS (1)..(603) NMB111963 atg acc atc tat ttc aaa aac ggc ttt tac gac gac aca ttg ggc ggc 48Met Thr Ile Tyr Phe Lys Asn Gly Phe Tyr Asp Asp Thr Leu Gly Gly 1 5 1015 atc ccc gaa ggc gcg gtt gcc gtc cgc gcc gaa gaa tac gcc gcc ctt 96Ile Pro Glu Gly Ala Val Ala Val Arg Ala Glu Glu Tyr Ala Ala Leu 20 25 30ttg gca gga cag gcg cag ggc ggg cag att gcc gca gat tcc gac ggc 144 LeuAla Gly Gln Ala Gln Gly Gly Gln Ile Ala Ala Asp Ser Asp Gly 35 40 45 cgcccc gtt tta acc ccg ccg cgc ccg tcc gat tac cac gaa tgg gac 192 Arg ProVal Leu Thr Pro Pro Arg Pro Ser Asp Tyr His Glu Trp Asp 50 55 60 ggc aaaaaa tgg aaa atc agc aaa gcc gcc gcc gcc gcc cgt ttc gcc 240 Gly Lys LysTrp Lys Ile Ser Lys Ala Ala Ala Ala Ala Arg Phe Ala 65 70 75 80 aaa caaaaa acc gcc ttg gca ttc cgc ctc gcg gaa aag gcg gac gaa 288 Lys Gln LysThr Ala Leu Ala Phe Arg Leu Ala Glu Lys Ala Asp Glu 85 90 95 ctc aaa aacagc ctc ttg gcg ggc tat ccc caa gtg gaa atc gac agc 336 Leu Lys Asn SerLeu Leu Ala Gly Tyr Pro Gln Val Glu Ile Asp Ser 100 105 110 ttt tac aggcag gaa aaa gaa gcc ctc gcg cgg cag gcg gac aac aac 384 Phe Tyr Arg GlnGlu Lys Glu Ala Leu Ala Arg Gln Ala Asp Asn Asn 115 120 125 gcc ccg accccg atg ctg gcg caa atc gcc gcc gca agg ggc gtg gaa 432 Ala Pro Thr ProMet Leu Ala Gln Ile Ala Ala Ala Arg Gly Val Glu 130 135 140 ttg gac gttttg att gaa aaa gtt atc gaa aaa tcc gcc cgc ctg gct 480 Leu Asp Val LeuIle Glu Lys Val Ile Glu Lys Ser Ala Arg Leu Ala 145 150 155 160 gtt gccgcc ggc gcg att atc gga aag cgt cag cag ctc gaa gac aaa 528 Val Ala AlaGly Ala Ile Ile Gly Lys Arg Gln Gln Leu Glu Asp Lys 165 170 175 ttg aacacc atc gaa acc gcg ccc gga ttg gac gcg ctg gaa aag gaa 576 Leu Asn ThrIle Glu Thr Ala Pro Gly Leu Asp Ala Leu Glu Lys Glu 180 185 190 atc gaagaa tgg acg cta aac atc ggc 603 Ile Glu Glu Trp Thr Leu Asn Ile Gly 195200 64 201 PRT Neisseria meningitidis 64 Met Thr Ile Tyr Phe Lys Asn GlyPhe Tyr Asp Asp Thr Leu Gly Gly 1 5 10 15 Ile Pro Glu Gly Ala Val AlaVal Arg Ala Glu Glu Tyr Ala Ala Leu 20 25 30 Leu Ala Gly Gln Ala Gln GlyGly Gln Ile Ala Ala Asp Ser Asp Gly 35 40 45 Arg Pro Val Leu Thr Pro ProArg Pro Ser Asp Tyr His Glu Trp Asp 50 55 60 Gly Lys Lys Trp Lys Ile SerLys Ala Ala Ala Ala Ala Arg Phe Ala 65 70 75 80 Lys Gln Lys Thr Ala LeuAla Phe Arg Leu Ala Glu Lys Ala Asp Glu 85 90 95 Leu Lys Asn Ser Leu LeuAla Gly Tyr Pro Gln Val Glu Ile Asp Ser 100 105 110 Phe Tyr Arg Gln GluLys Glu Ala Leu Ala Arg Gln Ala Asp Asn Asn 115 120 125 Ala Pro Thr ProMet Leu Ala Gln Ile Ala Ala Ala Arg Gly Val Glu 130 135 140 Leu Asp ValLeu Ile Glu Lys Val Ile Glu Lys Ser Ala Arg Leu Ala 145 150 155 160 ValAla Ala Gly Ala Ile Ile Gly Lys Arg Gln Gln Leu Glu Asp Lys 165 170 175Leu Asn Thr Ile Glu Thr Ala Pro Gly Leu Asp Ala Leu Glu Lys Glu 180 185190 Ile Glu Glu Trp Thr Leu Asn Ile Gly 195 200 65 681 DNA Neisseriameningitidis CDS (1)..(681) NMB1119 65 atg ccg tct gaa aaa tgt tca gacggc att tct tat aac ctt ttg ttt 48 Met Pro Ser Glu Lys Cys Ser Asp GlyIle Ser Tyr Asn Leu Leu Phe 1 5 10 15 gca gca cat aac caa tca gtc tttcct att ccg ttc cga aat gca tac 96 Ala Ala His Asn Gln Ser Val Phe ProIle Pro Phe Arg Asn Ala Tyr 20 25 30 cat gcg ccc gta ccc aat att tgc gaacaa gga aag aaa atg gca cgt 144 His Ala Pro Val Pro Asn Ile Cys Glu GlnGly Lys Lys Met Ala Arg 35 40 45 tta acc gta cac acc ctc gaa acc gcc cccgaa gcc gcc aaa gcg cgc 192 Leu Thr Val His Thr Leu Glu Thr Ala Pro GluAla Ala Lys Ala Arg 50 55 60 gtc gag gcg gta ctt caa aac aac ggc ttt atcccc aac ctt atc ggc 240 Val Glu Ala Val Leu Gln Asn Asn Gly Phe Ile ProAsn Leu Ile Gly 65 70 75 80 gta tta tca aac gcc ccc gaa gcc ttg gcg ttttac caa gaa gtc ggc 288 Val Leu Ser Asn Ala Pro Glu Ala Leu Ala Phe TyrGln Glu Val Gly 85 90 95 aag ctc aac gcc gcc aac agc ctg acc gcc ggc gaagtc gaa gtc atc 336 Lys Leu Asn Ala Ala Asn Ser Leu Thr Ala Gly Glu ValGlu Val Ile 100 105 110 cag atc atc gcc gcc cgt acc aac caa tgc ggt ttttgc gtg gca ggg 384 Gln Ile Ile Ala Ala Arg Thr Asn Gln Cys Gly Phe CysVal Ala Gly 115 120 125 cac acc aaa ctc gca acc ctg aaa aaa ctc ctg tccgaa caa tcc gtc 432 His Thr Lys Leu Ala Thr Leu Lys Lys Leu Leu Ser GluGln Ser Val 130 135 140 aaa gcc gcg cgc gct ttg gca gca ggc gaa ttt gacgat gcc aaa ctc 480 Lys Ala Ala Arg Ala Leu Ala Ala Gly Glu Phe Asp AspAla Lys Leu 145 150 155 160 ggc gcg ctc gcc gcc ttc acc caa gcc gta atggcg aaa aaa ggc gcg 528 Gly Ala Leu Ala Ala Phe Thr Gln Ala Val Met AlaLys Lys Gly Ala 165 170 175 gta tcc gac gag gaa ctc aaa gca ttt ttc gatgcg ggc tac aac cag 576 Val Ser Asp Glu Glu Leu Lys Ala Phe Phe Asp AlaGly Tyr Asn Gln 180 185 190 cag cag gca gtc gaa gtc gtg atg ggc gta gccttg gca acc ctg tgc 624 Gln Gln Ala Val Glu Val Val Met Gly Val Ala LeuAla Thr Leu Cys 195 200 205 aac tac gtc aac aac ctc gga caa acc gaa atcaac ccc gaa ttg cag 672 Asn Tyr Val Asn Asn Leu Gly Gln Thr Glu Ile AsnPro Glu Leu Gln 210 215 220 gct tac gcc 681 Ala Tyr Ala 225 66 227 PRTNeisseria meningitidis 66 Met Pro Ser Glu Lys Cys Ser Asp Gly Ile SerTyr Asn Leu Leu Phe 1 5 10 15 Ala Ala His Asn Gln Ser Val Phe Pro IlePro Phe Arg Asn Ala Tyr 20 25 30 His Ala Pro Val Pro Asn Ile Cys Glu GlnGly Lys Lys Met Ala Arg 35 40 45 Leu Thr Val His Thr Leu Glu Thr Ala ProGlu Ala Ala Lys Ala Arg 50 55 60 Val Glu Ala Val Leu Gln Asn Asn Gly PheIle Pro Asn Leu Ile Gly 65 70 75 80 Val Leu Ser Asn Ala Pro Glu Ala LeuAla Phe Tyr Gln Glu Val Gly 85 90 95 Lys Leu Asn Ala Ala Asn Ser Leu ThrAla Gly Glu Val Glu Val Ile 100 105 110 Gln Ile Ile Ala Ala Arg Thr AsnGln Cys Gly Phe Cys Val Ala Gly 115 120 125 His Thr Lys Leu Ala Thr LeuLys Lys Leu Leu Ser Glu Gln Ser Val 130 135 140 Lys Ala Ala Arg Ala LeuAla Ala Gly Glu Phe Asp Asp Ala Lys Leu 145 150 155 160 Gly Ala Leu AlaAla Phe Thr Gln Ala Val Met Ala Lys Lys Gly Ala 165 170 175 Val Ser AspGlu Glu Leu Lys Ala Phe Phe Asp Ala Gly Tyr Asn Gln 180 185 190 Gln GlnAla Val Glu Val Val Met Gly Val Ala Leu Ala Thr Leu Cys 195 200 205 AsnTyr Val Asn Asn Leu Gly Gln Thr Glu Ile Asn Pro Glu Leu Gln 210 215 220Ala Tyr Ala 225 67 681 DNA Neisseria meningitidis CDS (1)..(681) NMB0995macrophage infectivity potentiator-related protein 67 atg ccg tct gaaaaa tgt tca gac ggc att tct tat aac ctt ttg ttt 48 Met Pro Ser Glu LysCys Ser Asp Gly Ile Ser Tyr Asn Leu Leu Phe 1 5 10 15 gca gca cat aaccaa tca gtc ttt cct att ccg ttc cga aat gca tac 96 Ala Ala His Asn GlnSer Val Phe Pro Ile Pro Phe Arg Asn Ala Tyr 20 25 30 cat gcg ccc gta cccaat att tgc gaa caa gga aag aaa atg gca cgt 144 His Ala Pro Val Pro AsnIle Cys Glu Gln Gly Lys Lys Met Ala Arg 35 40 45 tta acc gta cac acc ctcgaa acc gcc ccc gaa gcc gcc aaa gcg cgc 192 Leu Thr Val His Thr Leu GluThr Ala Pro Glu Ala Ala Lys Ala Arg 50 55 60 gtc gag gcg gta ctt caa aacaac ggc ttt atc ccc aac ctt atc ggc 240 Val Glu Ala Val Leu Gln Asn AsnGly Phe Ile Pro Asn Leu Ile Gly 65 70 75 80 gta tta tca aac gcc ccc gaagcc ttg gcg ttt tac caa gaa gtc ggc 288 Val Leu Ser Asn Ala Pro Glu AlaLeu Ala Phe Tyr Gln Glu Val Gly 85 90 95 aag ctc aac gcc gcc aac agc ctgacc gcc ggc gaa gtc gaa gtc atc 336 Lys Leu Asn Ala Ala Asn Ser Leu ThrAla Gly Glu Val Glu Val Ile 100 105 110 cag atc atc gcc gcc cgt acc aaccaa tgc ggt ttt tgc gtg gca ggg 384 Gln Ile Ile Ala Ala Arg Thr Asn GlnCys Gly Phe Cys Val Ala Gly 115 120 125 cac acc aaa ctc gca acc ctg aaaaaa ctc ctg tcc gaa caa tcc gtc 432 His Thr Lys Leu Ala Thr Leu Lys LysLeu Leu Ser Glu Gln Ser Val 130 135 140 aaa gcc gcg cgc gct ttg gca gcaggc gaa ttt gac gat gcc aaa ctc 480 Lys Ala Ala Arg Ala Leu Ala Ala GlyGlu Phe Asp Asp Ala Lys Leu 145 150 155 160 ggc gcg ctc gcc gcc ttc acccaa gcc gta atg gcg aaa aaa ggc gcg 528 Gly Ala Leu Ala Ala Phe Thr GlnAla Val Met Ala Lys Lys Gly Ala 165 170 175 gta tcc gac gag gaa ctc aaagca ttt ttc gat gcg ggc tac aac cag 576 Val Ser Asp Glu Glu Leu Lys AlaPhe Phe Asp Ala Gly Tyr Asn Gln 180 185 190 cag cag gca gtc gaa gtc gtgatg ggc gta gcc ttg gca acc ctg tgc 624 Gln Gln Ala Val Glu Val Val MetGly Val Ala Leu Ala Thr Leu Cys 195 200 205 aac tac gtc aac aac ctc ggacaa acc gaa atc aac ccc gaa ttg cag 672 Asn Tyr Val Asn Asn Leu Gly GlnThr Glu Ile Asn Pro Glu Leu Gln 210 215 220 gct tac gcc 681 Ala Tyr Ala225 68 227 PRT Neisseria meningitidis 68 Met Pro Ser Glu Lys Cys Ser AspGly Ile Ser Tyr Asn Leu Leu Phe 1 5 10 15 Ala Ala His Asn Gln Ser ValPhe Pro Ile Pro Phe Arg Asn Ala Tyr 20 25 30 His Ala Pro Val Pro Asn IleCys Glu Gln Gly Lys Lys Met Ala Arg 35 40 45 Leu Thr Val His Thr Leu GluThr Ala Pro Glu Ala Ala Lys Ala Arg 50 55 60 Val Glu Ala Val Leu Gln AsnAsn Gly Phe Ile Pro Asn Leu Ile Gly 65 70 75 80 Val Leu Ser Asn Ala ProGlu Ala Leu Ala Phe Tyr Gln Glu Val Gly 85 90 95 Lys Leu Asn Ala Ala AsnSer Leu Thr Ala Gly Glu Val Glu Val Ile 100 105 110 Gln Ile Ile Ala AlaArg Thr Asn Gln Cys Gly Phe Cys Val Ala Gly 115 120 125 His Thr Lys LeuAla Thr Leu Lys Lys Leu Leu Ser Glu Gln Ser Val 130 135 140 Lys Ala AlaArg Ala Leu Ala Ala Gly Glu Phe Asp Asp Ala Lys Leu 145 150 155 160 GlyAla Leu Ala Ala Phe Thr Gln Ala Val Met Ala Lys Lys Gly Ala 165 170 175Val Ser Asp Glu Glu Leu Lys Ala Phe Phe Asp Ala Gly Tyr Asn Gln 180 185190 Gln Gln Ala Val Glu Val Val Met Gly Val Ala Leu Ala Thr Leu Cys 195200 205 Asn Tyr Val Asn Asn Leu Gly Gln Thr Glu Ile Asn Pro Glu Leu Gln210 215 220 Ala Tyr Ala 225 69 939 DNA Neisseria meningitidis CDS(1)..(939) NMB0652 maf A 69 atg aaa acc ctg ctc ctc ctc atc ccc ctc gtcctc aca gcc tgc ggc 48 Met Lys Thr Leu Leu Leu Leu Ile Pro Leu Val LeuThr Ala Cys Gly 1 5 10 15 aca ctg acc ggc ata ccc gcc cac ggc ggc ggcaaa cgc ttt gcc gtc 96 Thr Leu Thr Gly Ile Pro Ala His Gly Gly Gly LysArg Phe Ala Val 20 25 30 gaa caa gaa ctc gtc gcc gca tcg tcc cgc gcc gccgtc aaa gaa atg 144 Glu Gln Glu Leu Val Ala Ala Ser Ser Arg Ala Ala ValLys Glu Met 35 40 45 gat ttg tcc gcc cta aaa gga cgc aaa gcc gcc ctt tacgtc tcc gtt 192 Asp Leu Ser Ala Leu Lys Gly Arg Lys Ala Ala Leu Tyr ValSer Val 50 55 60 atg ggc gac caa ggt tcg ggc aac ata agc ggc gga cgc tactct atc 240 Met Gly Asp Gln Gly Ser Gly Asn Ile Ser Gly Gly Arg Tyr SerIle 65 70 75 80 gac gca ctg ata cgc ggc ggc tac cac aac aac ccc gaa agtgcc acc 288 Asp Ala Leu Ile Arg Gly Gly Tyr His Asn Asn Pro Glu Ser AlaThr 85 90 95 caa tac agc tac ccc gcc tac gac act acc gcc acc acc aaa tccgac 336 Gln Tyr Ser Tyr Pro Ala Tyr Asp Thr Thr Ala Thr Thr Lys Ser Asp100 105 110 gcg ctc tcc agc gta acc act tcc aca tcg ctt ttg aac gcc cccgcc 384 Ala Leu Ser Ser Val Thr Thr Ser Thr Ser Leu Leu Asn Ala Pro Ala115 120 125 gcc gcc ctg acg aaa aac agc gga cgc aaa ggc gaa cgc tcc gccgga 432 Ala Ala Leu Thr Lys Asn Ser Gly Arg Lys Gly Glu Arg Ser Ala Gly130 135 140 ctg tcc gtc aac ggc acg ggc gac tac cgc aac gaa acc ctg ctcgcc 480 Leu Ser Val Asn Gly Thr Gly Asp Tyr Arg Asn Glu Thr Leu Leu Ala145 150 155 160 aac ccc cgc gac gtt tcc ttc ctg acc aac ctc atc caa accgtc ttc 528 Asn Pro Arg Asp Val Ser Phe Leu Thr Asn Leu Ile Gln Thr ValPhe 165 170 175 tac ctg cgc ggc atc gaa gtc gta ccg ccc gaa tac gcc gacacc gac 576 Tyr Leu Arg Gly Ile Glu Val Val Pro Pro Glu Tyr Ala Asp ThrAsp 180 185 190 gta ttc gta acc gtc gac gta ttc ggc acc gtc cgc agc cgtacc gaa 624 Val Phe Val Thr Val Asp Val Phe Gly Thr Val Arg Ser Arg ThrGlu 195 200 205 ctg cac ctc tac aac gcc gaa acc ctt aaa gcc caa acc aagctc gaa 672 Leu His Leu Tyr Asn Ala Glu Thr Leu Lys Ala Gln Thr Lys LeuGlu 210 215 220 tat ttc gcc gtt gac cgc gac agc cgg aaa ctg ctg att acccct aaa 720 Tyr Phe Ala Val Asp Arg Asp Ser Arg Lys Leu Leu Ile Thr ProLys 225 230 235 240 acc gcc gcc tac gaa tcc caa tac caa gaa caa tac gccctt tgg acc 768 Thr Ala Ala Tyr Glu Ser Gln Tyr Gln Glu Gln Tyr Ala LeuTrp Thr 245 250 255 ggc cct tac aaa gtc agc aaa acc gtc aaa gcc tca gaccgc ctg atg 816 Gly Pro Tyr Lys Val Ser Lys Thr Val Lys Ala Ser Asp ArgLeu Met 260 265 270 gtc gat ttc tcc gac att acc ccc tac ggc gac aca accgcc caa aac 864 Val Asp Phe Ser Asp Ile Thr Pro Tyr Gly Asp Thr Thr AlaGln Asn 275 280 285 cgt ccc gac ttc aaa caa aac aac ggt aaa aaa ccc gatgtc ggc aac 912 Arg Pro Asp Phe Lys Gln Asn Asn Gly Lys Lys Pro Asp ValGly Asn 290 295 300 gaa gtc atc cgc cgc cgc aaa gga gga 939 Glu Val IleArg Arg Arg Lys Gly Gly 305 310 70 313 PRT Neisseria meningitidis 70 MetLys Thr Leu Leu Leu Leu Ile Pro Leu Val Leu Thr Ala Cys Gly 1 5 10 15Thr Leu Thr Gly Ile Pro Ala His Gly Gly Gly Lys Arg Phe Ala Val 20 25 30Glu Gln Glu Leu Val Ala Ala Ser Ser Arg Ala Ala Val Lys Glu Met 35 40 45Asp Leu Ser Ala Leu Lys Gly Arg Lys Ala Ala Leu Tyr Val Ser Val 50 55 60Met Gly Asp Gln Gly Ser Gly Asn Ile Ser Gly Gly Arg Tyr Ser Ile 65 70 7580 Asp Ala Leu Ile Arg Gly Gly Tyr His Asn Asn Pro Glu Ser Ala Thr 85 9095 Gln Tyr Ser Tyr Pro Ala Tyr Asp Thr Thr Ala Thr Thr Lys Ser Asp 100105 110 Ala Leu Ser Ser Val Thr Thr Ser Thr Ser Leu Leu Asn Ala Pro Ala115 120 125 Ala Ala Leu Thr Lys Asn Ser Gly Arg Lys Gly Glu Arg Ser AlaGly 130 135 140 Leu Ser Val Asn Gly Thr Gly Asp Tyr Arg Asn Glu Thr LeuLeu Ala 145 150 155 160 Asn Pro Arg Asp Val Ser Phe Leu Thr Asn Leu IleGln Thr Val Phe 165 170 175 Tyr Leu Arg Gly Ile Glu Val Val Pro Pro GluTyr Ala Asp Thr Asp 180 185 190 Val Phe Val Thr Val Asp Val Phe Gly ThrVal Arg Ser Arg Thr Glu 195 200 205 Leu His Leu Tyr Asn Ala Glu Thr LeuLys Ala Gln Thr Lys Leu Glu 210 215 220 Tyr Phe Ala Val Asp Arg Asp SerArg Lys Leu Leu Ile Thr Pro Lys 225 230 235 240 Thr Ala Ala Tyr Glu SerGln Tyr Gln Glu Gln Tyr Ala Leu Trp Thr 245 250 255 Gly Pro Tyr Lys ValSer Lys Thr Val Lys Ala Ser Asp Arg Leu Met 260 265 270 Val Asp Phe SerAsp Ile Thr Pro Tyr Gly Asp Thr Thr Ala Gln Asn 275 280 285 Arg Pro AspPhe Lys Gln Asn Asn Gly Lys Lys Pro Asp Val Gly Asn 290 295 300 Glu ValIle Arg Arg Arg Lys Gly Gly 305 310 71 1308 DNA Neisseria meningitidisCDS (1)..(1308) NMB1876 N-acetyl glutamate synthetase 71 atg agc gcg cccgac ctc ttt gtc gcc cac ttc cgc gaa gcc gtc ccc 48 Met Ser Ala Pro AspLeu Phe Val Ala His Phe Arg Glu Ala Val Pro 1 5 10 15 tac atc cgc caaatg cgc ggc aaa acg ctg gtc gcc ggc ata gac gac 96 Tyr Ile Arg Gln MetArg Gly Lys Thr Leu Val Ala Gly Ile Asp Asp 20 25 30 cgc ctg ctc gaa ggtgat acc tta aac aag ctc gcc gcc gac atc ggg 144 Arg Leu Leu Glu Gly AspThr Leu Asn Lys Leu Ala Ala Asp Ile Gly 35 40 45 ctg ttg tcg caa ctg ggcatc agg ctc gtc ctc atc cac ggc gcg cgc 192 Leu Leu Ser Gln Leu Gly IleArg Leu Val Leu Ile His Gly Ala Arg 50 55 60 cac ttc ctc gac cgc cac gccgcc gct caa ggc cgc acg ccg cat tat 240 His Phe Leu Asp Arg His Ala AlaAla Gln Gly Arg Thr Pro His Tyr 65 70 75 80 tgc cgg ggc ttg cgc gtt accgac gaa acc tcg ctc gaa cag gcg cag 288 Cys Arg Gly Leu Arg Val Thr AspGlu Thr Ser Leu Glu Gln Ala Gln 85 90 95 cag ttt gcc ggc acc gtc cgc agccgt ttt gaa gcc gca ttg tgc ggc 336 Gln Phe Ala Gly Thr Val Arg Ser ArgPhe Glu Ala Ala Leu Cys Gly 100 105 110 agc gtt tcc ggg ttc gcg cgc gcgcct tcc gtc ccg ctc gta tcg ggc 384 Ser Val Ser Gly Phe Ala Arg Ala ProSer Val Pro Leu Val Ser Gly 115 120 125 aac ttc ctg acc gcc cgt ccg ataggt gtg att gac gga acc gat atg 432 Asn Phe Leu Thr Ala Arg Pro Ile GlyVal Ile Asp Gly Thr Asp Met 130 135 140 gaa tac gcg ggc gtt atc cgc aaaacc gac acc gcc gcc ctc cgt ttc 480 Glu Tyr Ala Gly Val Ile Arg Lys ThrAsp Thr Ala Ala Leu Arg Phe 145 150 155 160 caa ctc gac gcg ggc aat atcgtc tgg ctg ccg ccg ctc gga cat tcc 528 Gln Leu Asp Ala Gly Asn Ile ValTrp Leu Pro Pro Leu Gly His Ser 165 170 175 tac agc ggc aag acc ttc tatctc gat atg ctt caa acc gcc gcc tcc 576 Tyr Ser Gly Lys Thr Phe Tyr LeuAsp Met Leu Gln Thr Ala Ala Ser 180 185 190 gcc gcc gtc tcg ctt cag gccgaa aaa ctc gtt tac ctg acc ctt tca 624 Ala Ala Val Ser Leu Gln Ala GluLys Leu Val Tyr Leu Thr Leu Ser 195 200 205 gac ggc att tcc cgc ccc gacggc acg ctc gcc gaa acc ctc tcg gca 672 Asp Gly Ile Ser Arg Pro Asp GlyThr Leu Ala Glu Thr Leu Ser Ala 210 215 220 cag gaa gcg caa tcg ctg gcggaa cac gcc ggc ggc gaa acg cga cgg 720 Gln Glu Ala Gln Ser Leu Ala GluHis Ala Gly Gly Glu Thr Arg Arg 225 230 235 240 ctg att tcg tcc gcc gttgcc gcg ctc gaa ggc ggc gtg cat cgc gtc 768 Leu Ile Ser Ser Ala Val AlaAla Leu Glu Gly Gly Val His Arg Val 245 250 255 caa atc ctc aac gga gccgcc gac ggc agc ctg ctg caa gaa ctc ttc 816 Gln Ile Leu Asn Gly Ala AlaAsp Gly Ser Leu Leu Gln Glu Leu Phe 260 265 270 acc cgc aac ggc atc ggcacg tcc att gcc aaa gaa gcc ttc gtc tcc 864 Thr Arg Asn Gly Ile Gly ThrSer Ile Ala Lys Glu Ala Phe Val Ser 275 280 285 atc cgg cag gcg cac agcggc gac atc ccg cac atc gcc gcc ctc atc 912 Ile Arg Gln Ala His Ser GlyAsp Ile Pro His Ile Ala Ala Leu Ile 290 295 300 cgc ccg ctg gaa gaa cagggc atc ctg ctg cac cgc agc cgc gaa tac 960 Arg Pro Leu Glu Glu Gln GlyIle Leu Leu His Arg Ser Arg Glu Tyr 305 310 315 320 ctc gaa aac cac atttcc gaa ttt tcc atc ctc gaa cac gac ggc aac 1008 Leu Glu Asn His Ile SerGlu Phe Ser Ile Leu Glu His Asp Gly Asn 325 330 335 ctg tac ggt tgc gccgcc ctg aaa acc ttt gcc gaa gcc gat tgc ggc 1056 Leu Tyr Gly Cys Ala AlaLeu Lys Thr Phe Ala Glu Ala Asp Cys Gly 340 345 350 gaa atc gcc tgc cttgcc gtc tcg ccg cag gca cag gac ggc ggc tac 1104 Glu Ile Ala Cys Leu AlaVal Ser Pro Gln Ala Gln Asp Gly Gly Tyr 355 360 365 ggc gaa cgc ctg cttgcc cac att atc gat aag gcg cgc ggc ata ggc 1152 Gly Glu Arg Leu Leu AlaHis Ile Ile Asp Lys Ala Arg Gly Ile Gly 370 375 380 ata agc agg ctg ttcgca ctg tcc aca aat acc ggc gaa tgg ttt gcc 1200 Ile Ser Arg Leu Phe AlaLeu Ser Thr Asn Thr Gly Glu Trp Phe Ala 385 390 395 400 gaa cgc ggc tttcag acg gca tcg gaa gac gag ttg ccc gaa acg cgg 1248 Glu Arg Gly Phe GlnThr Ala Ser Glu Asp Glu Leu Pro Glu Thr Arg 405 410 415 cgc aaa gac taccgc agc aac gga cgg aac tcg cat att ctg gta cgt 1296 Arg Lys Asp Tyr ArgSer Asn Gly Arg Asn Ser His Ile Leu Val Arg 420 425 430 cgc ctg cac cgc1308 Arg Leu His Arg 435 72 436 PRT Neisseria meningitidis 72 Met SerAla Pro Asp Leu Phe Val Ala His Phe Arg Glu Ala Val Pro 1 5 10 15 TyrIle Arg Gln Met Arg Gly Lys Thr Leu Val Ala Gly Ile Asp Asp 20 25 30 ArgLeu Leu Glu Gly Asp Thr Leu Asn Lys Leu Ala Ala Asp Ile Gly 35 40 45 LeuLeu Ser Gln Leu Gly Ile Arg Leu Val Leu Ile His Gly Ala Arg 50 55 60 HisPhe Leu Asp Arg His Ala Ala Ala Gln Gly Arg Thr Pro His Tyr 65 70 75 80Cys Arg Gly Leu Arg Val Thr Asp Glu Thr Ser Leu Glu Gln Ala Gln 85 90 95Gln Phe Ala Gly Thr Val Arg Ser Arg Phe Glu Ala Ala Leu Cys Gly 100 105110 Ser Val Ser Gly Phe Ala Arg Ala Pro Ser Val Pro Leu Val Ser Gly 115120 125 Asn Phe Leu Thr Ala Arg Pro Ile Gly Val Ile Asp Gly Thr Asp Met130 135 140 Glu Tyr Ala Gly Val Ile Arg Lys Thr Asp Thr Ala Ala Leu ArgPhe 145 150 155 160 Gln Leu Asp Ala Gly Asn Ile Val Trp Leu Pro Pro LeuGly His Ser 165 170 175 Tyr Ser Gly Lys Thr Phe Tyr Leu Asp Met Leu GlnThr Ala Ala Ser 180 185 190 Ala Ala Val Ser Leu Gln Ala Glu Lys Leu ValTyr Leu Thr Leu Ser 195 200 205 Asp Gly Ile Ser Arg Pro Asp Gly Thr LeuAla Glu Thr Leu Ser Ala 210 215 220 Gln Glu Ala Gln Ser Leu Ala Glu HisAla Gly Gly Glu Thr Arg Arg 225 230 235 240 Leu Ile Ser Ser Ala Val AlaAla Leu Glu Gly Gly Val His Arg Val 245 250 255 Gln Ile Leu Asn Gly AlaAla Asp Gly Ser Leu Leu Gln Glu Leu Phe 260 265 270 Thr Arg Asn Gly IleGly Thr Ser Ile Ala Lys Glu Ala Phe Val Ser 275 280 285 Ile Arg Gln AlaHis Ser Gly Asp Ile Pro His Ile Ala Ala Leu Ile 290 295 300 Arg Pro LeuGlu Glu Gln Gly Ile Leu Leu His Arg Ser Arg Glu Tyr 305 310 315 320 LeuGlu Asn His Ile Ser Glu Phe Ser Ile Leu Glu His Asp Gly Asn 325 330 335Leu Tyr Gly Cys Ala Ala Leu Lys Thr Phe Ala Glu Ala Asp Cys Gly 340 345350 Glu Ile Ala Cys Leu Ala Val Ser Pro Gln Ala Gln Asp Gly Gly Tyr 355360 365 Gly Glu Arg Leu Leu Ala His Ile Ile Asp Lys Ala Arg Gly Ile Gly370 375 380 Ile Ser Arg Leu Phe Ala Leu Ser Thr Asn Thr Gly Glu Trp PheAla 385 390 395 400 Glu Arg Gly Phe Gln Thr Ala Ser Glu Asp Glu Leu ProGlu Thr Arg 405 410 415 Arg Lys Asp Tyr Arg Ser Asn Gly Arg Asn Ser HisIle Leu Val Arg 420 425 430 Arg Leu His Arg 435 73 712 PRT Neisseriameningitidis MISC_FEATURE TbpB 73 Met Asn Asn Pro Leu Val Asn Gln AlaAla Met Val Leu Pro Val Phe 1 5 10 15 Leu Leu Ser Ala Cys Leu Gly GlyGly Gly Ser Phe Asp Leu Asp Ser 20 25 30 Val Asp Thr Glu Ala Pro Arg ProAla Pro Lys Tyr Gln Asp Val Phe 35 40 45 Ser Glu Lys Pro Gln Ala Gln LysAsp Gln Gly Gly Tyr Gly Phe Ala 50 55 60 Met Arg Leu Lys Arg Arg Asn TrpTyr Pro Gln Ala Lys Glu Asp Glu 65 70 75 80 Val Lys Leu Asp Glu Ser AspTrp Glu Ala Thr Gly Leu Pro Asp Glu 85 90 95 Pro Lys Glu Leu Pro Lys ArgGln Lys Ser Val Ile Glu Lys Val Glu 100 105 110 Thr Asp Ser Asp Asn AsnIle Tyr Ser Ser Pro Tyr Leu Lys Pro Ser 115 120 125 Asn His Gln Asn GlyAsn Thr Gly Asn Gly Ile Asn Gln Pro Lys Asn 130 135 140 Gln Ala Lys AspTyr Glu Asn Phe Lys Tyr Val Tyr Ser Gly Trp Phe 145 150 155 160 Tyr LysHis Ala Lys Arg Glu Phe Asn Leu Lys Val Glu Pro Lys Ser 165 170 175 AlaLys Asn Gly Asp Asp Gly Tyr Ile Phe Tyr His Gly Lys Glu Pro 180 185 190Ser Arg Gln Leu Pro Ala Ser Gly Lys Ile Thr Tyr Lys Gly Val Trp 195 200205 His Phe Ala Thr Asp Thr Lys Lys Gly Gln Lys Phe Arg Glu Ile Ile 210215 220 Gln Pro Ser Lys Ser Gln Gly Asp Arg Tyr Ser Gly Phe Ser Gly Asp225 230 235 240 Asp Gly Glu Glu Tyr Ser Asn Lys Asn Lys Ser Thr Leu ThrAsp Gly 245 250 255 Gln Glu Gly Tyr Gly Phe Thr Ser Asn Leu Glu Val AspPhe His Asn 260 265 270 Lys Lys Leu Thr Gly Lys Leu Ile Arg Asn Asn AlaAsn Thr Asp Asn 275 280 285 Asn Gln Ala Thr Thr Thr Gln Tyr Tyr Ser LeuGlu Ala Gln Val Thr 290 295 300 Gly Asn Arg Phe Asn Gly Lys Ala Thr AlaThr Asp Lys Pro Gln Gln 305 310 315 320 Asn Ser Glu Thr Lys Glu His ProPhe Val Ser Asp Ser Ser Ser Leu 325 330 335 Ser Gly Gly Phe Phe Gly ProGln Gly Glu Glu Leu Gly Phe Arg Phe 340 345 350 Leu Ser Asp Asp Gln LysVal Ala Val Val Gly Ser Ala Lys Thr Lys 355 360 365 Asp Lys Pro Ala AsnGly Asn Thr Ala Ala Ala Ser Gly Gly Thr Asp 370 375 380 Ala Ala Ala SerAsn Gly Ala Ala Gly Thr Ser Ser Glu Asn Gly Lys 385 390 395 400 Leu ThrThr Val Leu Asp Ala Val Glu Leu Lys Leu Gly Asp Lys Glu 405 410 415 ValGln Lys Leu Asp Asn Phe Ser Asn Ala Ala Gln Leu Val Val Asp 420 425 430Gly Ile Met Ile Pro Leu Leu Pro Glu Ala Ser Glu Ser Gly Asn Asn 435 440445 Gln Ala Asn Gln Gly Thr Asn Gly Gly Thr Ala Phe Thr Arg Lys Phe 450455 460 Asp His Thr Pro Glu Ser Asp Lys Lys Asp Ala Gln Ala Gly Thr Gln465 470 475 480 Thr Asn Gly Ala Gln Thr Ala Ser Asn Thr Ala Gly Asp ThrAsn Gly 485 490 495 Lys Thr Lys Thr Tyr Glu Val Glu Val Cys Cys Ser AsnLeu Asn Tyr 500 505 510 Leu Lys Tyr Gly Met Leu Thr Arg Lys Asn Ser LysSer Ala Met Gln 515 520 525 Ala Gly Glu Ser Ser Ser Gln Ala Asp Ala LysThr Glu Gln Val Glu 530 535 540 Gln Ser Met Phe Leu Gln Gly Glu Arg ThrAsp Glu Lys Glu Ile Pro 545 550 555 560 Ser Glu Gln Asn Ile Val Tyr ArgGly Ser Trp Tyr Gly Tyr Ile Ala 565 570 575 Asn Asp Lys Ser Thr Ser TrpSer Gly Asn Ala Ser Asn Ala Thr Ser 580 585 590 Gly Asn Arg Ala Glu PheThr Val Asn Phe Ala Asp Lys Lys Ile Thr 595 600 605 Gly Thr Leu Thr AlaAsp Asn Arg Gln Glu Ala Thr Phe Thr Ile Asp 610 615 620 Gly Asn Ile LysAsp Asn Gly Phe Glu Gly Thr Ala Lys Thr Ala Glu 625 630 635 640 Ser GlyPhe Asp Leu Asp Gln Ser Asn Thr Thr Arg Thr Pro Lys Ala 645 650 655 TyrIle Thr Asp Ala Lys Val Gln Gly Gly Phe Tyr Gly Pro Lys Ala 660 665 670Glu Glu Leu Gly Gly Trp Phe Ala Tyr Pro Gly Asp Lys Gln Thr Lys 675 680685 Asn Ala Thr Asn Ala Ser Gly Asn Ser Ser Ala Thr Val Val Phe Gly 690695 700 Ala Lys Arg Gln Gln Pro Val Arg 705 710 74 67 DNA Neisseriameningitidis misc_feature porA promoter sequence 74 ttgaagacgtatcgggtgtt tgcccgatgt ttttaggttt ttatcaaatt tacaaaagga 60 agccgat 67 75253 PRT unidentified MISC_FEATURE kanamycin resistance 75 Met Asn GlyPro Ile Ile Met Thr Arg Glu Glu Arg Met Lys Ile Val 1 5 10 15 His GluIle Lys Glu Arg Ile Leu Asp Lys Tyr Gly Asp Asp Val Lys 20 25 30 Ala IleGly Val Tyr Gly Ser Leu Gly Arg Gln Thr Asp Gly Pro Tyr 35 40 45 Ser AspIle Glu Met Met Cys Val Met Ser Thr Glu Glu Ala Glu Phe 50 55 60 Ser HisGlu Trp Thr Thr Gly Glu Trp Lys Val Glu Val Asn Phe Asp 65 70 75 80 SerGlu Glu Ile Leu Leu Asp Tyr Ala Ser Gln Val Glu Ser Asp Trp 85 90 95 ProLeu Thr His Gly Gln Phe Phe Ser Ile Leu Pro Ile Tyr Asp Ser 100 105 110Gly Gly Tyr Leu Glu Lys Val Tyr Gln Thr Ala Lys Ser Val Glu Ala 115 120125 Gln Thr Phe His Asp Ala Ile Cys Ala Leu Ile Val Glu Glu Leu Phe 130135 140 Glu Tyr Ala Gly Lys Trp Arg Asn Ile Arg Val Gln Gly Pro Thr Thr145 150 155 160 Phe Leu Pro Ser Leu Thr Val Gln Val Ala Met Ala Gly AlaMet Leu 165 170 175 Ile Gly Leu His His Arg Ile Cys Tyr Thr Thr Ser AlaSer Val Leu 180 185 190 Thr Glu Ala Val Lys Gln Ser Asp Leu Pro Ser GlyTyr Asp His Leu 195 200 205 Cys Gln Phe Val Met Ser Gly Gln Leu Ser AspSer Glu Lys Leu Leu 210 215 220 Glu Ser Leu Glu Asn Phe Trp Asn Gly IleGln Glu Trp Thr Glu Arg 225 230 235 240 His Gly Tyr Ile Val Asp Val SerLys Arg Ile Pro Phe 245 250

What we claim is
 1. A composition comprising N. meningitidis outermembrane vesicles, wherein said outer membrane vesicles are enrichedwith at least one antigenic component.
 2. The composition of claim 1,wherein said antigenic component is an N. meningitidis antigenicprotein.
 3. The composition of claim 2, wherein said outer membranevesicles are from a first strain of N. meningitidis and said antigeniccomponent is from a second strain of N. meningitidis different from thefirst.
 4. The composition of claim 1, wherein said outer membranevesicles are enriched with a plurality of antigenic components fromdifferent strains of N. meningitidis.
 5. The composition of claim 1,wherein said outer membrane vesicles comprise a mixture of outermembrane vesicles from different strains of N. meningitidis.
 6. Thecomposition of claim 1, wherein said antigenic component is an N.meningitidis antigenic proteoglycan.
 7. The composition of claim 1,wherein said antigenic component is an N. meningitidis protein selectedfrom the group consisting of a surface antigen, a periplasmic protein, asuperoxide dismutase, and a glycoprotein.
 8. The composition of claim 1,wherein said antigenic component is selected from the group consistingof Cu,Zn-superoxide dismutase; neisserial surface protein A (NspA);porA; OMP85; FrpB; PilQ; Hsf; HemK; sodC; mafA; N-acetyl glutamatesynthetase; and macrophage infectivity potentiator-related protein. 9.The composition of claim 1, wherein said antigenic component is apeptide selected from the group consisting of SEQ ID NOS: 2; 4; 6; 8;10; 12; 14; 16; 18; 20; 22; 24; 26; 28; 30; 32; 34; 36; 38; 40; 42; 44;46; 48; 50; 52; 54; 56; 58; 60; 62; 64; 66; 68; 70; and
 72. 10. Thecomposition of claim 2, wherein said outer membrane vesicles are from afirst strain of N. meningitidis and said antigenic component is from asecond strain of N. meningitidis different from the first, furthercomprising a pharmaceutically acceptable carrier.
 11. A vaccinecomposition comprising outer membrane vesicles from a first strain of N.meningitidis, as well as an antigenic component from a second strain ofN. meningitidis different from the first, and a pharmaceuticallyacceptable carrier.
 12. The vaccine composition of claim 11, wherein theantigenic component is selected from the group consisting ofCu,Zn-superoxide dismutase; neisserial surface protein A (NspA); porA;OMP85; FrpB; PilQ; Hsf; HemK; sodC; mafA; N-acetyl glutamate synthetase;and macrophage infectivity potentiator-related protein.
 13. The vaccinecomposition of claim 11, wherein the antigenic component is a peptideselected from the group consisting of SEQ ID NOS: 2; 4; 6; 8; 10; 12;14; 16; 18; 20; 22; 24; 26; 28; 30; 32; 34; 36; 38; 40; 42; 44; 46; 48;50; 52; 54; 56; 58; 60; 62; 64; 66; 68; 70; and
 72. 14. A method ofmanufacture of a composition, comprising: (a) extracting an antigeniccomponent from an outer membrane of a bacteria, and preparing an aqueoussolution of said antigenic component; (b) extracting outer membranevesicles from a culture of N. meningitidis, and preparing an aqueoussolution of said outer membrane vesicles; (c) obtaining apharmaceutically acceptable carrier; and (d) admixing the solutionprepared in (a), the solution prepared in (b) and the carrier obtainedin (c).
 15. The method of claim 14, wherein said antigenic component isfrom a first strain of N. meningitidis, and said outer membrane vesiclesare from a second strain of N. meningitidis different from the first.16. The method of claim 15, wherein said strains of N. meningitidis areselected from the group consisting of K454; B16B6; L91 113; L91 705;L2412; 570059; L93 658; LAC 2043; H44/76; L91 543; L93 3215; A188/83;A321/83; L90-1252; L90-1493; LE-187; Y92-1009; Y92-1011; Y92-1012;Y92-1013; L93-1774; L93-1869; L932086; L93-2411; L93-2539; 310555;310626; L94/4931; F82/38; C-11(60E); ; N16; N91; N96; N97; MC58; MC58sod b-; MC58 sod c-; MC58 sod bc-; M97-251637; M97-251622; M97-251293;M97-251288; M97-251224; M97-251023; M97-250294; M97-250293; M97-250116;M96-255488; M96-255440; M96-254823; M96-253948; M96-253950; M96-255789;PhoP mutant; HG 09.02.76 Immunotype L3,7,9; ES 27.01.75 ImmunotypeL3,7,9; WY 23.01.65 Immunotype L3,7,9; MN 23.02.71; PKD 31.08.80; SG01.03.76; KH 21.05.67; JK 02.10.80; KN 01.06.77; SR 20.09.78; AG07.12.73 Immunotype L3,7,9; SSt 19.07.78; TM 16.12.79; SME 16.01.58; CG21.10.77 Immunotype L3,7,9; TB 12.03.74 Immunotype L3,7,9; FH 29.11.73Immunotype L3,7,9+L1,8,1; TF 17.04.76 Immunotype L3,7,9; CM 08.08.77Immunotype L3,7,9; KS 08.07.73 Immunotype L3,7,9; AS 06.11.77; LL03.01.75 Immunotype L3,7,9; SSk 29.07.76 Immunotype L3,7,9; GKY 24.05.66Immunotype L3,7,9; JHO 24.08.77 Immunotype L3,7,9; SGW 13.12.72Immunotype L3,7,9; AKS 28.09.75; MV 31.10.76; ID 15.02.74; SJ 28.06.83;JU 10.12.46; M99-240124; M99-240362; M99-240782; M99-241440; M99-241503;M99-241735; M99-242020; M99-242180; MC58 promo-; MC58 448.1 nov.; MC58432 nov; MC58 418 nov; MC58 401.2 nov; MC58424 nov; MC58 423.1 nov; MC58frp; MC58 abc; MC58 frp; MC58 comA; MC58 ner; MC58 hsp; M96 255789; M96255789; M96 255789; MC58; AR; LV; BM; JB; GN; SD (70942); G2379; L352;SH151; SH1789; SH1497; SH1602; SH 1717; SH148; L911134; SH155; SH161;J1755; SH4074; SH3424; J1455; SH1052; SH11114; M96 255789; M97-252455;M97-252535; M99-250591; M99-240706; M97-252005; Y92-1009; 9476; Z5005;Z6835; Z6244; Z3524; Z6466; Z8948; Z6904; Z4662; Z4673; Z7990; Z4683;Z4667; Z4707; Z6793; Z6784; Z7109; M97-251336; M97-252086; M97-252234;M97-252239; M97-252416; M97-252638; M98-251221; M98-251544; KG1O6;L91-543; and JNPHOPKO.
 17. The method of claim 14, wherein saidantigenic component is an N. meningitidis antigenic protein.
 18. Themethod of claim 14, wherein said antigenic component is an N.meningitidis antigenic proteoglycan.
 19. The method of claim 14, whereinsaid antigenic component is an N. meningitidis protein selected from thegroup consisting of a surface antigen, a periplasmic protein, asuperoxide dismutase, and a glycoprotein.
 20. The method of claim 14,wherein said antigenic component is selected from the group consistingof Cu,Zn-superoxide dismutase; neisserial surface protein A (NspA);porA; OMP85; FrpB; PilQ; Hsf; HemK; sodC; mafA; N-acetyl glutamatesynthetase; and macrophage infectivity potentiator-related protein. 21.The method of claim 14, wherein said antigenic component is a peptideselected from the group consisting of SEQ ID NOS: 2; 4; 6; 8; 10; 12;14; 16; 18; 20; 22; 24; 26; 28; 30; 32; 34; 36; 38; 40; 42; 44; 46; 48;50; 52; 54; 56; 58; 60; 62; 64; 66; 68; 70; and
 72. 22. A method ofmanufacture of a composition, comprising: (a) recombinantly expressing aDNA that encodes an antigenic component in a bacteria; (b) extractingsaid antigenic component from the outer membrane of said bacteria, andpreparing an aqueous solution of said antigenic component; (c)extracting outer membrane vesicles from a culture of N. meningitidis,and preparing an aqueous solution of said outer membrane vesicles; (d)obtaining a pharmaceutically acceptable carrier; and admixing thesolution prepared in (b), the solution prepared in (c) and the carrierobtained in (d).
 23. The method of claim 22, wherein said bacteria is astrain of N. meningitidis.
 24. A method of manufacture of a composition,comprising: (a) recombinantly expressing a DNA that encodes an antigeniccomponent in N. meningitidis; (b) extracting outer membrane vesiclesfrom said N. meningitidis, and preparing an aqueous solution of saidouter membrane vesicles, wherein said outer membrane vesicles comprisesaid antigenic component; (c) obtaining a pharmaceutically acceptablecarrier; and a. (d) admixing the solution prepared in (b), with thecarrier obtained in (c).
 25. The method of claim 24, wherein saidantigenic component is an N. meningitidis protein selected from thegroup consisting of a surface antigen, a periplasmic protein, asuperoxide dismutase, and a glycoprotein.
 26. The method of claim 24,wherein said antigenic component is an N. meningitidis protein selectedfrom the group consisting of Cu,Zn-superoxide dismutase; neisserialsurface protein A (NspA); porA; OMP85; FrpB; PilQ; Hsf; HemK; sodC;mafA; N-acetyl glutamate synthetase; and macrophage infectivitypotentiator-related protein.
 27. The method of claim 24, wherein saidantigenic component is an N. meningitidis protein selected from thegroup consisting of SEQ ID NOS: 2; 4; 6; 8; 10; 12; 14; 16; 18; 20; 22;24; 26; 28; 30; 32; 34; 36; 38; 40; 42; 44; 46; 48; 50; 52; 54; 56; 58;60; 62; 64; 66; 68; 70; and
 72. 28. A method of preventing N.meningitidis infection in an animal, comprising administering aneffective dose of a composition comprising an outer membrane vesicle andan antigenic component selected from the group consisting ofCu,Zn-superoxide dismutase; neisserial surface protein A (NspA); porA;OMP85; FrpB; PilQ; Hsf; HemK; sodC; mafA; N-acetyl glutamate synthetase;and macrophage infectivity potentiator-related protein, and apharmaceutically acceptable carrier.
 29. The method of claim 28, whereinwherein said antigenic component is from a first strain of N.meningitidis, and said outer membrane vesicles are from a second strainof N. meningitidis different from the first
 30. The method of claim 28,wherein said animal is a mammal.
 31. The method of claim 28, whereinsaid animal is a human.